IF2_CHLPN
ID IF2_CHLPN Reviewed; 890 AA.
AC Q9Z8M1; Q9JRX1;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2001, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Translation initiation factor IF-2;
GN Name=infB; OrderedLocusNames=CPn_0317, CP_0440, CpB0327;
OS Chlamydia pneumoniae (Chlamydophila pneumoniae).
OC Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC Chlamydia/Chlamydophila group; Chlamydia.
OX NCBI_TaxID=83558;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CWL029;
RX PubMed=10192388; DOI=10.1038/7716;
RA Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W.,
RA Olinger L., Grimwood J., Davis R.W., Stephens R.S.;
RT "Comparative genomes of Chlamydia pneumoniae and C. trachomatis.";
RL Nat. Genet. 21:385-389(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AR39;
RX PubMed=10684935; DOI=10.1093/nar/28.6.1397;
RA Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O.,
RA Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S.,
RA Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J.,
RA Gwinn M.L., Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G.,
RA Salzberg S.L., Eisen J.A., Fraser C.M.;
RT "Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae
RT AR39.";
RL Nucleic Acids Res. 28:1397-1406(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=J138;
RX PubMed=10871362; DOI=10.1093/nar/28.12.2311;
RA Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K.,
RA Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T.;
RT "Comparison of whole genome sequences of Chlamydia pneumoniae J138 from
RT Japan and CWL029 from USA.";
RL Nucleic Acids Res. 28:2311-2314(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TW-183;
RA Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P.,
RA Schneider S., Pohl T., Essig A., Marre R., Melchers K.;
RT "The genome sequence of Chlamydia pneumoniae TW183 and comparison with
RT other Chlamydia strains based on whole genome sequence analysis.";
RL Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: One of the essential components for the initiation of protein
CC synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis
CC and promotes its binding to the 30S ribosomal subunits. Also involved
CC in the hydrolysis of GTP during the formation of the 70S ribosomal
CC complex (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAP98260.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE001363; AAD18466.1; -; Genomic_DNA.
DR EMBL; AE002161; AAF38279.1; -; Genomic_DNA.
DR EMBL; BA000008; BAA98527.1; -; Genomic_DNA.
DR EMBL; AE009440; AAP98260.1; ALT_INIT; Genomic_DNA.
DR PIR; E81576; E81576.
DR PIR; E86530; E86530.
DR PIR; F72093; F72093.
DR RefSeq; NP_224522.1; NC_000922.1.
DR RefSeq; WP_010892069.1; NZ_LN846995.1.
DR AlphaFoldDB; Q9Z8M1; -.
DR SMR; Q9Z8M1; -.
DR STRING; 115711.CP_0440; -.
DR EnsemblBacteria; AAD18466; AAD18466; CPn_0317.
DR EnsemblBacteria; AAF38279; AAF38279; CP_0440.
DR KEGG; cpa:CP_0440; -.
DR KEGG; cpj:infB; -.
DR KEGG; cpn:CPn_0317; -.
DR KEGG; cpt:CpB0327; -.
DR PATRIC; fig|115713.3.peg.351; -.
DR eggNOG; COG0532; Bacteria.
DR HOGENOM; CLU_006301_3_0_0; -.
DR Proteomes; UP000000583; Chromosome.
DR Proteomes; UP000000801; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR CDD; cd03702; IF2_mtIF2_II; 1.
DR Gene3D; 3.40.50.10050; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00100_B; IF_2_B; 1.
DR InterPro; IPR044145; IF2_II.
DR InterPro; IPR006847; IF2_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR000178; TF_IF2_bacterial-like.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR43381; PTHR43381; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF11987; IF-2; 1.
DR Pfam; PF04760; IF2_N; 1.
DR SUPFAM; SSF50447; SSF50447; 2.
DR SUPFAM; SSF52156; SSF52156; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00487; IF-2; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51722; G_TR_2; 1.
DR PROSITE; PS01176; IF2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; GTP-binding; Initiation factor; Nucleotide-binding;
KW Protein biosynthesis.
FT CHAIN 1..890
FT /note="Translation initiation factor IF-2"
FT /id="PRO_0000137189"
FT DOMAIN 395..564
FT /note="tr-type G"
FT REGION 31..164
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 189..266
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 404..411
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 429..433
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 450..453
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 504..507
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 540..542
FT /note="G5"
FT /evidence="ECO:0000250"
FT COMPBIAS 58..75
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 206..237
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 242..266
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 404..411
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 450..454
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 504..507
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT VARIANT 321
FT /note="T -> A (in strain: CWL029 and TW-183)"
SQ SEQUENCE 890 AA; 97105 MW; BBB4C2C269268781 CRC64;
MEKVKLTKNL KLKIKNAQLT KAAGLDKLKQ KLAQAGSSEA KSSSEKPSAK EKSVKVALAA
TSTPTASAEQ ASPESTSRRI RAKNRSSFSS SEEESSAHIP VDTSEPAPVS IADPEPELEV
VDEVCDESPE VHPVAEVLPE QPVLPETPPQ EKELEPKPVK PAEPKSVVMI KSKFGPTGKH
INHLLAKTFK APAKEEKVVA GSKSTKPVAS DKTGKPGTSE GGEQNNREKQ FNPANRSPAS
GPKRDAGKKN LTDFRDRSKK SDESLKAFTG RDRYGLNEGG EEDRWRKKRV YKPKKHYDEA
SIQRPTHIKI SLPITVKDLA TEMKLKASEV IQKLFIHGMT YVVNDILDSE TAVQFIGLEF
GCTIDIDYSE QDKLCLSNDT VRDEIQSTDP SKLVIRSPIV AFMGHVDHGK TTLIDSLRKS
NVAATEAGAI TQHMGAFCCS TPVGDITILD TPGHEAFSAM RARGAEVCDI VVLVVAGDEG
IKEQTLEAIE HAKAADIAIV VAINKCDKPN FNSETIYRQL SEINLLPEAW GGSTVTVNTS
AKTGEGLSEL LEMLALQAEV LELKADPSAR ARGLVIESEL HKGLGPVATV LIQNGSLKLG
EALVFNDCYG KVKTMHNEHN ELMKEAGPSI PVLITGLSDI PKAGDPFFVV KNEKTARDII
EARSAGQQRF ALQQKKRPNF DSMLQNKKTL KLMIKADVQG SIEALVSSIS KIKSEKVDVE
ILTNSVGEIS ESDIRLAAAS KAVLIGFHTG IESHAEPLIK SLGVRVELFT VIYHAIDAIK
EIMTSLLDPI AEEKDEGSAE IKEIFRSSQV GSIYGCIVTE GIMTRNHKVR VLRNKEILWK
GTLSSLKRVK EDVKEVRKGL ECGILLEGYQ QAQIGDVLQC YEVIYHPQKL
