ID   IF2P_METTP              Reviewed;         602 AA.
AC   A0B8Q6;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Probable translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN   Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=Mthe_1302;
OS   Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 /
OS   PT) (Methanosaeta thermophila).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanotrichales; Methanotrichaceae; Methanothrix.
OX   NCBI_TaxID=349307;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 6194 / JCM 14653 / NBRC 101360 / PT;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P.,
RA   Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Kim E., Smith K.S., Ingram-Smith C., Richardson P.;
RT   "Complete sequence of Methanosaeta thermophila PT.";
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Function in general translation initiation by promoting the
CC       binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC       along with eIF-2. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000477; ABK15080.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0B8Q6; -.
DR   SMR; A0B8Q6; -.
DR   STRING; 349307.Mthe_1302; -.
DR   EnsemblBacteria; ABK15080; ABK15080; Mthe_1302.
DR   KEGG; mtp:Mthe_1302; -.
DR   HOGENOM; CLU_002656_3_3_2; -.
DR   OMA; FRQSKPA; -.
DR   Proteomes; UP000000674; Chromosome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10050; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00100_A; IF_2_A; 1.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR029459; EFTU-type.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR004544; TF_aIF-2_arc.
DR   InterPro; IPR015760; TIF_IF2.
DR   InterPro; IPR023115; TIF_IF2_dom3.
DR   InterPro; IPR036925; TIF_IF2_dom3_sf.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   PANTHER; PTHR43381; PTHR43381; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF14578; GTP_EFTU_D4; 1.
DR   Pfam; PF11987; IF-2; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF52156; SSF52156; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00491; aIF-2; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Initiation factor; Nucleotide-binding; Protein biosynthesis;
KW   Reference proteome.
FT   CHAIN           1..602
FT                   /note="Probable translation initiation factor IF-2"
FT                   /id="PRO_0000335529"
FT   DOMAIN          18..233
FT                   /note="tr-type G"
FT   REGION          27..34
FT                   /note="G1"
FT                   /evidence="ECO:0000250"
FT   REGION          52..56
FT                   /note="G2"
FT                   /evidence="ECO:0000250"
FT   REGION          88..91
FT                   /note="G3"
FT                   /evidence="ECO:0000250"
FT   REGION          142..145
FT                   /note="G4"
FT                   /evidence="ECO:0000250"
FT   REGION          210..212
FT                   /note="G5"
FT                   /evidence="ECO:0000250"
FT   BINDING         27..34
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         88..92
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         142..145
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ   SEQUENCE   602 AA;  66446 MW;  810C1EEC4E1F7EBC CRC64;
     MRQRSKKGMR SKEEVSQLRT PIVCVMGHVD HGKTTLLDRI RGTTVAQYEA GAITQHIGAT
     EIPLSVIQQF CGSGFKANLM VPGLLFIDTP GHHAFTSLRS RGGSLADLAI LIVDINEGFQ
     PQTIESINIL KRFKTPFVVA ANKIDRIPGW RPVENAPMEK SLAGQTERVV ETLETKIYEL
     VGELYKYGFD SNRYDRIADF TKTVGIIPVS AITGEGIPDL LLVLVGLAQR FLKQNLVIES
     SRPGMGTILE VKEERGLGTT LDVILYDGMI SVGDTIVVGT PREPIITKVR ALLKPRPLKE
     IRSEERFTPV KHVVAASGIK VSAPKLETAL AGSTIRVVGE GEDPEAIAKE IRSEIEAVRI
     DTDTVGVILK ADTIGSLEGL VGELRAKNIP IHVADVGPVT RRDVIRAAAI KDPLLSVILG
     FNVEILPDAL SEIQKLDIPV FQSDVIYTLL ESYEEWMEEK KMQMEQERLE AIVKPGCVRI
     LPDCVFRQSK PAIVGVQVVG GTISHNVPLI REDGAVVGTI RGIQQRNENI PMATVGQEVA
     ISIDGPTVGR QIHEGDLLYV NIPEKHARII EQELKQKMSQ DEIEVFEKFL EIKRKKDMFW
     GR