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APDD_EMENI
ID   APDD_EMENI              Reviewed;         624 AA.
AC   Q5ATH0; C8VEB1;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=FAD-dependent monooxygenase apdD {ECO:0000303|PubMed:17369821};
DE            EC=1.-.-.- {ECO:0000305|PubMed:17369821};
DE   AltName: Full=Aspyridones biosynthesis protein D {ECO:0000303|PubMed:17369821};
GN   Name=apdD {ECO:0000303|PubMed:17369821}; ORFNames=AN8410;
OS   Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS   M139) (Aspergillus nidulans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=227321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=16372000; DOI=10.1038/nature04341;
RA   Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA   Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA   Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA   Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA   Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA   Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA   Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA   Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA   Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT   "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT   fumigatus and A. oryzae.";
RL   Nature 438:1105-1115(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA   Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA   Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA   Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA   Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA   Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA   Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA   Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA   van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA   Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA   Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA   Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA   Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA   Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA   van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA   Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA   Oliver S.G., Turner G.;
RT   "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT   effort.";
RL   Fungal Genet. Biol. 46:S2-13(2009).
RN   [3]
RP   FUNCTION, INDUCTION, AND PATHWAY.
RX   PubMed=17369821; DOI=10.1038/nchembio869;
RA   Bergmann S., Schuemann J., Scherlach K., Lange C., Brakhage A.A.,
RA   Hertweck C.;
RT   "Genomics-driven discovery of PKS-NRPS hybrid metabolites from Aspergillus
RT   nidulans.";
RL   Nat. Chem. Biol. 3:213-217(2007).
RN   [4]
RP   FUNCTION.
RX   PubMed=20828130; DOI=10.1021/ja107084d;
RA   Xu W., Cai X., Jung M.E., Tang Y.;
RT   "Analysis of intact and dissected fungal polyketide synthase-nonribosomal
RT   peptide synthetase in vitro and in Saccharomyces cerevisiae.";
RL   J. Am. Chem. Soc. 132:13604-13607(2010).
RN   [5]
RP   FUNCTION.
RX   DOI=10.1039/c3sc51785c;
RA   Wasil Z., Pahirulzaman K.A.K., Butts C., Simpson T.J., Lazarus C.M.,
RA   Cox R.J.;
RT   "One pathway, many compounds: Heterologous expression of a fungal
RT   biosynthetic pathway reveals its intrinsic potential for diversity.";
RL   Chem. Sci. 4:3845-3856(2013).
RN   [6]
RP   FUNCTION.
RX   PubMed=25494235; DOI=10.1021/ol503179v;
RA   Zou Y., Xu W., Tsunematsu Y., Tang M., Watanabe K., Tang Y.;
RT   "Methylation-dependent acyl transfer between polyketide synthase and
RT   nonribosomal peptide synthetase modules in fungal natural product
RT   biosynthesis.";
RL   Org. Lett. 16:6390-6393(2014).
CC   -!- FUNCTION: FAD-dependent monooxygenase; part of the gene cluster that
CC       mediates the biosynthesis of aspyridones (PubMed:17369821, Ref.5). The
CC       polyketide-amino acid backbone preaspyridone A is first assembled by
CC       the PKS-NRPS hybrid apdA (PubMed:17369821, PubMed:20828130). The
CC       assembly of preaspyridone A is initiated by loading of malonyl-CoA onto
CC       apdA, followed by decarboxylation to yield the acetyl starter unit
CC       (PubMed:20828130). The growing polyketide chain then elongates into a
CC       tetraketide (PubMed:20828130). The adpA PKS module catalyzes three
CC       Claisen condensations, as well as beta-keto processing and methylation
CC       (PubMed:17369821, PubMed:20828130). Alpha-methylation step during
CC       polyketide synthesis is a prerequisite and a key checkpoint for chain
CC       transfer between PKS and NRPS modules (PubMed:25494235). The downstream
CC       NRPS module contains the condensation (C), adenylation (A), and
CC       thiolation (T) domains and catalyzes the incorporation of tyrosine via
CC       the formation of the L-tyrosinyl-thioester and the amide linkage
CC       between L-tyrosinyl-thioester and the tetraketide (PubMed:20828130).
CC       The bimodular assembly line is terminated with a reductase (R) domain
CC       that facilitates formation and release of the tetramic acid product
CC       (PubMed:20828130). Because apdA lacks a designated enoylreductase (ER)
CC       domain, the required activity is provided the enoyl reductase apdC
CC       (PubMed:17369821, PubMed:20828130, Ref.5). ApdC appears to operate with
CC       different stereoselectivity in different PKS cycle (Ref.5). Combined
CC       with apdC, apdA is proposed to synthesize preaspyridone A via about 20
CC       enzymatic steps (PubMed:20828130). A number of oxidative steps
CC       performed successively by the cytochrome P450 monooxygenases apdE and
CC       apdB are required for the conversion of preaspyridone A to aspyridone A
CC       (PubMed:17369821). The cytochrome P450 monooxygenase apdE is
CC       responsible for the oxidative dephenylation of preaspyridone A (Ref.5).
CC       Finally, the predicted FAD-dependent monooxygenase apdD and the acyl-
CC       CoA dehydrogenase apdG may be involved in the transformation of
CC       aspyridone A into aspyridone B (PubMed:17369821) (Probable).
CC       {ECO:0000269|PubMed:17369821, ECO:0000269|PubMed:20828130,
CC       ECO:0000269|PubMed:25494235, ECO:0000269|Ref.5, ECO:0000305|Ref.5}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000305};
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:17369821}.
CC   -!- INDUCTION: Expression is positively regulated by the aspyridones
CC       cluster specific transcription regulator apdR (PubMed:17369821).
CC       {ECO:0000269|PubMed:17369821}.
CC   -!- SIMILARITY: Belongs to the paxM FAD-dependent monooxygenase family.
CC       {ECO:0000305}.
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DR   EMBL; BN001305; CBF80483.1; -; Genomic_DNA.
DR   EMBL; AACD01000153; EAA67032.1; -; Genomic_DNA.
DR   RefSeq; XP_681679.1; XM_676587.1.
DR   AlphaFoldDB; Q5ATH0; -.
DR   SMR; Q5ATH0; -.
DR   STRING; 162425.CADANIAP00002884; -.
DR   PRIDE; Q5ATH0; -.
DR   EnsemblFungi; CBF80483; CBF80483; ANIA_08410.
DR   EnsemblFungi; EAA67032; EAA67032; AN8410.2.
DR   GeneID; 2868841; -.
DR   KEGG; ani:AN8410.2; -.
DR   eggNOG; KOG3855; Eukaryota.
DR   HOGENOM; CLU_009665_14_2_1; -.
DR   InParanoid; Q5ATH0; -.
DR   OMA; SMPSEPW; -.
DR   OrthoDB; 366744at2759; -.
DR   Proteomes; UP000000560; Chromosome V.
DR   Proteomes; UP000005890; Unassembled WGS sequence.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0044550; P:secondary metabolite biosynthetic process; IEA:UniProt.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR002938; FAD-bd.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   Pfam; PF01494; FAD_binding_3; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   2: Evidence at transcript level;
KW   FAD; Flavoprotein; Monooxygenase; Oxidoreductase; Reference proteome.
FT   CHAIN           1..624
FT                   /note="FAD-dependent monooxygenase apdD"
FT                   /id="PRO_0000438454"
FT   BINDING         74..75
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:A6T923"
FT   BINDING         187
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:A6T923"
FT   BINDING         359
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:A6T923"
FT   BINDING         369..373
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:A6T923"
SQ   SEQUENCE   624 AA;  68876 MW;  03E338459AD28020 CRC64;
     MWSILSIKRS TSRMELMCLN AAECPINVFP EQQRNMPTES LNPGTVLIVG GGPVGLITAT
     TLAKYGVRSV ILERNLTTTK WPKMDLTNSR SMEIYQRLGI ADALRNVAVP SHYPFTCLFS
     SGLHADKAIT AWDLPSPDEY RRRIREQNDG SMPSEPWLRV SQEIFEAWLK ELGMENPLID
     FRAGWKVKGA RELDHGVEVE AIHSDTGEVW KVSADFVIGC DGAHSAIRKS LEIPLDGGPI
     HGYAVLVHFK SRDLSRIQKQ GQFWHLFFPN AASDGGSIKG AVIAQDEVDT WTVHRFMRPD
     VDHTQLSSEE IVYDLLGGMG GQPFPIRIDE VLVRSTWTPS VALARSYAGP KHRIFIAGDA
     CHQTVPTGGY GMNTGIADGY DIGWKLAAVI QGWAGPATLL SYEKERRPVG ELALQWSKVH
     MGNLMKMSAE LGLDAHMIEL NNETGAELRG AMHSYLQTHD GHNQSIGVEM GYRYVSNICV
     PGALDAELSP PEFHPRKYTP CTMPGYRAPH VYLTTGTPVS RLFGDGFTLV AFPEGEGLNA
     STEQLREAAR KKALPLEVVE LPGEMHAHEV WGASLVLVRP DGFVSWHGDS VRSQEEADRI
     IAQASGFDSE HLGNHVQAQE RSAL
 
 
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