ID   IF2_AGRFC               Reviewed;         913 AA.
AC   Q8UJ51;
DT   15-DEC-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN   Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=Atu0087;
GN   ORFNames=AGR_C_131;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- FUNCTION: One of the essential components for the initiation of protein
CC       synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis
CC       and promotes its binding to the 30S ribosomal subunits. Also involved
CC       in the hydrolysis of GTP during the formation of the 70S ribosomal
CC       complex. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00100}.
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DR   EMBL; AE007869; AAK85907.1; -; Genomic_DNA.
DR   PIR; AB2587; AB2587.
DR   PIR; B97369; B97369.
DR   RefSeq; NP_353122.1; NC_003062.2.
DR   RefSeq; WP_010970645.1; NC_003062.2.
DR   AlphaFoldDB; Q8UJ51; -.
DR   SMR; Q8UJ51; -.
DR   STRING; 176299.Atu0087; -.
DR   EnsemblBacteria; AAK85907; AAK85907; Atu0087.
DR   KEGG; atu:Atu0087; -.
DR   PATRIC; fig|176299.10.peg.80; -.
DR   eggNOG; COG0532; Bacteria.
DR   eggNOG; COG3064; Bacteria.
DR   HOGENOM; CLU_006301_10_0_5; -.
DR   OMA; NRDNRTG; -.
DR   PhylomeDB; Q8UJ51; -.
DR   BioCyc; AGRO:ATU0087-MON; -.
DR   Proteomes; UP000000813; Chromosome circular.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   CDD; cd03702; IF2_mtIF2_II; 1.
DR   Gene3D; 3.40.50.10050; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00100_B; IF_2_B; 1.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR013575; IF2_assoc_dom_bac.
DR   InterPro; IPR044145; IF2_II.
DR   InterPro; IPR006847; IF2_N.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR000178; TF_IF2_bacterial-like.
DR   InterPro; IPR015760; TIF_IF2.
DR   InterPro; IPR023115; TIF_IF2_dom3.
DR   InterPro; IPR036925; TIF_IF2_dom3_sf.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   PANTHER; PTHR43381; PTHR43381; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF11987; IF-2; 1.
DR   Pfam; PF08364; IF2_assoc; 1.
DR   Pfam; PF04760; IF2_N; 1.
DR   SUPFAM; SSF50447; SSF50447; 2.
DR   SUPFAM; SSF52156; SSF52156; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00487; IF-2; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
DR   PROSITE; PS01176; IF2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; GTP-binding; Initiation factor; Nucleotide-binding;
KW   Protein biosynthesis; Reference proteome.
FT   CHAIN           1..913
FT                   /note="Translation initiation factor IF-2"
FT                   /id="PRO_0000137164"
FT   DOMAIN          411..578
FT                   /note="tr-type G"
FT   REGION          1..322
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          420..427
FT                   /note="G1"
FT                   /evidence="ECO:0000250"
FT   REGION          445..449
FT                   /note="G2"
FT                   /evidence="ECO:0000250"
FT   REGION          466..469
FT                   /note="G3"
FT                   /evidence="ECO:0000250"
FT   REGION          520..523
FT                   /note="G4"
FT                   /evidence="ECO:0000250"
FT   REGION          556..558
FT                   /note="G5"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        42..59
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        65..79
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        86..115
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        128..177
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        240..256
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        276..292
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        303..322
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         420..427
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         466..470
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         520..523
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ   SEQUENCE   913 AA;  98822 MW;  2BF1CEE264C484D8 CRC64;
     MTDNNDDKTL NAPAKKTLTL KPGGMNQGTV RQDMGRGRTN AVVVETRKRR PHRPEDEKPV
     QPVVAAPKPA APAPVAARPQ APQPRIHQPG GQQQRPGSSQ SQQRSGSSAP QQRQADRPRG
     NVLHDLSAGE MEARRRALME AQARDVVEAK QRAEDEARRK VEEEQRIAAE KMEAANRAAE
     EAAAAKVAAS QPAAEVRAEP ASERPAAAAA PAPRTDARPQ SAAAAPRSAP ATPDAAAPRG
     RRTGGDDEDD RGAVRRGSSL PARGKVVAPA PAKPAARLKT EEERRRGKLT VTSNLEEDGT
     PRGRSMASMR RRQEKFRRSQ MQETREKVLR EVILPETITI QELSQRMSER AVDVIKFLMK
     EGQMLKPGDV IDADLAELIA VEFGHTVKRV SESDVEEGIF NQTDDEGEMV SRPPVVTIMG
     HVDHGKTSLL DAIRQANVVS GEAGGITQHI GAYQVEKNGH KITFIDTPGH AAFTAMRARG
     AQATDIAVLV VAADDSVMPQ TIESINHAKA AGVPIVVAIN KIDKHEANPD KVRQQLLQHE
     VFVESMGGEV LDVEVSAKNK LNLDKLLEAI LLQAEILDLK ADPSRTAEGT VIEAELDRGR
     GAVATVLVQK GTLKPGQIIV AGDQWGRVRA LVNDKGDHVK EAGPAMPVEI LGLSGTPSAG
     DRFAVVENES RAREISEYRQ RLARDKAVAR QTGQRGSLEQ MMSQLQTSGL KEFPLVIKAD
     VQGSVEAIIA SLDKLGTDEV RARVVHSGAG AITESDISLA EASNAAIIGF NVRANAQART
     ASERAGIEIR YYNIIYDLVD DVKAAMSGLL SPERRETFLG NAEILEVFNI TKVGKVAGCR
     VVEGKVERGA GVRLVRDNVV IHEGKLKTLK RFKDEVNEVP VGQECGMAFE NYEDIRAGDT
     IECFRVEHIT RTL