ID   IF2P_PYRAE              Reviewed;         589 AA.
AC   Q8ZX20;
DT   05-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   05-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Probable translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN   Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=PAE1513;
OS   Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP
OS   104966 / NBRC 100827 / IM2).
OC   Archaea; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC   Pyrobaculum.
OX   NCBI_TaxID=178306;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2;
RX   PubMed=11792869; DOI=10.1073/pnas.241636498;
RA   Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I.,
RA   Miller J.H.;
RT   "Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum
RT   aerophilum.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002).
CC   -!- FUNCTION: Function in general translation initiation by promoting the
CC       binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC       along with eIF-2. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE009441; AAL63529.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8ZX20; -.
DR   SMR; Q8ZX20; -.
DR   STRING; 178306.PAE1513; -.
DR   EnsemblBacteria; AAL63529; AAL63529; PAE1513.
DR   KEGG; pai:PAE1513; -.
DR   PATRIC; fig|178306.9.peg.1116; -.
DR   eggNOG; arCOG01560; Archaea.
DR   HOGENOM; CLU_002656_3_3_2; -.
DR   InParanoid; Q8ZX20; -.
DR   OMA; FRQSKPA; -.
DR   Proteomes; UP000002439; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR   GO; GO:0006413; P:translational initiation; IBA:GO_Central.
DR   Gene3D; 3.40.50.10050; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00100_A; IF_2_A; 1.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR029459; EFTU-type.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR004544; TF_aIF-2_arc.
DR   InterPro; IPR015760; TIF_IF2.
DR   InterPro; IPR023115; TIF_IF2_dom3.
DR   InterPro; IPR036925; TIF_IF2_dom3_sf.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   PANTHER; PTHR43381; PTHR43381; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF14578; GTP_EFTU_D4; 1.
DR   Pfam; PF11987; IF-2; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF52156; SSF52156; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00491; aIF-2; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Initiation factor; Nucleotide-binding; Protein biosynthesis;
KW   Reference proteome.
FT   CHAIN           1..589
FT                   /note="Probable translation initiation factor IF-2"
FT                   /id="PRO_0000137306"
FT   DOMAIN          4..225
FT                   /note="tr-type G"
FT   REGION          13..20
FT                   /note="G1"
FT                   /evidence="ECO:0000250"
FT   REGION          38..42
FT                   /note="G2"
FT                   /evidence="ECO:0000250"
FT   REGION          79..82
FT                   /note="G3"
FT                   /evidence="ECO:0000250"
FT   REGION          133..136
FT                   /note="G4"
FT                   /evidence="ECO:0000250"
FT   REGION          201..203
FT                   /note="G5"
FT                   /evidence="ECO:0000250"
FT   BINDING         13..20
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         79..83
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         133..136
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ   SEQUENCE   589 AA;  65506 MW;  2B9A24857BF3940F CRC64;
     MSQVRSPFVV VMGHVDVGKT LLLDKIRGTS VAYREPGMIT QHIGMSFVPW SAVEKFAGPL
     VDRLRLRGRI WIPGFLFIDT PGHAAFSNLR KRGGSVADLA ILVVDITSGL EEQGVESLKL
     IQSRGVPFVI AANKLDRIYG WKSVENRPFL FAVEDQEWHA VATLEERIGK LIEELSRHGI
     DADRYDRVRD FSKQVPIVPT SAVTGEGIAD LLLVLAGVSQ RFIPRERLQV REGPAKGVVM
     EVKEERGLGV VADVILYDGT LRKGDVLVTA SLEGPKEARV RMLIMPKPLE EMRDPEDKFM
     AVEEVKAAAG VRVVADGLEG VVAGSPLIAV WDPREIPNAC NLVKEEISEI RIESDKEGVI
     VRADTFGTLE SIVLFLRQQG VPVRKADVGP PTHKDVVEAV LSRRKNPIYG VILAFNVKTP
     PEVEKEAMSS GIKIIAGEIL YRIFDEYIKW SQEVRTKTIE QILSQLTRPG KIQILPGFVF
     RRSDPAIVGV KVLAGTIKPG VTLVKDGREV GRIMQIQKTG RAINEAAAGD EVAISIHGDV
     IVGRQIKEGD ILYVYVPDDQ ARQWLFQYRQ YLREDELKAL EEYLKSRRK