4CL2_TOBAC
ID 4CL2_TOBAC Reviewed; 542 AA.
AC O24146;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=4-coumarate--CoA ligase 2;
DE Short=4CL 2;
DE EC=6.2.1.12;
DE AltName: Full=4-coumaroyl-CoA synthase 2;
GN Name=4CL2;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8819324; DOI=10.1104/pp.112.1.193;
RA Lee D., Douglas C.J.;
RT "Two divergent members of a tobacco 4-coumarate:coenzyme A ligase (4CL)
RT gene family. cDNA structure, gene inheritance and expression, and
RT properties of recombinant proteins.";
RL Plant Physiol. 112:193-205(1996).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-4-coumarate + ATP + CoA = (E)-4-coumaroyl-CoA + AMP +
CC diphosphate; Xref=Rhea:RHEA:19641, ChEBI:CHEBI:12876,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:85008, ChEBI:CHEBI:456215; EC=6.2.1.12;
CC -!- PATHWAY: Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene
CC biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step
CC 1/2.
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000305}.
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DR EMBL; U50846; AAB18638.1; -; mRNA.
DR PIR; T03789; T03789.
DR RefSeq; NP_001312554.1; NM_001325625.1.
DR PDB; 5BSM; X-ray; 2.32 A; A/B=1-542.
DR PDB; 5BSR; X-ray; 1.50 A; A=1-542.
DR PDB; 5BST; X-ray; 1.61 A; A=1-542.
DR PDB; 5BSU; X-ray; 1.75 A; A=1-542.
DR PDB; 5BSV; X-ray; 1.70 A; A=1-542.
DR PDB; 5BSW; X-ray; 2.10 A; A/B=1-542.
DR PDB; 5U95; X-ray; 2.25 A; A/B/C/D=1-542.
DR PDBsum; 5BSM; -.
DR PDBsum; 5BSR; -.
DR PDBsum; 5BST; -.
DR PDBsum; 5BSU; -.
DR PDBsum; 5BSV; -.
DR PDBsum; 5BSW; -.
DR PDBsum; 5U95; -.
DR AlphaFoldDB; O24146; -.
DR SMR; O24146; -.
DR STRING; 4097.O24146; -.
DR GeneID; 107797004; -.
DR KEGG; nta:107797004; -.
DR BRENDA; 6.2.1.12; 3645.
DR UniPathway; UPA00372; UER00547.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0016207; F:4-coumarate-CoA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016405; F:CoA-ligase activity; IBA:GO_Central.
DR GO; GO:0009698; P:phenylpropanoid metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.50.12780; -; 1.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR042099; ANL_N_sf.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF13193; AMP-binding_C; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Ligase; Nucleotide-binding;
KW Phenylpropanoid metabolism; Reference proteome.
FT CHAIN 1..542
FT /note="4-coumarate--CoA ligase 2"
FT /id="PRO_0000193041"
FT HELIX 27..31
FT /evidence="ECO:0007829|PDB:5BSR"
FT TURN 32..34
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 35..37
FT /evidence="ECO:0007829|PDB:5BSR"
FT TURN 38..40
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 41..46
FT /evidence="ECO:0007829|PDB:5BSR"
FT TURN 47..49
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 52..54
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 55..71
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 79..82
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 88..99
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 103..106
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 113..123
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 126..130
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 132..137
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 139..144
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 148..153
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 163..166
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 170..172
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 183..185
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 193..195
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 197..202
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 203..214
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 216..218
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 228..231
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 238..243
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 245..251
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 254..257
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 263..273
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 277..280
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 282..289
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 292..296
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 304..307
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 314..323
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 328..331
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 333..335
FT /evidence="ECO:0007829|PDB:5BSW"
FT HELIX 336..338
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 339..344
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 346..348
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 349..351
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 361..363
FT /evidence="ECO:0007829|PDB:5BSU"
FT STRAND 368..372
FT /evidence="ECO:0007829|PDB:5BSR"
FT TURN 374..376
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 386..392
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 397..399
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 403..409
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 416..424
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 430..435
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 436..438
FT /evidence="ECO:0007829|PDB:5BSW"
FT STRAND 440..442
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 445..447
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 449..457
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 462..472
FT /evidence="ECO:0007829|PDB:5BSR"
FT TURN 473..475
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 476..485
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 493..501
FT /evidence="ECO:0007829|PDB:5BSR"
FT HELIX 506..508
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 512..515
FT /evidence="ECO:0007829|PDB:5BSR"
FT STRAND 523..525
FT /evidence="ECO:0007829|PDB:5U95"
FT HELIX 529..535
FT /evidence="ECO:0007829|PDB:5BSR"
SQ SEQUENCE 542 AA; 59480 MW; CB5579AEDFCFC003 CRC64;
MEKDTKQVDI IFRSKLPDIY IPNHLPLHSY CFENISEFSS RPCLINGANK QIYTYADVEL
NSRKVAAGLH KQGIQPKDTI MILLPNSPEF VFAFIGASYL GAISTMANPL FTPAEVVKQA
KASSAKIIVT QACHVNKVKD YAFENDVKII CIDSAPEGCL HFSVLTQANE HDIPEVEIQP
DDVVALPYSS GTTGLPKGVM LTHKGLVTSV AQQVDGENPN LYIHSEDVML CVLPLFHIYS
LNSVLLCGLR VGAAILIMQK FDIVSFLELI QRYKVTIGPF VPPIVLAIAK SPMVDDYDLS
SVRTVMSGAA PLGKELEDTV RAKFPNAKLG QGYGMTEAGP VLAMCLAFAK EPFEIKSGAC
GTVVRNAEMK IVDPKTGNSL PRNQSGEICI RGDQIMKGYL NDPEATARTI DKEGWLYTGD
IGYIDDDDEL FIVDRLKELI KYKGFQVAPA ELEALLLNHP NISDAAVVPM KDEQAGEVPV
AFVVRSNGST ITEDEVKDFI SKQVIFYKRI KRVFFVDAIP KSPSGKILRK DLRAKLAAGL
PN
