ID   APEA_THENE              Reviewed;         452 AA.
AC   O86957;
DT   24-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Probable M18 family aminopeptidase 1;
DE            EC=3.4.11.-;
GN   Name=apeA;
OS   Thermotoga neapolitana.
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX   NCBI_TaxID=2337;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Z2706-MC24;
RA   Zverlov V.V., Mashchenko O.V., Liebl W., Velikodvorskaya G.A.;
RL   Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the peptidase M18 family. {ECO:0000305}.
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DR   EMBL; AJ009831; CAA08865.1; -; Genomic_DNA.
DR   AlphaFoldDB; O86957; -.
DR   SMR; O86957; -.
DR   MEROPS; M18.004; -.
DR   GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.30.250.10; -; 1.
DR   HAMAP; MF_00466; Aminopeptidase_M18_1; 1.
DR   InterPro; IPR022983; M18_aminopeptidase_1.
DR   InterPro; IPR001948; Peptidase_M18.
DR   InterPro; IPR023358; Peptidase_M18_dom2.
DR   PANTHER; PTHR28570; PTHR28570; 1.
DR   Pfam; PF02127; Peptidase_M18; 1.
DR   PRINTS; PR00932; AMINO1PTASE.
PE   3: Inferred from homology;
KW   Aminopeptidase; Hydrolase; Metal-binding; Metalloprotease; Protease; Zinc.
FT   CHAIN           1..452
FT                   /note="Probable M18 family aminopeptidase 1"
FT                   /id="PRO_0000173459"
FT   BINDING         93
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255"
FT   BINDING         168
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255"
FT   BINDING         427
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   452 AA;  50907 MW;  970EB7DC0F79F051 CRC64;
     MKMERKNVWQ HRNREEIESF SKEYMDFMGR AKTERLAVRE IRKFLEKEGF VPIEDFAGDP
     MDMAVYAVNR GKAIAAFRVV DDLKKGLNLV VAHIDSPRLD FKPNPLVEDE QIALFKTHYY
     GGIKKYHWFN IPLEIHGVLF RGDGEEIEIH VGDKPEDPVF TIPDLLPHLD KEDAKISEKF
     KGENLMLIAG TIPLSGEEKE AVKMNVLKIL NEMYGISEED FVSGEIEVVP AFPPREVGID
     RSLIGAYGQD DRICAYTALR AFLEANPKKS IGVVFLDKEE IGSDGNTGAK ARFYLRVLRQ
     ILKIQGGPEI QNSHSMRYWK KSAVISGDVC AAVNPPYKDV HDLHNAPRSG YGVALVKYTG
     ARGKYSTNDA HAEFVARVRQ VLNERNVIWQ VATLGKVDQG GGGTYAKFFA ERGADVYDMG
     PALLGMHSPF EISSKADLFE TYRAYRALLE DL