IF2P_PYRHO
ID IF2P_PYRHO Reviewed; 1044 AA.
AC O58822;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Probable translation initiation factor IF-2;
DE Contains:
DE RecName: Full=Pho infB intein;
DE AltName: Full=Pho IF2 intein;
GN Name=infB; OrderedLocusNames=PH1095;
OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS 100139 / OT-3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=70601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
CC -!- FUNCTION: Function in general translation initiation by promoting the
CC binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC along with eIF-2 (By similarity). {ECO:0000250}.
CC -!- PTM: This protein undergoes a protein self splicing that involves a
CC post-translational excision of the intervening region (intein) followed
CC by peptide ligation. {ECO:0000305}.
CC -!- MISCELLANEOUS: The intein interrupts the GTP-binding site.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000305}.
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DR EMBL; BA000001; BAA30194.1; -; Genomic_DNA.
DR PIR; H71049; H71049.
DR AlphaFoldDB; O58822; -.
DR SMR; O58822; -.
DR STRING; 70601.3257511; -.
DR MEROPS; N10.004; -.
DR EnsemblBacteria; BAA30194; BAA30194; BAA30194.
DR KEGG; pho:PH1095; -.
DR eggNOG; arCOG01560; Archaea.
DR eggNOG; arCOG03151; Archaea.
DR OMA; DHGKCLL; -.
DR Proteomes; UP000000752; Chromosome.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR Gene3D; 3.10.28.10; -; 1.
DR Gene3D; 3.40.50.10050; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00100_A; IF_2_A; 1.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR029459; EFTU-type.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR004544; TF_aIF-2_arc.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR43381; PTHR43381; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF14578; GTP_EFTU_D4; 1.
DR Pfam; PF11987; IF-2; 1.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF51294; SSF51294; 1.
DR SUPFAM; SSF52156; SSF52156; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF55608; SSF55608; 1.
DR TIGRFAMs; TIGR00491; aIF-2; 1.
DR TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51722; G_TR_2; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 3: Inferred from homology;
KW Autocatalytic cleavage; Endonuclease; GTP-binding; Hydrolase;
KW Initiation factor; Intron homing; Nuclease; Nucleotide-binding;
KW Protein biosynthesis; Protein splicing.
FT CHAIN 1..22
FT /note="Probable translation initiation factor IF-2, 1st
FT part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000014493"
FT CHAIN 23..466
FT /note="Pho infB intein"
FT /evidence="ECO:0000255"
FT /id="PRO_0000014494"
FT CHAIN 467..1044
FT /note="Probable translation initiation factor IF-2, 2nd
FT part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000014495"
FT DOMAIN 173..265
FT /note="DOD-type homing endonuclease"
FT DOMAIN 451..668
FT /note="tr-type G"
FT BINDING 524..528
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 578..581
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
SQ SEQUENCE 1044 AA; 118951 MW; 907C7A84D0ACB3F4 CRC64;
MSYVKRIRQP IIAVLGHVDH GKCLLPEERV ILPDYGPITL EELFNMTKET VFKDEEKEVR
KLGIRMPVAG VDGRVRLLEG PYVWKVRYKG KMLRVKLKDW HSVAVTPEHP FLTTRGWVRA
DQLKPGDYVA VPKILPGKDD KEKFLQYVHE KLKGKVHIKL PSSDEEWETF FYFAGTIFGR
ENSVNPEGLT HEVKALLELF KVLFEYPREV LRVLFMAPVR YVANFLRGFF DINGYVNGEE
LRVEVRGAPH EVLEELSLIL LRLGIVSKIY PTSLAISGRR NLELFRRYIG FSEKQKAKEL
EGIIRRSENS ESYPIFEELR RIRLLFGFTR AELSSTIPLY SKYESKEAPS YEILMKILNT
IEKGSKDLNK KITILEGRVR DHEYIEEFKR EGLIKDGKLT ELGKELLEVW RNREFDSRDV
NYLRNIIENF VFLPVEKIEE FEYDGYVYDV TTETHNFIAN GILVHNTTLL DKIRKTNVAA
KEAGGITQHI GATEVPIDVV KKIAGPLIKL WKAEIRLPGL LFIDTPGHEA FTSLRARGGS
LADLAVLVID VNEGFQPQTI ESIEILRRYR TPFVVAANKI DRIRGWVIEE DEPFLMNIKR
QDQRAIQELE TKLWELIGKF YEFGFQANRF DRVQNFTREL AIVPISAKYG IGIAELLVLI
AGLSQKYLEE KLKIEVEGPA RGTILEVREE PGLGHTIDVI IYDGTLHKDD TIVVGGKDKA
IVTKVRALLK PKPLDEIRDP RFRFDYVDEV TAAAGVKIAA PGLEEALAGS PVIAAPTPED
VERAKEEIMR QIESVVISTD KVGVIVKADT LGSLEALSKE LQEKEIPIRK ADVGNISKTD
VMEALSVKEE NPKYGVILGF NVKVNEDAKE VAKAKEVPIF VGNIIYKLIE DYEAWIKEEE
EKRKRELLAK VTFPGVIKLY PDERYVFRRS NPAIVGIEVL EGRIKPGVTL IKQNGQKVGT
IRSIKSRDEF LQEARKGQAV AIAIEGAIVG RHIHPGETLY VDLSRDDAII LLKHLRDVLE
DTDIKALKMI AQVKAKEDPF WRAV
