ID   IF2P_METHJ              Reviewed;         604 AA.
AC   Q2FU48;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Probable translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN   Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=Mhun_2489;
OS   Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 /
OS   JF-1).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanomicrobiales; Methanospirillaceae; Methanospirillum.
OX   NCBI_TaxID=323259;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27890 / DSM 864 / NBRC 100397 / JF-1;
RX   PubMed=26744606; DOI=10.1186/s40793-015-0124-8;
RA   Gunsalus R.P., Cook L.E., Crable B., Rohlin L., McDonald E., Mouttaki H.,
RA   Sieber J.R., Poweleit N., Zhou H., Lapidus A.L., Daligault H.E., Land M.,
RA   Gilna P., Ivanova N., Kyrpides N., Culley D.E., McInerney M.J.;
RT   "Complete genome sequence of Methanospirillum hungatei type strain JF1.";
RL   Stand. Genomic Sci. 11:2-2(2016).
CC   -!- FUNCTION: Function in general translation initiation by promoting the
CC       binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC       along with eIF-2. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000254; ABD42189.1; -; Genomic_DNA.
DR   RefSeq; WP_011449447.1; NC_007796.1.
DR   AlphaFoldDB; Q2FU48; -.
DR   SMR; Q2FU48; -.
DR   STRING; 323259.Mhun_2489; -.
DR   EnsemblBacteria; ABD42189; ABD42189; Mhun_2489.
DR   GeneID; 3924794; -.
DR   KEGG; mhu:Mhun_2489; -.
DR   eggNOG; arCOG01560; Archaea.
DR   HOGENOM; CLU_002656_3_3_2; -.
DR   OMA; FRQSKPA; -.
DR   OrthoDB; 17053at2157; -.
DR   Proteomes; UP000001941; Chromosome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10050; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00100_A; IF_2_A; 1.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR029459; EFTU-type.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR004544; TF_aIF-2_arc.
DR   InterPro; IPR015760; TIF_IF2.
DR   InterPro; IPR023115; TIF_IF2_dom3.
DR   InterPro; IPR036925; TIF_IF2_dom3_sf.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   PANTHER; PTHR43381; PTHR43381; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF14578; GTP_EFTU_D4; 1.
DR   Pfam; PF11987; IF-2; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF52156; SSF52156; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00491; aIF-2; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Initiation factor; Nucleotide-binding; Protein biosynthesis;
KW   Reference proteome.
FT   CHAIN           1..604
FT                   /note="Probable translation initiation factor IF-2"
FT                   /id="PRO_0000335531"
FT   DOMAIN          18..232
FT                   /note="tr-type G"
FT   REGION          27..34
FT                   /note="G1"
FT                   /evidence="ECO:0000250"
FT   REGION          52..56
FT                   /note="G2"
FT                   /evidence="ECO:0000250"
FT   REGION          88..91
FT                   /note="G3"
FT                   /evidence="ECO:0000250"
FT   REGION          142..145
FT                   /note="G4"
FT                   /evidence="ECO:0000250"
FT   REGION          210..212
FT                   /note="G5"
FT                   /evidence="ECO:0000250"
FT   BINDING         27..34
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         88..92
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         142..145
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ   SEQUENCE   604 AA;  66261 MW;  2E38EEF0F9C850C9 CRC64;
     MGKKEVKKNK NEGDTPHIRT PIVCVLGHVD HGKTSLLDRI RGSSVVAGEA GAITQHIGAT
     IVPIDSIMSM SGGMKNLNIS IPGLLFIDTP GHHAFTTLRA RGGALADMAI VVVDITEGFQ
     PQTIEAIQIL RNCKTPFVVA ATKLDRIPGW RSTEKSPFLK SFAAQNERVT LLVETKVYDI
     VATLAEHGFS SERFDRVSDF ARNLAIVPVS AHTGEGIPDL LMVLIGLAQR YMEEALTLTV
     DGPGSGTVLE VKEEKGLGAT IDVILFDGTI RVGDEIAIAT TEGVQTTKVR SLLQPRPMQE
     ILVEDRFLRV KEVVAAAGVK VSAPGLDQVI AGSPVRVIGE DPEEVKTSIA KEMTEINVSL
     SPEGLIIKAD TIGALEALCK ELTAHEIPVM RAEVGQVSRH DVIEAETIKD PLYRVLIAFN
     TPVLPDAQDL LKEPAYAEMI QFFSGNVIYH ILEDYLEWRN NLKRIMDQKR FEKIIFPAKI
     LVLPGCVFRQ NNPAVVGVRI LSGTLRTGVN LVRRDGKKAG TLKSMQLRKE NIQEAHAGDE
     VAISIEGATV GRQFDVEDEL LVGIPERHVK VLETEMLSHL NEDAKEVLNE YTRLFRKENP
     FWGK