ID   IF2P_METS5              Reviewed;         597 AA.
AC   A4YCQ5;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Probable translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN   Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=Msed_0030;
OS   Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509
OS   / TH2).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Metallosphaera.
OX   NCBI_TaxID=399549;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2;
RX   PubMed=18083856; DOI=10.1128/aem.02019-07;
RA   Auernik K.S., Maezato Y., Blum P.H., Kelly R.M.;
RT   "The genome sequence of the metal-mobilizing, extremely thermoacidophilic
RT   archaeon Metallosphaera sedula provides insights into bioleaching-
RT   associated metabolism.";
RL   Appl. Environ. Microbiol. 74:682-692(2008).
CC   -!- FUNCTION: Function in general translation initiation by promoting the
CC       binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC       along with eIF-2. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00100}.
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DR   EMBL; CP000682; ABP94207.1; -; Genomic_DNA.
DR   RefSeq; WP_011921176.1; NC_009440.1.
DR   AlphaFoldDB; A4YCQ5; -.
DR   SMR; A4YCQ5; -.
DR   STRING; 399549.Msed_0030; -.
DR   EnsemblBacteria; ABP94207; ABP94207; Msed_0030.
DR   GeneID; 5105169; -.
DR   GeneID; 59456538; -.
DR   KEGG; mse:Msed_0030; -.
DR   eggNOG; arCOG01560; Archaea.
DR   HOGENOM; CLU_002656_3_3_2; -.
DR   OMA; FRQSKPA; -.
DR   Proteomes; UP000000242; Chromosome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10050; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00100_A; IF_2_A; 1.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR029459; EFTU-type.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR004544; TF_aIF-2_arc.
DR   InterPro; IPR015760; TIF_IF2.
DR   InterPro; IPR023115; TIF_IF2_dom3.
DR   InterPro; IPR036925; TIF_IF2_dom3_sf.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   PANTHER; PTHR43381; PTHR43381; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF14578; GTP_EFTU_D4; 1.
DR   Pfam; PF11987; IF-2; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF52156; SSF52156; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00491; aIF-2; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Initiation factor; Nucleotide-binding; Protein biosynthesis;
KW   Reference proteome.
FT   CHAIN           1..597
FT                   /note="Probable translation initiation factor IF-2"
FT                   /id="PRO_0000335525"
FT   DOMAIN          8..225
FT                   /note="tr-type G"
FT   REGION          17..24
FT                   /note="G1"
FT                   /evidence="ECO:0000250"
FT   REGION          42..46
FT                   /note="G2"
FT                   /evidence="ECO:0000250"
FT   REGION          81..84
FT                   /note="G3"
FT                   /evidence="ECO:0000250"
FT   REGION          135..138
FT                   /note="G4"
FT                   /evidence="ECO:0000250"
FT   REGION          203..205
FT                   /note="G5"
FT                   /evidence="ECO:0000250"
FT   BINDING         17..24
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         81..85
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         135..138
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ   SEQUENCE   597 AA;  66239 MW;  78135CCE538D0BE1 CRC64;
     MSKTEVRLRQ PIVVVLGHVD HGKTTLLDKI RGTAVVKKEP GEMTQEVGAS FVPSSVIEKV
     AQPLKGIIPV KLEIPGLLFI DTPGHELFSN LRRRGGSVAD IAILVVDITE GFQKQTIESI
     EILKDKKVPF LVAANKIDRI PGWRPIENET FTKSFRSQSQ SVQKALDNYV YKLVMQLAEM
     GFNAERFDRV RDFTRYVSIV PVSGKTGEGL PELLALLAGL TQQYLKTRLR AVEGPAKGVI
     LEVKEEQGLG HTIDVIIYDG ILRKSDTIVL GGINGVIVTK IRNILLPAPL QDMRMTRSGY
     RSIDEISAAA GVKISAPGLE EALAGSPLYV ANDEAKLQEA RKLIEEELSK VRFSKNSTGI
     VVKADSLGSL ESLVAGFEKI GIPVRVADIG PITKRDLIEA ELSAKEVEEY GIIAAFRVKP
     IPGLDTSKVK IIYNDIIYQL IDDTIKYIED LRERRKRRTL DSLVLPGKIK ILPGYVFRRS
     DPAIVGVEVL GGVVRPKYPL IREDGQRVGE VLAIQDNKKN IERATKGMEV SMSIKGNIMI
     GRQVQEGDVL YTDVPQEDLE ILFKNYKDSI TEDMMEVIKE LIKIKKAENP LYGIFIQ