IF2P_METTH
ID IF2P_METTH Reviewed; 594 AA.
AC O26359;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Probable translation initiation factor IF-2;
GN Name=infB; OrderedLocusNames=MTH_259;
OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
CC -!- FUNCTION: Function in general translation initiation by promoting the
CC binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC along with eIF-2 (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000305}.
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DR EMBL; AE000666; AAB84765.1; -; Genomic_DNA.
DR PIR; E69132; E69132.
DR RefSeq; WP_010875898.1; NC_000916.1.
DR PDB; 1G7R; X-ray; 2.20 A; A=1-594.
DR PDB; 1G7S; X-ray; 2.00 A; A=1-594.
DR PDB; 1G7T; X-ray; 2.00 A; A=1-594.
DR PDB; 4V8Z; EM; 6.60 A; CV=351-586.
DR PDBsum; 1G7R; -.
DR PDBsum; 1G7S; -.
DR PDBsum; 1G7T; -.
DR PDBsum; 4V8Z; -.
DR AlphaFoldDB; O26359; -.
DR SMR; O26359; -.
DR STRING; 187420.MTH_259; -.
DR EnsemblBacteria; AAB84765; AAB84765; MTH_259.
DR GeneID; 1470220; -.
DR KEGG; mth:MTH_259; -.
DR PATRIC; fig|187420.15.peg.228; -.
DR HOGENOM; CLU_002656_3_3_2; -.
DR OMA; FRQSKPA; -.
DR EvolutionaryTrace; O26359; -.
DR Proteomes; UP000005223; Chromosome.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.10050; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00100_A; IF_2_A; 1.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR029459; EFTU-type.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR004544; TF_aIF-2_arc.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR43381; PTHR43381; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF14578; GTP_EFTU_D4; 1.
DR Pfam; PF11987; IF-2; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF52156; SSF52156; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00491; aIF-2; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; GTP-binding; Initiation factor; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome.
FT CHAIN 1..594
FT /note="Probable translation initiation factor IF-2"
FT /id="PRO_0000137305"
FT DOMAIN 3..220
FT /note="tr-type G"
FT REGION 12..19
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 37..41
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 76..79
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 130..133
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 198..200
FT /note="G5"
FT /evidence="ECO:0000250"
FT BINDING 12..19
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 76..80
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 130..133
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT STRAND 7..11
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 18..30
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 44..48
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 49..55
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 58..62
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 64..67
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 71..75
FT /evidence="ECO:0007829|PDB:1G7S"
FT TURN 79..81
FT /evidence="ECO:0007829|PDB:1G7T"
FT HELIX 83..85
FT /evidence="ECO:0007829|PDB:1G7T"
FT STRAND 86..88
FT /evidence="ECO:0007829|PDB:1G7T"
FT STRAND 89..93
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 95..102
FT /evidence="ECO:0007829|PDB:1G7S"
FT TURN 103..105
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 109..120
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 125..130
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 132..134
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 146..150
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 155..174
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 178..181
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 182..184
FT /evidence="ECO:0007829|PDB:1G7S"
FT TURN 188..190
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 191..196
FT /evidence="ECO:0007829|PDB:1G7S"
FT TURN 199..201
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 205..219
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 222..224
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 233..242
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 245..259
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 263..276
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 279..283
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 295..298
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 300..311
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 316..318
FT /evidence="ECO:0007829|PDB:1G7T"
FT STRAND 323..326
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 330..342
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 349..351
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 354..359
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 360..372
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 378..385
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 387..397
FT /evidence="ECO:0007829|PDB:1G7S"
FT TURN 401..403
FT /evidence="ECO:0007829|PDB:1G7R"
FT STRAND 405..410
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 415..420
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 422..432
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 434..457
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 464..475
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 477..492
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 496..498
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 504..513
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 516..522
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 527..532
FT /evidence="ECO:0007829|PDB:1G7S"
FT TURN 537..539
FT /evidence="ECO:0007829|PDB:1G7S"
FT STRAND 545..548
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 552..556
FT /evidence="ECO:0007829|PDB:1G7S"
FT HELIX 568..581
FT /evidence="ECO:0007829|PDB:1G7S"
SQ SEQUENCE 594 AA; 66249 MW; B2EDA07718DEBE02 CRC64;
MKIRSPIVSV LGHVDHGKTT LLDHIRGSAV ASREAGGITQ HIGATEIPMD VIEGICGDFL
KKFSIRETLP GLFFIDTPGH EAFTTLRKRG GALADLAILI VDINEGFKPQ TQEALNILRM
YRTPFVVAAN KIDRIHGWRV HEGRPFMETF SKQDIQVQQK LDTKVYELVG KLHEEGFESE
RFDRVTDFAS QVSIIPISAI TGEGIPELLT MLMGLAQQYL REQLKIEEDS PARGTILEVK
EETGLGMTID AVIYDGILRK DDTIAMMTSK DVISTRIRSL LKPRPLEEMR ESRKKFQKVD
EVVAAAGIKI VAPGIDDVMA GSPLRVVTDP EKVREEILSE IEDIKIDTDE AGVVVKADTL
GSLEAVVKIL RDMYVPIKVA DIGDVSRRDV VNAGIALQED RVYGAIIAFN VKVIPSAAQE
LKNSDIKLFQ GNVIYRLMEE YEEWVRGIEE EKKKKWMEAI IKPASIRLIP KLVFRQSKPA
IGGVEVLTGV IRQGYPLMND DGETVGTVES MQDKGENLKS ASRGQKVAMA IKDAVYGKTI
HEGDTLYVDI PENHYHILKE QLSGDLTDEE LDLMDKIAEI KRKKNPDWGM KAPF
