IF2P_PYRAB
ID IF2P_PYRAB Reviewed; 992 AA.
AC Q9UZK7; G8ZKB1;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Probable translation initiation factor IF-2;
DE Contains:
DE RecName: Full=Pab infB intein;
DE AltName: Full=Pab IF2 intein;
GN Name=infB; OrderedLocusNames=PYRAB11390; ORFNames=PAB0755;
OS Pyrococcus abyssi (strain GE5 / Orsay).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=272844;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GE5 / Orsay;
RX PubMed=12622808; DOI=10.1046/j.1365-2958.2003.03381.x;
RA Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O.,
RA Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J.,
RA Weissenbach J., Zivanovic Y., Forterre P.;
RT "An integrated analysis of the genome of the hyperthermophilic archaeon
RT Pyrococcus abyssi.";
RL Mol. Microbiol. 47:1495-1512(2003).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=GE5 / Orsay;
RX PubMed=22057919; DOI=10.1007/s00284-011-0035-x;
RA Gao J., Wang J.;
RT "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and
RT Pyrococcus furiosus DSM 3638.";
RL Curr. Microbiol. 64:118-129(2012).
CC -!- FUNCTION: Function in general translation initiation by promoting the
CC binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC along with eIF-2 (By similarity). {ECO:0000250}.
CC -!- PTM: This protein undergoes a protein self splicing that involves a
CC post-translational excision of the intervening region (intein) followed
CC by peptide ligation. {ECO:0000305}.
CC -!- MISCELLANEOUS: The intein interrupts the GTP-binding site.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000305}.
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DR EMBL; AJ248286; CAB50050.1; -; Genomic_DNA.
DR EMBL; HE613800; CCE70554.1; -; Genomic_DNA.
DR PIR; E75093; E75093.
DR AlphaFoldDB; Q9UZK7; -.
DR SMR; Q9UZK7; -.
DR STRING; 272844.PAB0755; -.
DR MEROPS; N10.004; -.
DR EnsemblBacteria; CAB50050; CAB50050; PAB0755.
DR KEGG; pab:PAB0755; -.
DR PATRIC; fig|272844.11.peg.1195; -.
DR eggNOG; arCOG01560; Archaea.
DR eggNOG; arCOG03151; Archaea.
DR HOGENOM; CLU_002656_3_4_2; -.
DR OMA; DHGKCLL; -.
DR PhylomeDB; Q9UZK7; -.
DR Proteomes; UP000000810; Chromosome.
DR Proteomes; UP000009139; Chromosome.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR Gene3D; 3.10.28.10; -; 1.
DR Gene3D; 3.40.50.10050; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00100_A; IF_2_A; 1.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR029459; EFTU-type.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR004544; TF_aIF-2_arc.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR43381; PTHR43381; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF14578; GTP_EFTU_D4; 1.
DR Pfam; PF11987; IF-2; 1.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF51294; SSF51294; 1.
DR SUPFAM; SSF52156; SSF52156; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00491; aIF-2; 1.
DR TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51722; G_TR_2; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 3: Inferred from homology;
KW Autocatalytic cleavage; Endonuclease; GTP-binding; Hydrolase;
KW Initiation factor; Intron homing; Nuclease; Nucleotide-binding;
KW Protein biosynthesis; Protein splicing.
FT CHAIN 1..20
FT /note="Probable translation initiation factor IF-2, 1st
FT part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000014487"
FT CHAIN 21..414
FT /note="Pab infB intein"
FT /evidence="ECO:0000255"
FT /id="PRO_0000014488"
FT CHAIN 415..992
FT /note="Probable translation initiation factor IF-2, 2nd
FT part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000014489"
FT DOMAIN 96..220
FT /note="DOD-type homing endonuclease"
FT DOMAIN 399..616
FT /note="tr-type G"
FT BINDING 472..476
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 526..529
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
SQ SEQUENCE 992 AA; 112225 MW; 9838BADA50E6F1C6 CRC64;
MTKRIRQPII AVLGHVDHGK CLLPDEKVVV PSVGFVTLKE LFETASKVVE RDDEKEIREL
DERITSVNGD GKTGLVKASY VWKVRHKGKV IRVKLKNWHG VTVTPEHPFL TTKGWKRADQ
LRPGDYVAVP RFIHGNEDEK IFLSYVKVKK SGEEWKEYFY LAGRKGNIDV NLLFVAPKRY
VVEFLRGYFE ERSEVKGESV IVEARELVEP LSLALLRFGI FSKIQGSKLI VTGKRNLEAF
KDYIGFKDER EKALEEAIEK VKGSEVYPIF EEIRRLRLLF GFTREELGSY AKYENSEAPT
YEELMEILDF IERGSPSLSK KIAILEGKLK AELRVLEEEG LIKDGKLTPL GRELLEVWRN
REFDSKDVDY IRNIAETLVF IPVENVEEEE YDGYVYDLTT ETHNFIANGI LVHNTTLLDR
IRKTNVAAKE AGGITQHIGA TEVPIEVVKK IAGPLIKLWK AEIKLPGLLF IDTPGHEAFT
SLRARGGSLA DLAVLVVDIN EGFQPQTIES IEILRKYRTP FVVAANKIDR IKGWVIEEDE
PFLMNIKKQD QRAVQELETK LWELIGKFYE FGFQANRFDR VQNFTRELAI VPISAKYGIG
IAELLVLIAG LSQRYLEEKL KIEVEGPARG TILEVREEPG LGHTIDVIIY DGTLHKDDTI
VVGGKDKAIV TKIRALLKPK PLDEIRDPRF RFDYVDEVTA AAGVKIAAPG LEEALAGSPV
IAAPTPEDVE KAKQEILEQI ERVVISTDKV GVIVKADTLG SLEALSKELQ EKEIPIRKAD
VGNVSKTDVM EALSVKEEEP KYGVILGFNV KVNEDAEEVA KAKDVKIFVG NVIYKLIEDY
EEWVKEEEEK KKRELLSKVT FPGVIRLYPD ERYVFRRSNP AIVGIEVIEG RIKPGVTLIK
QNGQKVGVIR SIKSRDEFLQ EAKKGQAVAI AIEGAIVGRH IHPGETLYVD LSRDDAITLL
KHLRDTLEDT DIKALKMIAK VKAKEDPFWR AI
