ID   APE1_AERPE              Reviewed;         222 AA.
AC   O73942;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 2.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Homing endonuclease I-ApeI;
DE            EC=3.1.-.-;
DE   AltName: Full=rRNA intron-encoded homing endonuclease 1;
GN   Name=apeI; OrderedLocusNames=APE_1929.1;
OS   Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 /
OS   K1).
OC   Archaea; Crenarchaeota; Thermoprotei; Desulfurococcales;
OC   Desulfurococcaceae; Aeropyrum.
OX   NCBI_TaxID=272557;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1;
RX   PubMed=9658008; DOI=10.1128/jb.180.14.3635-3643.1998;
RA   Nomura N., Sako Y., Uchida A.;
RT   "Molecular characterization and postsplicing fate of three introns within
RT   the single rRNA operon of the hyperthermophilic archaeon Aeropyrum pernix
RT   K1.";
RL   J. Bacteriol. 180:3635-3643(1998).
RN   [2]
RP   SEQUENCE REVISION.
RA   Nomura N.;
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1;
RX   PubMed=10382966; DOI=10.1093/dnares/6.2.83;
RA   Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K.,
RA   Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A.,
RA   Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S.,
RA   Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A.,
RA   Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H.;
RT   "Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon,
RT   Aeropyrum pernix K1.";
RL   DNA Res. 6:83-101(1999).
RN   [4]
RP   CHARACTERIZATION.
RC   STRAIN=ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1;
RX   PubMed=16049020; DOI=10.1093/nar/gni118;
RA   Nomura N., Morinaga Y., Shirai N., Sako Y.;
RT   "I-ApeI: a novel intron-encoded LAGLIDADG homing endonuclease from the
RT   archaeon, Aeropyrum pernix K1.";
RL   Nucleic Acids Res. 33:E116-E116(2005).
CC   -!- FUNCTION: Endonuclease involved in 16S rRNA intron I-alpha homing.
CC       Recognizes the minimal target 5'-GCAAGGCTGAAACTTAAAGG-3'; generates 4
CC       base 3' protruding ends 5'-AAAC-3' and 5'-GTTT-3'.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC       Note=1-5 mM manganese gives higher cleavage activity than the same
CC       concentration of magnesium.;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 7.5.;
CC       Temperature dependence:
CC         Optimum temperature is 90 degrees Celsius. Active from 70 to 90
CC         degrees Celsius.;
CC   -!- SUBUNIT: Probably functions as a monomer.
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DR   EMBL; AB008745; BAA31987.2; -; Genomic_DNA.
DR   EMBL; BA000002; BAA80936.2; -; Genomic_DNA.
DR   PIR; C72581; C72581.
DR   AlphaFoldDB; O73942; -.
DR   SMR; O73942; -.
DR   EnsemblBacteria; BAA80936; BAA80936; APE_1929.1.
DR   KEGG; ape:APE_1929.1; -.
DR   eggNOG; arCOG03156; Archaea.
DR   OMA; HRITIWQ; -.
DR   Proteomes; UP000002518; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.28.10; -; 1.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR004860; LAGLIDADG_2.
DR   Pfam; PF14528; LAGLIDADG_3; 1.
DR   SUPFAM; SSF55608; SSF55608; 2.
PE   1: Evidence at protein level;
KW   Endonuclease; Hydrolase; Intron homing; Nuclease; Reference proteome.
FT   CHAIN           1..222
FT                   /note="Homing endonuclease I-ApeI"
FT                   /id="PRO_0000192789"
SQ   SEQUENCE   222 AA;  25031 MW;  ED9CED2EC75CC736 CRC64;
     MDGYALFYLV GALRDGSVYR YSRNYYVIWY SSNKDYLERV IVSKLRILGF HNVRVYQYKR
     GAYRVRISSK QLFHILVNQF EHPLSTSSRK TPWPTPQRVK DGPLALQIEY VKGFVDAEGS
     VIKSSKGVQV DVSQQIMEPL KFLAQVLEKV GVKVTGIYLG SDGVWRLRIA SLASLRRFAH
     YIGFRHPCKS KKLNELLGRP LPGPSKLKGI GGGAPQGVEP AA