IF2P_AERPE
ID IF2P_AERPE Reviewed; 617 AA.
AC Q9Y9B3;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Probable translation initiation factor IF-2;
GN Name=infB; OrderedLocusNames=APE_2374;
OS Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 /
OS K1).
OC Archaea; Crenarchaeota; Thermoprotei; Desulfurococcales;
OC Desulfurococcaceae; Aeropyrum.
OX NCBI_TaxID=272557;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1;
RX PubMed=10382966; DOI=10.1093/dnares/6.2.83;
RA Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K.,
RA Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A.,
RA Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S.,
RA Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A.,
RA Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H.;
RT "Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon,
RT Aeropyrum pernix K1.";
RL DNA Res. 6:83-101(1999).
CC -!- FUNCTION: Function in general translation initiation by promoting the
CC binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC along with eIF-2 (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000305}.
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DR EMBL; BA000002; BAA81387.1; -; Genomic_DNA.
DR PIR; C72466; C72466.
DR PDB; 5FG3; X-ray; 1.90 A; A=1-617.
DR PDB; 5YT0; X-ray; 1.89 A; A=1-358.
DR PDBsum; 5FG3; -.
DR PDBsum; 5YT0; -.
DR AlphaFoldDB; Q9Y9B3; -.
DR SMR; Q9Y9B3; -.
DR STRING; 272557.APE_2374; -.
DR PRIDE; Q9Y9B3; -.
DR EnsemblBacteria; BAA81387; BAA81387; APE_2374.
DR KEGG; ape:APE_2374; -.
DR PATRIC; fig|272557.25.peg.1584; -.
DR eggNOG; arCOG01560; Archaea.
DR OMA; FRQSKPA; -.
DR Proteomes; UP000002518; Chromosome.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.10050; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00100_A; IF_2_A; 1.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR029459; EFTU-type.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR004544; TF_aIF-2_arc.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR43381; PTHR43381; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF14578; GTP_EFTU_D4; 1.
DR Pfam; PF11987; IF-2; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF52156; SSF52156; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00491; aIF-2; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; GTP-binding; Initiation factor; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome.
FT CHAIN 1..617
FT /note="Probable translation initiation factor IF-2"
FT /id="PRO_0000137297"
FT DOMAIN 14..231
FT /note="tr-type G"
FT REGION 23..30
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 48..52
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 87..90
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 141..144
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 209..211
FT /note="G5"
FT /evidence="ECO:0000250"
FT BINDING 23..30
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 87..91
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 141..144
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT STRAND 18..23
FT /evidence="ECO:0007829|PDB:5YT0"
FT HELIX 29..42
FT /evidence="ECO:0007829|PDB:5YT0"
FT STRAND 50..52
FT /evidence="ECO:0007829|PDB:5YT0"
FT STRAND 55..59
FT /evidence="ECO:0007829|PDB:5YT0"
FT HELIX 60..66
FT /evidence="ECO:0007829|PDB:5YT0"
FT HELIX 68..70
FT /evidence="ECO:0007829|PDB:5YT0"
FT TURN 71..73
FT /evidence="ECO:0007829|PDB:5YT0"
FT STRAND 82..88
FT /evidence="ECO:0007829|PDB:5YT0"
FT HELIX 91..95
FT /evidence="ECO:0007829|PDB:5YT0"
FT STRAND 97..99
FT /evidence="ECO:0007829|PDB:5FG3"
FT STRAND 102..104
FT /evidence="ECO:0007829|PDB:5FG3"
FT STRAND 106..113
FT /evidence="ECO:0007829|PDB:5YT0"
FT TURN 114..116
FT /evidence="ECO:0007829|PDB:5YT0"
FT HELIX 120..132
FT /evidence="ECO:0007829|PDB:5YT0"
FT STRAND 136..141
FT /evidence="ECO:0007829|PDB:5YT0"
FT HELIX 143..145
FT /evidence="ECO:0007829|PDB:5YT0"
FT HELIX 157..162
FT /evidence="ECO:0007829|PDB:5YT0"
FT HELIX 166..185
FT /evidence="ECO:0007829|PDB:5YT0"
FT STRAND 190..192
FT /evidence="ECO:0007829|PDB:5YT0"
FT HELIX 193..195
FT /evidence="ECO:0007829|PDB:5YT0"
FT TURN 199..201
FT /evidence="ECO:0007829|PDB:5YT0"
FT STRAND 202..207
FT /evidence="ECO:0007829|PDB:5YT0"
FT TURN 210..212
FT /evidence="ECO:0007829|PDB:5YT0"
FT HELIX 216..230
FT /evidence="ECO:0007829|PDB:5YT0"
FT HELIX 232..235
FT /evidence="ECO:0007829|PDB:5YT0"
FT STRAND 243..252
FT /evidence="ECO:0007829|PDB:5YT0"
FT TURN 253..255
FT /evidence="ECO:0007829|PDB:5YT0"
FT STRAND 256..269
FT /evidence="ECO:0007829|PDB:5YT0"
FT STRAND 273..278
FT /evidence="ECO:0007829|PDB:5YT0"
FT STRAND 281..286
FT /evidence="ECO:0007829|PDB:5YT0"
FT STRAND 289..292
FT /evidence="ECO:0007829|PDB:5YT0"
FT HELIX 299..301
FT /evidence="ECO:0007829|PDB:5FG3"
FT STRAND 307..321
FT /evidence="ECO:0007829|PDB:5YT0"
FT STRAND 333..339
FT /evidence="ECO:0007829|PDB:5YT0"
FT HELIX 340..357
FT /evidence="ECO:0007829|PDB:5YT0"
FT STRAND 358..360
FT /evidence="ECO:0007829|PDB:5FG3"
FT STRAND 362..364
FT /evidence="ECO:0007829|PDB:5FG3"
FT STRAND 366..372
FT /evidence="ECO:0007829|PDB:5FG3"
FT HELIX 373..386
FT /evidence="ECO:0007829|PDB:5FG3"
FT STRAND 390..398
FT /evidence="ECO:0007829|PDB:5FG3"
FT HELIX 400..412
FT /evidence="ECO:0007829|PDB:5FG3"
FT HELIX 414..416
FT /evidence="ECO:0007829|PDB:5FG3"
FT STRAND 418..423
FT /evidence="ECO:0007829|PDB:5FG3"
FT HELIX 428..437
FT /evidence="ECO:0007829|PDB:5FG3"
FT STRAND 440..446
FT /evidence="ECO:0007829|PDB:5FG3"
FT HELIX 447..470
FT /evidence="ECO:0007829|PDB:5FG3"
FT STRAND 477..504
FT /evidence="ECO:0007829|PDB:5FG3"
FT STRAND 509..511
FT /evidence="ECO:0007829|PDB:5FG3"
FT STRAND 517..525
FT /evidence="ECO:0007829|PDB:5FG3"
FT STRAND 539..547
FT /evidence="ECO:0007829|PDB:5FG3"
FT TURN 550..552
FT /evidence="ECO:0007829|PDB:5FG3"
FT STRAND 559..562
FT /evidence="ECO:0007829|PDB:5FG3"
FT HELIX 566..575
FT /evidence="ECO:0007829|PDB:5FG3"
FT TURN 577..579
FT /evidence="ECO:0007829|PDB:5FG3"
FT HELIX 582..597
FT /evidence="ECO:0007829|PDB:5FG3"
FT HELIX 601..609
FT /evidence="ECO:0007829|PDB:5FG3"
SQ SEQUENCE 617 AA; 68521 MW; 493DC149C6028EB3 CRC64;
MAGDKGGGDG ERRLRQPIVV VLGHVDHGKT TLLDKIRRTA VAAKEAGGIT QHIGASIVPA
DVIEKIAEPL KKVIPVKLVI PGLLFIDTPG HELFSNLRRR GGSVADFAIL VVDIMEGFKP
QTYEALELLK ERRVPFLIAA NKIDRIPGWK PNPDAPFIET IRRQDPKVRE ILEQRVYEIV
GKMYEAGLPA ELFTRIKDFR RKIAIVPVSA RTGEGIPELL AVLAGLTQTY LKERLRYAEG
PAKGVVLEVK EMQGFGTVVD AVIYDGVLKK EDIIVVGGRE GPIVTRVRAL LMPAPLQDIR
SREARFVQVD RVYAAAGVRI AAPGLDDVIA GSPIYAAESE EEARKLMEAV QREIEELRFR
TENIGVVVKA DTLGTLEALV EALRRRGVPV RLADIGPVSR SDVLDAAVTR KIDPYLGVVL
AFNVKVLPEA EEEASRAGVK IFRESMIYKL IEDYEEWVKK EKEAERLKAL NSLIRPGKFR
ILPGYVFRRS DPAIVGVEVL GGVIRPGYPV MDSQGRELGR IMAIKDRDRS LEEARLGAAV
AVSIQGRILI GRHANEGDIL YTNVPAQHAY KILTEFKDLV SKDELDVLRE IAEIKRRAAD
HEYNKVLLRL KIKRVSQ
