ID   IF2P_METST              Reviewed;         613 AA.
AC   Q2NGM6;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Probable translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN   Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=Msp_0629;
OS   Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 /
OS   MCB-3).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanosphaera.
OX   NCBI_TaxID=339860;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3;
RX   PubMed=16385054; DOI=10.1128/jb.188.2.642-658.2006;
RA   Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R.,
RA   Gottschalk G., Thauer R.K.;
RT   "The genome sequence of Methanosphaera stadtmanae reveals why this human
RT   intestinal archaeon is restricted to methanol and H2 for methane formation
RT   and ATP synthesis.";
RL   J. Bacteriol. 188:642-658(2006).
CC   -!- FUNCTION: Function in general translation initiation by promoting the
CC       binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC       along with eIF-2. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00100}.
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DR   EMBL; CP000102; ABC57027.1; -; Genomic_DNA.
DR   RefSeq; WP_011406227.1; NC_007681.1.
DR   AlphaFoldDB; Q2NGM6; -.
DR   SMR; Q2NGM6; -.
DR   STRING; 339860.Msp_0629; -.
DR   EnsemblBacteria; ABC57027; ABC57027; Msp_0629.
DR   GeneID; 41325205; -.
DR   KEGG; mst:Msp_0629; -.
DR   eggNOG; arCOG01560; Archaea.
DR   HOGENOM; CLU_002656_3_3_2; -.
DR   OMA; FRQSKPA; -.
DR   OrthoDB; 17053at2157; -.
DR   Proteomes; UP000001931; Chromosome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10050; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00100_A; IF_2_A; 1.
DR   InterPro; IPR029459; EFTU-type.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR004544; TF_aIF-2_arc.
DR   InterPro; IPR015760; TIF_IF2.
DR   InterPro; IPR023115; TIF_IF2_dom3.
DR   InterPro; IPR036925; TIF_IF2_dom3_sf.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   PANTHER; PTHR43381; PTHR43381; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF14578; GTP_EFTU_D4; 1.
DR   Pfam; PF11987; IF-2; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF52156; SSF52156; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00491; aIF-2; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Initiation factor; Nucleotide-binding; Protein biosynthesis;
KW   Reference proteome.
FT   CHAIN           1..613
FT                   /note="Probable translation initiation factor IF-2"
FT                   /id="PRO_0000335530"
FT   DOMAIN          3..220
FT                   /note="tr-type G"
FT   REGION          12..19
FT                   /note="G1"
FT                   /evidence="ECO:0000250"
FT   REGION          37..41
FT                   /note="G2"
FT                   /evidence="ECO:0000250"
FT   REGION          76..79
FT                   /note="G3"
FT                   /evidence="ECO:0000250"
FT   REGION          130..133
FT                   /note="G4"
FT                   /evidence="ECO:0000250"
FT   REGION          198..200
FT                   /note="G5"
FT                   /evidence="ECO:0000250"
FT   BINDING         12..19
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         76..80
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         130..133
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ   SEQUENCE   613 AA;  68340 MW;  2F8B2ECAC877B9F6 CRC64;
     MKTRSPIVSV LGHVDHGKTT LLDHIRGSTI ASKEAGGITQ HIGATEIPMD VISSICGGFL
     EKMNIQEQLP GLFFIDTPGH EAFTTLRKRG GSLADLAILI MDVTEGFKPQ TYEALNILKS
     SKTPFVVAAN KIDKIPGWNS TKGECFSKAV QNQHKNVVFD LDQKIYEIVG TLHEEGFESE
     RFDRVSNFAS QITIVPISAY TGEGLPELLT MLLGLAYQYL NEQLQIEEDA PASGTVLEVK
     EEKGLGLTID TILYDGVLNK DDRIMMLTKE NKVISTKIRS LLKPKPLEEI RESKTMFEDT
     NQIVAAAGVK IVAPHVDDVV SGSPLKVAND DNIRVEDELL SEVDNIRIQT NDIGILVKAD
     TLGSLEALVN ILDSKDIPIK SAEIGDISRR DIINASIMYE EDEKYGVIIA FNVNILPSAE
     DELNDQNIMV FQDRVIYQLT EDYLNWVNSA KERQKKAKLA SIIRPSKIRI MPKLVFRHSK
     PAIAGVEIMS GIIEKGVTLI NDKGHVVGRV ESMEDNGENL PKVSRGSQVA MAIGDAVFEK
     DFEEGDVLYV DMSERNFFAI NNELKDKLLD DEILTMEELQ EIKQETEDSN WGVINTDWSE
     LDTLDEDEYE DFY