ID   IF2P_METKA              Reviewed;         598 AA.
AC   Q8TV06;
DT   02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Probable translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN   Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=MK1595;
OS   Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938).
OC   Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae;
OC   Methanopyrus.
OX   NCBI_TaxID=190192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX   PubMed=11930014; DOI=10.1073/pnas.032671499;
RA   Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N.,
RA   Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A.,
RA   Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G.,
RA   Koonin E.V., Kozyavkin S.A.;
RT   "The complete genome of hyperthermophile Methanopyrus kandleri AV19 and
RT   monophyly of archaeal methanogens.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
CC   -!- FUNCTION: Function in general translation initiation by promoting the
CC       binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC       along with eIF-2. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00100}.
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DR   EMBL; AE009439; AAM02808.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8TV06; -.
DR   SMR; Q8TV06; -.
DR   STRING; 190192.MK1595; -.
DR   PRIDE; Q8TV06; -.
DR   EnsemblBacteria; AAM02808; AAM02808; MK1595.
DR   KEGG; mka:MK1595; -.
DR   PATRIC; fig|190192.8.peg.1756; -.
DR   HOGENOM; CLU_002656_3_3_2; -.
DR   OMA; FRQSKPA; -.
DR   Proteomes; UP000001826; Chromosome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10050; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00100_A; IF_2_A; 1.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR029459; EFTU-type.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR004544; TF_aIF-2_arc.
DR   InterPro; IPR015760; TIF_IF2.
DR   InterPro; IPR023115; TIF_IF2_dom3.
DR   InterPro; IPR036925; TIF_IF2_dom3_sf.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   PANTHER; PTHR43381; PTHR43381; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF14578; GTP_EFTU_D4; 1.
DR   Pfam; PF11987; IF-2; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF52156; SSF52156; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00491; aIF-2; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Initiation factor; Nucleotide-binding; Protein biosynthesis;
KW   Reference proteome.
FT   CHAIN           1..598
FT                   /note="Probable translation initiation factor IF-2"
FT                   /id="PRO_0000137302"
FT   DOMAIN          8..226
FT                   /note="tr-type G"
FT   REGION          17..24
FT                   /note="G1"
FT                   /evidence="ECO:0000250"
FT   REGION          42..46
FT                   /note="G2"
FT                   /evidence="ECO:0000250"
FT   REGION          81..84
FT                   /note="G3"
FT                   /evidence="ECO:0000250"
FT   REGION          135..138
FT                   /note="G4"
FT                   /evidence="ECO:0000250"
FT   REGION          203..205
FT                   /note="G5"
FT                   /evidence="ECO:0000250"
FT   BINDING         17..24
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         81..85
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         135..138
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ   SEQUENCE   598 AA;  66605 MW;  080BB46FB486FB27 CRC64;
     MSESNKAIRQ PIISVLGHVD HGKTTLLDKI RGTAVAAKEA GGITQHIGAS EIPLEVVKEI
     CGPLLEQLDV EITIPGLLFI DTPGHEAFTN LRRRGGALAD IAILVIDIME GVMPQTEEAL
     RILRRYRTPF VVAANKVDRV PGWKSHEDTP FLESFQKQSP EVQQRLEEKV YELIGQLHQH
     GFQAERFDRV RDFTRTVAIV PTSGVTGEGI PELLMVVTGL AQRFLEEQLK IEVEGPGKAA
     ILEVKEEPGL GHTVDAILYD GIIRTGDTIV IGHPEEPIVT RVRSLLKPKP LDEMRDPSDR
     FRKVDEVTAA AGVKISAPEL EEAVAGAPLR VVGEDEDVEE VVREVQEEVE EVTIETDQEG
     IIIKADTLGT LEAVVGEFKE KDVPIRKADV GDITKKDVIE AHAVAEKDPL LGVIVGFNVG
     VTEEARELAD EYDVDIIIDD VIYELVEKYE EMVEKRIERE RRKRLDELVR PGKIKVLPGY
     IFRQSKPAIV GVQVLAGVIK PGYPLMREDG RELGEIKQIQ MHGEPIKEAK KGQEVAISIE
     GPIVGRHFEE GDILYTDVPS EHAKLMFEEF KDLLTEDELE ALKEIAEIKR KEDPFYGM