IF2_BLOFL
ID IF2_BLOFL Reviewed; 897 AA.
AC Q7VQM3;
DT 15-DEC-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=Bfl104;
OS Blochmannia floridanus.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; ant endosymbionts; Candidatus Blochmannia.
OX NCBI_TaxID=203907;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12886019; DOI=10.1073/pnas.1533499100;
RA Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F.,
RA Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B.,
RA van Ham R.C.H.J., Gross R., Moya A.;
RT "The genome sequence of Blochmannia floridanus: comparative analysis of
RT reduced genomes.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003).
CC -!- FUNCTION: One of the essential components for the initiation of protein
CC synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis
CC and promotes its binding to the 30S ribosomal subunits. Also involved
CC in the hydrolysis of GTP during the formation of the 70S ribosomal
CC complex. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00100}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00100}.
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DR EMBL; BX248583; CAD83625.1; -; Genomic_DNA.
DR AlphaFoldDB; Q7VQM3; -.
DR SMR; Q7VQM3; -.
DR STRING; 203907.Bfl104; -.
DR PRIDE; Q7VQM3; -.
DR EnsemblBacteria; CAD83625; CAD83625; Bfl104.
DR KEGG; bfl:Bfl104; -.
DR eggNOG; COG0532; Bacteria.
DR HOGENOM; CLU_006301_6_3_6; -.
DR OMA; NRDNRTG; -.
DR Proteomes; UP000002192; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR CDD; cd03702; IF2_mtIF2_II; 1.
DR Gene3D; 3.40.50.10050; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00100_B; IF_2_B; 1.
DR InterPro; IPR009061; DNA-bd_dom_put_sf.
DR InterPro; IPR013575; IF2_assoc_dom_bac.
DR InterPro; IPR044145; IF2_II.
DR InterPro; IPR006847; IF2_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR000178; TF_IF2_bacterial-like.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR43381; PTHR43381; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF11987; IF-2; 1.
DR Pfam; PF08364; IF2_assoc; 1.
DR Pfam; PF04760; IF2_N; 1.
DR SUPFAM; SSF46955; SSF46955; 1.
DR SUPFAM; SSF50447; SSF50447; 2.
DR SUPFAM; SSF52156; SSF52156; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00487; IF-2; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51722; G_TR_2; 1.
DR PROSITE; PS01176; IF2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; GTP-binding; Initiation factor; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome.
FT CHAIN 1..897
FT /note="Translation initiation factor IF-2"
FT /id="PRO_0000137185"
FT DOMAIN 402..570
FT /note="tr-type G"
FT REGION 411..418
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 436..440
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 458..461
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 512..515
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 548..550
FT /note="G5"
FT /evidence="ECO:0000250"
FT BINDING 411..418
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT BINDING 458..462
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT BINDING 512..515
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ SEQUENCE 897 AA; 100775 MW; D2DAFEF1557A7D15 CRC64;
MTIQSFAIEV QMSVDYLIQQ FLDIGITKTK FDFITQSEKE ILFRHMNVNK IAVFNKLFLQ
RKTHSTLNVS STNGKSKKVK VEVRKKRVYM VPCSIRKSHV SNNTNILLVE NDNSKDKYKS
DDCNELSELV ACDENKISSN ENQVMCFISQ KEKLDHFTIR QDYVRTNELV KDVNQDLDHQ
IRDRKGLSNS VCCDVLLNVQ DEQHDDNNFS CGLSDDMAVS NNIPEHDKQK LENVRKNLTD
RLRSTRTRNL SKLVKQNKHN NSKVCMIYES DKDEKLYVLP SSRVYKNKRK QSILVQSFNK
PMQKTVRDVV IGETISVAEL SNKMSIKSSS MIKMMMKLGL MVTINQNLDQ ETAQLVVEEM
GHNAILRREN ALEELIMHDN NNDYQNISSK LDNAKNDMGY KNRAPIVTIM GHVDHGKTSL
LDYIRSTNVA SSESGGITQN IGAYCVQLAN NDMVTFIDTP GHEAFTDMRA RGIQLTDIVV
LVVAADDGVM PQTVEAIQYI RDGNLPVIIA INKIDKSVTN VERIKNELNS YGFIPEEWGG
HTQFVNVSAV SGEGVNDLLD SILVQSEILE LKSMHHGLAK AVVIESSLDR SRGPVVTVLV
RSGELKCGDI VLCGTEYGRV RAMRDSLGFD VIKAGPSIPV ELLGLSGIPG TGEWLVVVNN
EKKAREVALY RKEKKREIKL ARKSEKTIIE NFNSMDIIKI KELNFIIKSD TQGSMEVICE
SLKKLSTNKM VMKILSASIG NVTETDAVLA LSSHSRILAF NVKVDLSAKN IIELNHINIQ
YYSVIYSLLD EVKELISSTI APQVDFKVIG IAKVHNIFQS PRYGTIAGCM VTQGVIKLHK
QIKIVRNGII MYKGVLDSLR HFKNDVSEVK IGVECGIGIK NYSDVCSGDI IEVLDKI
