IF2_BRUA4
ID IF2_BRUA4 Reviewed; 964 AA.
AC A6WWW5;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=Oant_0747;
OS Brucella anthropi (strain ATCC 49188 / DSM 6882 / CCUG 24695 / JCM 21032 /
OS LMG 3331 / NBRC 15819 / NCTC 12168 / Alc 37) (Ochrobactrum anthropi).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=439375;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49188 / DSM 6882 / CCUG 24695 / JCM 21032 / LMG 3331 / NBRC
RC 15819 / NCTC 12168 / Alc 37;
RX PubMed=21685287; DOI=10.1128/jb.05335-11;
RA Chain P.S., Lang D.M., Comerci D.J., Malfatti S.A., Vergez L.M., Shin M.,
RA Ugalde R.A., Garcia E., Tolmasky M.E.;
RT "Genome of Ochrobactrum anthropi ATCC 49188 T, a versatile opportunistic
RT pathogen and symbiont of several eukaryotic hosts.";
RL J. Bacteriol. 193:4274-4275(2011).
CC -!- FUNCTION: One of the essential components for the initiation of protein
CC synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis
CC and promotes its binding to the 30S ribosomal subunits. Also involved
CC in the hydrolysis of GTP during the formation of the 70S ribosomal
CC complex. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00100}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00100}.
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DR EMBL; CP000758; ABS13469.1; -; Genomic_DNA.
DR RefSeq; WP_012090991.1; NC_009667.1.
DR AlphaFoldDB; A6WWW5; -.
DR SMR; A6WWW5; -.
DR STRING; 439375.Oant_0747; -.
DR PRIDE; A6WWW5; -.
DR EnsemblBacteria; ABS13469; ABS13469; Oant_0747.
DR KEGG; oan:Oant_0747; -.
DR PATRIC; fig|439375.7.peg.789; -.
DR eggNOG; COG0532; Bacteria.
DR eggNOG; COG3064; Bacteria.
DR HOGENOM; CLU_006301_10_0_5; -.
DR OMA; QVRPEMI; -.
DR OrthoDB; 347113at2; -.
DR PhylomeDB; A6WWW5; -.
DR Proteomes; UP000002301; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR CDD; cd03702; IF2_mtIF2_II; 1.
DR Gene3D; 3.40.50.10050; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00100_B; IF_2_B; 1.
DR InterPro; IPR013575; IF2_assoc_dom_bac.
DR InterPro; IPR044145; IF2_II.
DR InterPro; IPR006847; IF2_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR000178; TF_IF2_bacterial-like.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR43381; PTHR43381; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF11987; IF-2; 1.
DR Pfam; PF08364; IF2_assoc; 1.
DR Pfam; PF04760; IF2_N; 1.
DR SUPFAM; SSF50447; SSF50447; 2.
DR SUPFAM; SSF52156; SSF52156; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00487; IF-2; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51722; G_TR_2; 1.
DR PROSITE; PS01176; IF2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; GTP-binding; Initiation factor; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome.
FT CHAIN 1..964
FT /note="Translation initiation factor IF-2"
FT /id="PRO_1000008291"
FT DOMAIN 462..629
FT /note="tr-type G"
FT REGION 1..379
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 471..478
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 496..500
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 517..520
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 571..574
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 607..609
FT /note="G5"
FT /evidence="ECO:0000250"
FT COMPBIAS 12..37
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 42..63
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 101..115
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 123..153
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 156..245
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 251..265
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 327..379
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 471..478
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT BINDING 517..521
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT BINDING 571..574
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ SEQUENCE 964 AA; 104399 MW; 9EDC08D809C53DC9 CRC64;
MSDKTNDDKT LSVNTKKTLT MKRPGVEQST VRQNFSHGRT KAVVVETKKR KFSRPDEKPE
VEAAAPKPAA PAPAAPAPAA STPAPAQAAQ PAQAAPVVRA PAPATPAPKP AAPAAPVTKP
HVAQQRPAQQ RPGGQQAQRP RPSDRSGMVL NTLSRSEMDA RRRALEEAQV REVEERARAV
EEAKRRAEED ARRAKEREES ARRQAEEEAR LKAEADARRK AEEEAAKRMP QPEARTERRD
DARPAPQGNR PQQAGRPQGN RPPQGGRPQQ GGPRPAAPSL ADAAPIPGKP LPQSQLRKTV
ASDDDDRRGG GLTAARRGAP AKPEVRAPKV VKTEDDRRRG KLTISSNLED EGRSRSLSAM
RRRQEKFKRS QMQETREKIS REVTIPETIT LQELAQRMTE RSVDIIKYLM KQGQMMKPGD
VIDADMAQLI AEEFGHTVKR VAESDVEEGI FGVADNEAAL VSRPPVVTIM GHVDHGKTSL
LDAIRHANVV SGEAGGITQH IGAYQVEQNG QKITFIDTPG HAAFTAMRAR GAQATDIAIL
VVAADDSVMP QTIESINHAK AAGVPIIVAI NKIDKPAADP QKVRTALLQH DVFVESMGGE
VLDVEVSAKN KLNLDKLLEA ILLQAEILDL KADPSRTAEG VVVEAQLDRG RGSVATVLVQ
TGTLHPGDIL VAGSEWGRVR ALVNDRGEHV KEAGPAMPVE VLGLQGTPQA GDRFAVVANE
AKAREIAEYR QRLARDKAVA RQSGARGSLE QMMNQLQVSG TKEFPLVIKG DVQGSIEAIT
NALDKLGTDE VRARIVHSGA GGITESDVSL AEASNAAIIG FNVRANKQAR DAADQQGIEI
RYYNIIYDLI DDVKAAMSGL LSPERRETFL GNAEILEVFN ITKVGKVAGC RVTEGKVERG
AGVRLIRDNV VIHEGKLKTL KRFKDEVSEV PAGQECGMAF ENYDDIRAGD VIEAFRVEHV
SRTL
