ID   IF2P_METLZ              Reviewed;         589 AA.
AC   A2STM8;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Probable translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN   Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=Mlab_1520;
OS   Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
OX   NCBI_TaxID=410358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43576 / DSM 4855 / Z;
RX   PubMed=21304657; DOI=10.4056/sigs.35575;
RA   Anderson I.J., Sieprawska-Lupa M., Goltsman E., Lapidus A., Copeland A.,
RA   Glavina Del Rio T., Tice H., Dalin E., Barry K., Pitluck S., Hauser L.,
RA   Land M., Lucas S., Richardson P., Whitman W.B., Kyrpides N.C.;
RT   "Complete genome sequence of Methanocorpusculum labreanum type strain Z.";
RL   Stand. Genomic Sci. 1:197-203(2009).
CC   -!- FUNCTION: Function in general translation initiation by promoting the
CC       binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC       along with eIF-2. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00100}.
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DR   EMBL; CP000559; ABN07684.1; -; Genomic_DNA.
DR   RefSeq; WP_011833887.1; NC_008942.1.
DR   AlphaFoldDB; A2STM8; -.
DR   SMR; A2STM8; -.
DR   STRING; 410358.Mlab_1520; -.
DR   PRIDE; A2STM8; -.
DR   EnsemblBacteria; ABN07684; ABN07684; Mlab_1520.
DR   GeneID; 4794572; -.
DR   KEGG; mla:Mlab_1520; -.
DR   eggNOG; arCOG01560; Archaea.
DR   HOGENOM; CLU_002656_3_3_2; -.
DR   OMA; FRQSKPA; -.
DR   OrthoDB; 17053at2157; -.
DR   Proteomes; UP000000365; Chromosome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10050; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00100_A; IF_2_A; 1.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR029459; EFTU-type.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR004544; TF_aIF-2_arc.
DR   InterPro; IPR015760; TIF_IF2.
DR   InterPro; IPR023115; TIF_IF2_dom3.
DR   InterPro; IPR036925; TIF_IF2_dom3_sf.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   PANTHER; PTHR43381; PTHR43381; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF14578; GTP_EFTU_D4; 1.
DR   Pfam; PF11987; IF-2; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF52156; SSF52156; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00491; aIF-2; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Initiation factor; Nucleotide-binding; Protein biosynthesis;
KW   Reference proteome.
FT   CHAIN           1..589
FT                   /note="Probable translation initiation factor IF-2"
FT                   /id="PRO_0000335527"
FT   DOMAIN          5..219
FT                   /note="tr-type G"
FT   REGION          14..21
FT                   /note="G1"
FT                   /evidence="ECO:0000250"
FT   REGION          39..43
FT                   /note="G2"
FT                   /evidence="ECO:0000250"
FT   REGION          75..78
FT                   /note="G3"
FT                   /evidence="ECO:0000250"
FT   REGION          129..132
FT                   /note="G4"
FT                   /evidence="ECO:0000250"
FT   REGION          197..199
FT                   /note="G5"
FT                   /evidence="ECO:0000250"
FT   BINDING         14..21
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         75..79
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         129..132
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ   SEQUENCE   589 AA;  64162 MW;  1BF268EBAA030696 CRC64;
     MEEHIRTPIV CVLGHVDHGK TSLLDRIRGS KVVAGEAGAI TQHIGATLIP FDSIAKMSGD
     LGRLKTSVPG LLFIDTPGHH AFTTLRARGG ALADIAILVV DVNEGFKQQT IEALQILRTC
     KTPFVIAATK LDKIPGWRPT PNASFQKAYK NQSERVQTEC ENRVYELVGK LSDMGFNSER
     FDRVSDFQRN LVIVPVSSMT GEGIGDLLMI MIGLAQRYLT EGLKTTTSGP GVGTVLEVKE
     EKGLGTTLDV ILYDGIISVG DEIGIAGSDG ALSTKVRALL QPRPMKEILI EDQFMRVKSV
     VAAAGVKISA PNLESIVAGS PIRVIRGDHD EVLAKINEEM QEINIKLSDV GVSVRADTIG
     ALEALSNELD AKNIPIMRAS VGPLSRRDLI DISVIKEDLF KVALCFNVPL LPDAEAMVRD
     EEVDVKIFSN RVIYKLLDDY LEWRDEMIRA KEAKQFETVV LPAKFSILPG CVFRMSGPAV
     VGVRVLGGTL RPKVSIATRD GKIVGEIKQI KLNKETIGEA KEGAEVAVSI DGVTIGRQID
     VGETLYVAIP ERHVKVLETE MLSHLNAGTV EALEEYTGIF RKTQPFWGK