ID   IF2P_NATPD              Reviewed;         602 AA.
AC   Q3IMS5;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Probable translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN   Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=NP_4982A;
OS   Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM
OS   8858 / NBRC 14720 / NCIMB 2260 / Gabara) (Halobacterium pharaonis).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Haloarculaceae; Natronomonas.
OX   NCBI_TaxID=348780;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB
RC   2260 / Gabara;
RX   PubMed=16169924; DOI=10.1101/gr.3952905;
RA   Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J.,
RA   Oesterhelt D.;
RT   "Living with two extremes: conclusions from the genome sequence of
RT   Natronomonas pharaonis.";
RL   Genome Res. 15:1336-1343(2005).
CC   -!- FUNCTION: Function in general translation initiation by promoting the
CC       binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC       along with eIF-2. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CR936257; CAI50582.1; -; Genomic_DNA.
DR   RefSeq; WP_011324193.1; NC_007426.1.
DR   AlphaFoldDB; Q3IMS5; -.
DR   SMR; Q3IMS5; -.
DR   STRING; 348780.NP_4982A; -.
DR   PRIDE; Q3IMS5; -.
DR   EnsemblBacteria; CAI50582; CAI50582; NP_4982A.
DR   GeneID; 3703271; -.
DR   KEGG; nph:NP_4982A; -.
DR   eggNOG; arCOG01560; Archaea.
DR   HOGENOM; CLU_002656_3_3_2; -.
DR   OMA; FRQSKPA; -.
DR   OrthoDB; 17053at2157; -.
DR   Proteomes; UP000002698; Chromosome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10050; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00100_A; IF_2_A; 1.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR029459; EFTU-type.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR004544; TF_aIF-2_arc.
DR   InterPro; IPR015760; TIF_IF2.
DR   InterPro; IPR023115; TIF_IF2_dom3.
DR   InterPro; IPR036925; TIF_IF2_dom3_sf.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   PANTHER; PTHR43381; PTHR43381; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF14578; GTP_EFTU_D4; 1.
DR   Pfam; PF11987; IF-2; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF52156; SSF52156; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00491; aIF-2; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Initiation factor; Nucleotide-binding; Protein biosynthesis;
KW   Reference proteome.
FT   CHAIN           1..602
FT                   /note="Probable translation initiation factor IF-2"
FT                   /id="PRO_0000228267"
FT   DOMAIN          15..230
FT                   /note="tr-type G"
FT   REGION          24..31
FT                   /note="G1"
FT                   /evidence="ECO:0000250"
FT   REGION          49..53
FT                   /note="G2"
FT                   /evidence="ECO:0000250"
FT   REGION          86..89
FT                   /note="G3"
FT                   /evidence="ECO:0000250"
FT   REGION          140..143
FT                   /note="G4"
FT                   /evidence="ECO:0000250"
FT   REGION          208..210
FT                   /note="G5"
FT                   /evidence="ECO:0000250"
FT   BINDING         24..31
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         86..90
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         140..143
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ   SEQUENCE   602 AA;  65542 MW;  1D2433DC192017EC CRC64;
     MSESDTTDAG DGTALRTPIV AVLGHVDHGK TSLLDEVRGS AVTAGEAGAI TQHIGATAVP
     LDTISELAGQ LVSPEDFDLP GLLFIDTPGH HSFSTLRSRG GALADIAILV VDVNDGFQPQ
     SYEALDILKR TQTPFIVAAN KIDTVPGWNP NPDEPVQRTL EAQSDRAESR LNEQLYEIIG
     ELSDEGFSAD MYWRVQNFRE NIGVVPVSAE TGEGVPDLLT VLMGLSQRYL KEEMSIDVGG
     PGVGTVLEVK EERGFGTTLD IVLYDGTIRA DDTIVVGGKN ETIVTDVRAL LQPQPLAEIR
     TEKQFEQVEA VGAAAGIKIA APDLDDAMAG APVRVVRDRP VEEVIAEVEA ELADIQVVTE
     EEGIVVKADT LGSLEAIAAA LEEAEIPIVR AEVGDVAPRD IAVASTAEEP KHEAVLAFNV
     DVLDDAEREA EEKDVKLFAD DVIYQLVEEY DDYVTEIEEA QQEQILDKIE RPCRFRVLKD
     HVFRQSNPAV VGVEVLSGTL KRNSRVVKWD GNEPERVGEL KSLQDAGDDI DEARTGEQVA
     ASIDGPTVGR QIEEGDELWA EVPEKHAKIL EQELADEIPT DELEALRMYL DKQRKRDPFW
     GK