ID   IF2P_METMJ              Reviewed;         593 AA.
AC   A3CSP4;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Probable translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN   Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=Memar_0461;
OS   Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanomicrobiales; Methanomicrobiaceae; Methanoculleus.
OX   NCBI_TaxID=368407;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35101 / DSM 1498 / JR1;
RX   PubMed=21304656; DOI=10.4056/sigs.32535;
RA   Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A.,
RA   Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C.,
RA   Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S., Hauser L.,
RA   Land M., Lucas S., Richardson P., Whitman W.B., Kyrpides N.C.;
RT   "Complete genome sequence of Methanoculleus marisnigri Romesser et al. 1981
RT   type strain JR1.";
RL   Stand. Genomic Sci. 1:189-196(2009).
CC   -!- FUNCTION: Function in general translation initiation by promoting the
CC       binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC       along with eIF-2. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00100}.
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DR   EMBL; CP000562; ABN56394.1; -; Genomic_DNA.
DR   RefSeq; WP_011843304.1; NC_009051.1.
DR   AlphaFoldDB; A3CSP4; -.
DR   SMR; A3CSP4; -.
DR   STRING; 368407.Memar_0461; -.
DR   PRIDE; A3CSP4; -.
DR   EnsemblBacteria; ABN56394; ABN56394; Memar_0461.
DR   GeneID; 4846305; -.
DR   KEGG; mem:Memar_0461; -.
DR   eggNOG; arCOG01560; Archaea.
DR   HOGENOM; CLU_002656_3_3_2; -.
DR   OMA; FRQSKPA; -.
DR   OrthoDB; 17053at2157; -.
DR   Proteomes; UP000002146; Chromosome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10050; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00100_A; IF_2_A; 1.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR029459; EFTU-type.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR004544; TF_aIF-2_arc.
DR   InterPro; IPR015760; TIF_IF2.
DR   InterPro; IPR023115; TIF_IF2_dom3.
DR   InterPro; IPR036925; TIF_IF2_dom3_sf.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   PANTHER; PTHR43381; PTHR43381; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF14578; GTP_EFTU_D4; 1.
DR   Pfam; PF11987; IF-2; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF52156; SSF52156; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00491; aIF-2; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Initiation factor; Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..593
FT                   /note="Probable translation initiation factor IF-2"
FT                   /id="PRO_1000057658"
FT   DOMAIN          7..221
FT                   /note="tr-type G"
FT   REGION          16..23
FT                   /note="G1"
FT                   /evidence="ECO:0000250"
FT   REGION          41..45
FT                   /note="G2"
FT                   /evidence="ECO:0000250"
FT   REGION          77..80
FT                   /note="G3"
FT                   /evidence="ECO:0000250"
FT   REGION          131..134
FT                   /note="G4"
FT                   /evidence="ECO:0000250"
FT   REGION          199..201
FT                   /note="G5"
FT                   /evidence="ECO:0000250"
FT   BINDING         16..23
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         77..81
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         131..134
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ   SEQUENCE   593 AA;  65159 MW;  3E555D64DC1926B8 CRC64;
     MAKESTIRTP IVCVMGHVDH GKTSLLDRIR GSSVVSTEEG EITQHIGATL VPIDAVTRMG
     GALSKVSVNV PGLLFIDTPG HHAFTTLRAR GGALADMAIV VVDINEGFRP QTIEALQILR
     NYKTPFVIAA NKVDRIHGWR VQENQPFLKT FAQQNERVQG MVETKVYELV GKLSDLGFNS
     ERFDRVSDFA RNICIVPTSA LTGEGLPDIL MVLIGLAQRY MTESLKVSAD GPGAGTVLEV
     KEERGLGMTL DLILYDGTLK VGDEIVVAGN DQIIETKVRS LLKPRPMSEI LIEERFERVK
     SVTAAAGIKV AAPKLDGVIA GSPLRAVRSG NRDEVIEQVR REVQDIEVNL SDVGVIIRAD
     TIGALEALSK ELEGHQIQVM RATVGPVTRH DVIEAGTIKD PLYSAIIAFN TPVLPDAVDA
     LADTAMSHVS IFEGGVIYQL IDDYVEWRDE KKQELERQKF EKLIMPAKIR ILPNCVFRQS
     NPAVVGVRIL GGKLQSGVDL ALPNGKKIGR IKQIQAKNET VQEAEAGKEV AISIEGPTVG
     RQINVDDDLY VDIPERHVKV IEREVIDHLS PSLRETLEEF TTLKRREDPF WGK