ID   IF2P_METAR              Reviewed;         594 AA.
AC   Q0W8X2;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Probable translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN   Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=UNCMA_30180;
GN   ORFNames=LRC142;
OS   Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanocellales; Methanocellaceae; Methanocella.
OX   NCBI_TaxID=351160;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 22066 / NBRC 105507 / MRE50;
RX   PubMed=16857943; DOI=10.1126/science.1127062;
RA   Erkel C., Kube M., Reinhardt R., Liesack W.;
RT   "Genome of rice cluster I archaea -- the key methane producers in the rice
RT   rhizosphere.";
RL   Science 313:370-372(2006).
CC   -!- FUNCTION: Function in general translation initiation by promoting the
CC       binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC       along with eIF-2. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00100}.
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DR   EMBL; AM114193; CAJ35154.1; -; Genomic_DNA.
DR   RefSeq; WP_012037333.1; NC_009464.1.
DR   AlphaFoldDB; Q0W8X2; -.
DR   SMR; Q0W8X2; -.
DR   STRING; 351160.LRC142; -.
DR   PRIDE; Q0W8X2; -.
DR   EnsemblBacteria; CAJ35154; CAJ35154; LRC142.
DR   GeneID; 5143926; -.
DR   KEGG; rci:LRC142; -.
DR   PATRIC; fig|351160.9.peg.3107; -.
DR   eggNOG; arCOG01560; Archaea.
DR   OMA; FRQSKPA; -.
DR   OrthoDB; 17053at2157; -.
DR   Proteomes; UP000000663; Chromosome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10050; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00100_A; IF_2_A; 1.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR029459; EFTU-type.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR004544; TF_aIF-2_arc.
DR   InterPro; IPR015760; TIF_IF2.
DR   InterPro; IPR023115; TIF_IF2_dom3.
DR   InterPro; IPR036925; TIF_IF2_dom3_sf.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   PANTHER; PTHR43381; PTHR43381; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF14578; GTP_EFTU_D4; 1.
DR   Pfam; PF11987; IF-2; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF52156; SSF52156; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00491; aIF-2; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Initiation factor; Nucleotide-binding; Protein biosynthesis;
KW   Reference proteome.
FT   CHAIN           1..594
FT                   /note="Probable translation initiation factor IF-2"
FT                   /id="PRO_0000335537"
FT   DOMAIN          11..226
FT                   /note="tr-type G"
FT   REGION          20..27
FT                   /note="G1"
FT                   /evidence="ECO:0000250"
FT   REGION          45..49
FT                   /note="G2"
FT                   /evidence="ECO:0000250"
FT   REGION          81..84
FT                   /note="G3"
FT                   /evidence="ECO:0000250"
FT   REGION          135..138
FT                   /note="G4"
FT                   /evidence="ECO:0000250"
FT   REGION          203..205
FT                   /note="G5"
FT                   /evidence="ECO:0000250"
FT   BINDING         20..27
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         81..85
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         135..138
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ   SEQUENCE   594 AA;  65912 MW;  1A127E420DDC079C CRC64;
     MATATEIRKD LRTPIVCVMG HVDHGKTSLL DRIRGTAVVD KEAGAITQHI GATEVPLQTI
     QTLCKGMIGG NIVVPGLLFI DTPGHHAFTN LRSRGGALAD LAVLVVDINE GFQPQTVEAI
     KILKQFKTPF VIAANKIDRI HGWTAKNNSP FLQTFNSQPD HVKGIIETKT YELVGRMSDL
     GFSSDRYDRI RDFTRNIGII PISARTGEGI PDLLMILIGL AQRFLEESLK FQVTGPGVGT
     ILEVKEERGL GYTIDTIIYD GEIRVGDTIV IGGREKPYST KVRALLKPKP NREIRVEERF
     DRVNKVTAAS GVKILAPELE KAMAGSQVRV TKESNVEDII KEIEQEMEQA KIVTDEVGVM
     VKADTLGSLE AIVNELREAK IPIGRADVGD ISKRDIINAE TVNDPMYRVM LGFNVTILPD
     ANDYLQTTDI KIFNSDVIYH LIDDFRKWET EQRALAEKKK FAEIVRPGKV KYLPNCTFRQ
     SKPAVIGLQI MGGMLKPGVT LIKPDGSKIG VVRQIQERNE NISIATVGKE VAVSIDGPTA
     GRQINEGEIY FVDVPEGHSK VLEFQLKDTI KQDELETLME FLAIKRKDNP FWGR