IF2P_THEKO
ID IF2P_THEKO Reviewed; 1144 AA.
AC Q5JGR9;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Probable translation initiation factor IF-2;
DE Contains:
DE RecName: Full=Pko infB intein;
DE AltName: Full=Pko IF2 intein;
GN Name=infB; OrderedLocusNames=TK1305;
OS Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
OS (Pyrococcus kodakaraensis (strain KOD1)).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Thermococcus.
OX NCBI_TaxID=69014;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-918 / JCM 12380 / KOD1;
RX PubMed=15710748; DOI=10.1101/gr.3003105;
RA Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.;
RT "Complete genome sequence of the hyperthermophilic archaeon Thermococcus
RT kodakaraensis KOD1 and comparison with Pyrococcus genomes.";
RL Genome Res. 15:352-363(2005).
CC -!- FUNCTION: Function in general translation initiation by promoting the
CC binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC along with eIF-2 (By similarity). {ECO:0000250}.
CC -!- PTM: This protein undergoes a protein self splicing that involves a
CC post-translational excision of the intervening region (intein) followed
CC by peptide ligation. {ECO:0000305}.
CC -!- MISCELLANEOUS: The intein interrupts the GTP-binding site.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000305}.
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DR EMBL; AP006878; BAD85494.1; -; Genomic_DNA.
DR AlphaFoldDB; Q5JGR9; -.
DR SMR; Q5JGR9; -.
DR IntAct; Q5JGR9; 1.
DR MINT; Q5JGR9; -.
DR STRING; 69014.TK1305; -.
DR PRIDE; Q5JGR9; -.
DR EnsemblBacteria; BAD85494; BAD85494; TK1305.
DR KEGG; tko:TK1305; -.
DR PATRIC; fig|69014.16.peg.1277; -.
DR eggNOG; arCOG01560; Archaea.
DR eggNOG; arCOG03151; Archaea.
DR eggNOG; arCOG03158; Archaea.
DR HOGENOM; CLU_002656_3_4_2; -.
DR InParanoid; Q5JGR9; -.
DR OMA; DHGKCLL; -.
DR PhylomeDB; Q5JGR9; -.
DR Proteomes; UP000000536; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR GO; GO:0006413; P:translational initiation; IBA:GO_Central.
DR Gene3D; 3.10.28.10; -; 1.
DR Gene3D; 3.40.50.10050; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00100_A; IF_2_A; 1.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR029459; EFTU-type.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR004544; TF_aIF-2_arc.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR43381; PTHR43381; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF14578; GTP_EFTU_D4; 1.
DR Pfam; PF11987; IF-2; 1.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF51294; SSF51294; 1.
DR SUPFAM; SSF52156; SSF52156; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF55608; SSF55608; 1.
DR TIGRFAMs; TIGR00491; aIF-2; 1.
DR TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51722; G_TR_2; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 3: Inferred from homology;
KW Autocatalytic cleavage; Endonuclease; GTP-binding; Hydrolase;
KW Initiation factor; Intron homing; Nuclease; Nucleotide-binding;
KW Protein biosynthesis; Protein splicing; Reference proteome.
FT CHAIN 1..20
FT /note="Probable translation initiation factor IF-2, 1st
FT part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000014496"
FT CHAIN 21..566
FT /note="Pko infB intein"
FT /evidence="ECO:0000255"
FT /id="PRO_0000014497"
FT CHAIN 567..1144
FT /note="Probable translation initiation factor IF-2, 2nd
FT part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000014498"
FT DOMAIN 232..362
FT /note="DOD-type homing endonuclease"
FT DOMAIN 551..768
FT /note="tr-type G"
FT BINDING 624..628
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 678..681
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
SQ SEQUENCE 1144 AA; 129904 MW; 09306CD92780023F CRC64;
MTKRIRQPII AVLGHVDHGK CLLPDEKVIL PEHGPITLKG LFDLAKETVV ADNEKEIRKL
GAKLTIVGED GRLRVLESPY VWKVRHRGKM LRVKLKNWHS VSVTPEHPFL TTRGWVRADQ
LKPGDYVAVP RVIHGNESDE RFVSFVYEKL KNDELIAKLR GEVLSKISSE FKGDRAYKVE
RNVFRWEDIE RLNLWDEVER VAFTPRMHRS GKPLHYVKLP RSPEEWEAFF YFAGVMFGDG
SQDKIANNDV EVYEELKKLS VLGVAVKRVE RTTSYEIELT NGKNALLRLL RVLFEYPERQ
KAKSIRVPRI LFIAPRKYVS RFLRGYFDAD GHVSLKDARI EVTSASQEFL EDLSLLLLRF
GIVSKIYRSD YTTLVISGRR NLDLFRRYIG FSVKNKAEAL EKAIKKSRRS ESYPIFEELK
RLRLLFGFTR TELNSNVPFY GKYESEEAPS YETLMRILDA IEKGSINLDK KIAVLEGRIR
DHNYIKAFEK DGLIKDGKLT ELGRELLEVW RNREFDSSDV DYIRNLAENL VFIPVEDIEE
FEYEGYVYDV TTETHNFVAN GILVHNTTLL DRIRHTNVAG KEAGGITQHI GATEVPIDVV
KQLAGPLIKL WKGEIKLPGL LFIDTPGHEA FTSLRARGGS LADLAVLVVD INEGFQPQTI
ESIEILRRYR TPFIVAANKI DRIKGWVIEE DEPFLVNIKK QDQRAIQELE TKLWELIGKF
YEMGFNANRF DRVQDFTREL AIVPISAKYG IGIPELLVLI AGLSQKYLEE KLKIEVEGPA
RGTILEVREE VGLGTTIDVI IYDGTLRKDD TIVVGGKDKA IVTKIRALLK PKPLDEIRDP
RFRFDQVDEV TAAAGVKIAA PGLEEALAGS PVIAARSEEE IEKAKQEILS QIQSVVINTG
KIGVIVKADT LGSLEALSKE LQEKGIPIRK ADVGNISKTD VMEALSVKEE EPKYGVVLGF
NVKVNEDAEE VANAKGVPIF VGNIIYKLIE DYEAWVKEEE EKRKRELLKN VTFPGVIRLY
PDERYVFRRS KPAIVGVEVL EGRIRPGVTL IKETGEKVGV IKSIKNKNDF VQEAKKGDAV
AIAIEGAIVG RHIHPGETLY VDLSKNDVII LAKQLKDELE ETDIKALKMT AKVKAKEDPF
WRAV
