IF2_EHRRW
ID IF2_EHRRW Reviewed; 856 AA.
AC Q5HB61; Q5FCA7;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN Name=infB {ECO:0000255|HAMAP-Rule:MF_00100};
GN OrderedLocusNames=Erum4690, ERWE_CDS_04910;
OS Ehrlichia ruminantium (strain Welgevonden).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC Anaplasmataceae; Ehrlichia.
OX NCBI_TaxID=254945;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Welgevonden;
RX PubMed=15637156; DOI=10.1073/pnas.0406633102;
RA Collins N.E., Liebenberg J., de Villiers E.P., Brayton K.A., Louw E.,
RA Pretorius A., Faber F.E., van Heerden H., Josemans A., van Kleef M.,
RA Steyn H.C., van Strijp M.F., Zweygarth E., Jongejan F., Maillard J.C.,
RA Berthier D., Botha M., Joubert F., Corton C.H., Thomson N.R., Allsopp M.T.,
RA Allsopp B.A.;
RT "The genome of the heartwater agent Ehrlichia ruminantium contains multiple
RT tandem repeats of actively variable copy number.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:838-843(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Welgevonden;
RX PubMed=16547041; DOI=10.1128/jb.188.7.2533-2542.2006;
RA Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y.,
RA Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D.;
RT "Comparative genomic analysis of three strains of Ehrlichia ruminantium
RT reveals an active process of genome size plasticity.";
RL J. Bacteriol. 188:2533-2542(2006).
CC -!- FUNCTION: One of the essential components for the initiation of protein
CC synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis
CC and promotes its binding to the 30S ribosomal subunits. Also involved
CC in the hydrolysis of GTP during the formation of the 70S ribosomal
CC complex. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00100}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00100}.
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DR EMBL; CR767821; CAH58197.1; -; Genomic_DNA.
DR EMBL; CR925678; CAI26985.1; -; Genomic_DNA.
DR RefSeq; WP_011155150.1; NC_006832.1.
DR AlphaFoldDB; Q5HB61; -.
DR SMR; Q5HB61; -.
DR STRING; 254945.Erum4690; -.
DR EnsemblBacteria; CAI26985; CAI26985; ERWE_CDS_04910.
DR KEGG; eru:Erum4690; -.
DR KEGG; erw:ERWE_CDS_04910; -.
DR eggNOG; COG0532; Bacteria.
DR HOGENOM; CLU_006301_10_2_5; -.
DR OMA; VIFAMNK; -.
DR BioCyc; ERUM254945:ERUM_RS02570-MON; -.
DR Proteomes; UP000001021; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR CDD; cd03702; IF2_mtIF2_II; 1.
DR Gene3D; 3.40.50.10050; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00100_B; IF_2_B; 1.
DR InterPro; IPR044145; IF2_II.
DR InterPro; IPR006847; IF2_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR000178; TF_IF2_bacterial-like.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR43381; PTHR43381; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF11987; IF-2; 1.
DR Pfam; PF04760; IF2_N; 1.
DR SUPFAM; SSF50447; SSF50447; 2.
DR SUPFAM; SSF52156; SSF52156; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00487; IF-2; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51722; G_TR_2; 1.
DR PROSITE; PS01176; IF2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; GTP-binding; Initiation factor; Nucleotide-binding;
KW Protein biosynthesis.
FT CHAIN 1..856
FT /note="Translation initiation factor IF-2"
FT /id="PRO_0000228196"
FT DOMAIN 356..526
FT /note="tr-type G"
FT REGION 365..372
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 390..394
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 412..415
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 466..469
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 502..504
FT /note="G5"
FT /evidence="ECO:0000250"
FT BINDING 365..372
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT BINDING 412..416
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT BINDING 466..469
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ SEQUENCE 856 AA; 94986 MW; 8CD24371C22FBFA7 CRC64;
MNESKSVASN ELTSGKVERT TLKLSDKLKI SSNIQQGNKF SLNKSITTVE VRKSKKRKNI
DEAARSSLLL QNNDIDGNSE DKNSLTIQEQ ISRMNALQNA SNNEKREELS SDSNKHIEEE
VISVKAEVEQ SVDVVLPNDN LLIESDSSEK VIVDPVTDSE HGDKDFQDVV MLDEFVSSNL
DDAGDQKNGD QSDDISDLLE HKGIEGKKLK KYEKEHEEKK GNPKKVMSNN TYTKHVKLVI
EEELEEDNNK QVIKNYRSKK NRVTNRSVKN KITRKVLIPN KITVQELANS MSERVKDVQQ
VLYQMTGKHD IKLTDYLDSD QASIIVEAFN HTFKLVDNAK LENDLYSDGN NMELIPRAPV
VTVMGHVDHG KTSLLDAIRE SNVVDGEFKG ITQHIGAYQI TLNGDKKITF IDTPGHEAFA
AMRAHGTNVT DIVVLVVAAD DGIMPQTIES INHVKAANVA MIVAVNKIDK HDADLDRITN
ALLQHGVVAE SLGGDVIVVP VSAKEKINLD QLKSSILLIA DLLELKAVYN TRASGTVIES
KVDKNCGVVA TLIVQKGTLK VGDIIVAGNQ AYGRVRSMFN ADGGSEKVAI PSMPIKVFGL
NNVPNFGTSF IVVDSEKQAR ELINYRQDLL NVELSKQPAV DKSNILLYDM VDELNVILKC
DVMGSIEAIC YSIGKITHKD IRVNILYKGV GNITKSDVLL AETSNSIILA FNVKTDTQVK
ELAKQKNIEI KHYFVIYDII DDIKKILTGM LKPLKQEVQI GTLSVRKVFS VGNNGSVLGC
YVTSGLVKKG ALVKLVRNNN IIHEGKIKVL RRFKDDVKEV TAGFECGILL DYSKEIYPES
DVMNIFEIVE EIRVIQ
