ID   IF2P_METMA              Reviewed;         591 AA.
AC   Q8PU78;
DT   10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   10-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 136.
DE   RecName: Full=Probable translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN   Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=MM_2463;
OS   Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM
OS   11833 / OCM 88) (Methanosarcina frisia).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=192952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=12125824;
RA   Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA   Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA   Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA   Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA   Fritz H.-J., Gottschalk G.;
RT   "The genome of Methanosarcina mazei: evidence for lateral gene transfer
RT   between Bacteria and Archaea.";
RL   J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
CC   -!- FUNCTION: Function in general translation initiation by promoting the
CC       binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC       along with eIF-2. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00100}.
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DR   EMBL; AE008384; AAM32159.1; -; Genomic_DNA.
DR   RefSeq; WP_011034381.1; NC_003901.1.
DR   AlphaFoldDB; Q8PU78; -.
DR   SMR; Q8PU78; -.
DR   STRING; 192952.MM_2463; -.
DR   EnsemblBacteria; AAM32159; AAM32159; MM_2463.
DR   GeneID; 24882185; -.
DR   KEGG; mma:MM_2463; -.
DR   PATRIC; fig|192952.21.peg.2818; -.
DR   eggNOG; arCOG01560; Archaea.
DR   HOGENOM; CLU_002656_3_3_2; -.
DR   OMA; FRQSKPA; -.
DR   Proteomes; UP000000595; Chromosome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10050; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00100_A; IF_2_A; 1.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR029459; EFTU-type.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR004544; TF_aIF-2_arc.
DR   InterPro; IPR015760; TIF_IF2.
DR   InterPro; IPR023115; TIF_IF2_dom3.
DR   InterPro; IPR036925; TIF_IF2_dom3_sf.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   PANTHER; PTHR43381; PTHR43381; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF14578; GTP_EFTU_D4; 1.
DR   Pfam; PF11987; IF-2; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF52156; SSF52156; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00491; aIF-2; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
DR   PROSITE; PS01176; IF2; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Initiation factor; Nucleotide-binding; Protein biosynthesis;
KW   Reference proteome.
FT   CHAIN           1..591
FT                   /note="Probable translation initiation factor IF-2"
FT                   /id="PRO_0000137303"
FT   DOMAIN          7..223
FT                   /note="tr-type G"
FT   REGION          16..23
FT                   /note="G1"
FT                   /evidence="ECO:0000250"
FT   REGION          41..45
FT                   /note="G2"
FT                   /evidence="ECO:0000250"
FT   REGION          78..81
FT                   /note="G3"
FT                   /evidence="ECO:0000250"
FT   REGION          132..135
FT                   /note="G4"
FT                   /evidence="ECO:0000250"
FT   REGION          200..202
FT                   /note="G5"
FT                   /evidence="ECO:0000250"
FT   BINDING         16..23
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         78..82
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         132..135
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ   SEQUENCE   591 AA;  64901 MW;  FE328265BBD887DF CRC64;
     MTDKKNLRTP IVCVMGHVDH GKTTLLDKIR GTAIVSGEAG AITQHIGATE VPIDVIIDKL
     GDPRLRDRFM VPGLLFIDTP GHHAFTTLRS RGGALADLAI VVVDINEGFK PQTYESLQIL
     KRFKTPFVVV ANKIDRIGGW VSQKDMPFAA TFKKQSSDVQ ARLETKLYEV IGELYNQGFA
     AERYDRVTNF QKTLGVVPVS AFTGEGIPDV LMVLLGLAQK FLEANLQYSA KGPGVGTVLE
     VKEEKGLGAT LDIILYDGTL KKGDTVVIGS LGEPIRTKVR ALLKPRELSE IRYESKFQQV
     SKVTAAAGVK ISAPGLEGAL AGSPIRVATE DNLEEIASQV KSEIDEVRID TGSVGIMIKA
     DTLGSLEALV HEFQKDEVPI RKAEVGDISH RDAVEASTVE DPLYSVIIGF NVKVHPDARD
     FLQESSVKVF TSDVIYRLVE DYQKYVKEQQ EKAEKKIFET IIRPGKFKIL PGCVFRQSKP
     AVVGVRVLGG VVRTNTDVML ENGNVVGKIK GLQSEGENIP SAKVGKEVAM AIEGATVGRQ
     IKEEDVLYVN VPERHAKVLE HEIYESLSTD EKETLDIFLT LKRKDNPFWA K