ID   4CL3_DICDI              Reviewed;         551 AA.
AC   Q54P79;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 2.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Probable 4-coumarate--CoA ligase 3;
DE            Short=4CL 3;
DE            EC=6.2.1.12;
DE   AltName: Full=4-coumaroyl-CoA synthase 3;
GN   Name=4cl3; ORFNames=DDB_G0284743;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(E)-4-coumarate + ATP + CoA = (E)-4-coumaroyl-CoA + AMP +
CC         diphosphate; Xref=Rhea:RHEA:19641, ChEBI:CHEBI:12876,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:85008, ChEBI:CHEBI:456215; EC=6.2.1.12;
CC   -!- PATHWAY: Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene
CC       biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step
CC       1/2.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000071; EAL65024.2; -; Genomic_DNA.
DR   RefSeq; XP_638379.2; XM_633287.2.
DR   AlphaFoldDB; Q54P79; -.
DR   SMR; Q54P79; -.
DR   STRING; 44689.DDB0266358; -.
DR   PaxDb; Q54P79; -.
DR   EnsemblProtists; EAL65024; EAL65024; DDB_G0284743.
DR   GeneID; 8624748; -.
DR   KEGG; ddi:DDB_G0284743; -.
DR   dictyBase; DDB_G0284743; 4cl3.
DR   eggNOG; KOG1176; Eukaryota.
DR   HOGENOM; CLU_000022_59_2_1; -.
DR   InParanoid; Q54P79; -.
DR   OMA; AVWSGDT; -.
DR   PhylomeDB; Q54P79; -.
DR   UniPathway; UPA00372; UER00547.
DR   PRO; PR:Q54P79; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0016207; F:4-coumarate-CoA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016405; F:CoA-ligase activity; IBA:GO_Central.
DR   GO; GO:0009698; P:phenylpropanoid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.300.30; -; 1.
DR   InterPro; IPR025110; AMP-bd_C.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF13193; AMP-binding_C; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; Phenylpropanoid metabolism;
KW   Reference proteome.
FT   CHAIN           1..551
FT                   /note="Probable 4-coumarate--CoA ligase 3"
FT                   /id="PRO_0000327703"
SQ   SEQUENCE   551 AA;  61163 MW;  29FD8808DCB9615B CRC64;
     MIRIINGQTY FTSKYPNIII PEKPIPHLIL KHIRYKPDQV LLVDGLTFKE YSSHFVADTI
     EKVACGLNKL NIKKGDVLGV ILPNLPEYVP IFHGTLLMGG ITSLVNPDYT IEELSHTLAT
     VSPRYLAVTL AVYEKIKNDL KRVFPSVEKV ILVDIAGQTL KEIDQLTLSS DGIVMSFNQL
     TNNNGKDYPI VRIDLTKDTA IIPFSSGTTG LFKGVCLSHY NLVSNTYQTQ TIETSTYKKN
     DSVIGVLPFF HSFGLMLHIM LMVKQGYRIV TLPKFEPVRF LELIKKYKVA MSFIVPPIAI
     MFAKSPIVDK FDLSSLRTLF CGAAPLGSEI EDLIKERFKG RLVIKQGYGA TELSPCCFVT
     PNGLVKSGSS GTLLPNLLAK IISSETGENL GMGEKGEICI KGPNVMLGYY NNEKATNEVI
     DKDGFLKTGD IGYVDEDGYF FIIDRSKELI KCKGFQVPPA ELEALLLSHP KVADACVVGL
     SKGDMGEVPR GFVVIKQNES LTEKELLDWA HPKIANYKHF RGGIFFIPAI PKSATGKLLR
     KNLKDFNNLK L