ID   IF2_FRACC               Reviewed;        1062 AA.
AC   Q2J728;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN   Name=infB {ECO:0000255|HAMAP-Rule:MF_00100};
GN   OrderedLocusNames=Francci3_3562;
OS   Frankia casuarinae (strain DSM 45818 / CECT 9043 / CcI3).
OC   Bacteria; Actinobacteria; Frankiales; Frankiaceae; Frankia.
OX   NCBI_TaxID=106370;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 45818 / CECT 9043 / CcI3;
RX   PubMed=17151343; DOI=10.1101/gr.5798407;
RA   Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N.,
RA   Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A.,
RA   Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P.,
RA   Goltsman E., Huang Y., Kopp O.R., Labarre L., Lapidus A., Lavire C.,
RA   Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J.,
RA   Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F.,
RA   Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C.,
RA   Benson D.R.;
RT   "Genome characteristics of facultatively symbiotic Frankia sp. strains
RT   reflect host range and host plant biogeography.";
RL   Genome Res. 17:7-15(2007).
CC   -!- FUNCTION: One of the essential components for the initiation of protein
CC       synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis
CC       and promotes its binding to the 30S ribosomal subunits. Also involved
CC       in the hydrolysis of GTP during the formation of the 70S ribosomal
CC       complex. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00100}.
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DR   EMBL; CP000249; ABD12914.1; -; Genomic_DNA.
DR   RefSeq; WP_011437938.1; NZ_JENI01000005.1.
DR   AlphaFoldDB; Q2J728; -.
DR   SMR; Q2J728; -.
DR   STRING; 106370.Francci3_3562; -.
DR   EnsemblBacteria; ABD12914; ABD12914; Francci3_3562.
DR   KEGG; fra:Francci3_3562; -.
DR   eggNOG; COG0532; Bacteria.
DR   HOGENOM; CLU_006301_9_4_11; -.
DR   OMA; QVRPEMI; -.
DR   OrthoDB; 347113at2; -.
DR   PhylomeDB; Q2J728; -.
DR   Proteomes; UP000001937; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   CDD; cd03702; IF2_mtIF2_II; 1.
DR   Gene3D; 3.40.50.10050; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00100_B; IF_2_B; 1.
DR   InterPro; IPR044145; IF2_II.
DR   InterPro; IPR006847; IF2_N.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR000178; TF_IF2_bacterial-like.
DR   InterPro; IPR015760; TIF_IF2.
DR   InterPro; IPR023115; TIF_IF2_dom3.
DR   InterPro; IPR036925; TIF_IF2_dom3_sf.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   PANTHER; PTHR43381; PTHR43381; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF11987; IF-2; 1.
DR   Pfam; PF04760; IF2_N; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 2.
DR   SUPFAM; SSF52156; SSF52156; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00487; IF-2; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
DR   PROSITE; PS01176; IF2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; GTP-binding; Initiation factor; Nucleotide-binding;
KW   Protein biosynthesis; Reference proteome.
FT   CHAIN           1..1062
FT                   /note="Translation initiation factor IF-2"
FT                   /id="PRO_0000335472"
FT   DOMAIN          555..727
FT                   /note="tr-type G"
FT   REGION          34..463
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          564..571
FT                   /note="G1"
FT                   /evidence="ECO:0000250"
FT   REGION          589..593
FT                   /note="G2"
FT                   /evidence="ECO:0000250"
FT   REGION          614..617
FT                   /note="G3"
FT                   /evidence="ECO:0000250"
FT   REGION          668..671
FT                   /note="G4"
FT                   /evidence="ECO:0000250"
FT   REGION          704..706
FT                   /note="G5"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        71..124
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        125..139
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        149..200
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        214..228
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        254..282
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         564..571
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         614..618
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         668..671
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ   SEQUENCE   1062 AA;  107742 MW;  BAD86B2CB6058D77 CRC64;
     MAGKARVHEL AKELGVDSKT VLAKLKDLGE FVKSASSTVE APVVRKLKEA FPADGAPTSP
     GRATSRPGPP GGGARPTPPS RPGLAPRPGP RPVPGRPGPL GRPGPATPAP SPSPASPPLP
     ASPVQASPVQ ASPVQASPTS APAAPRPAAA SAVPAPPMPS VPSAPSGPRP GPNAPRPGAP
     QGGGRPRPGT PVPPSGPTAG GPTAGGPTAG GPTAGGPRPG PRPGPRSAAP GNNPYTTPSA
     GPRPSAGPRP SAGQSGPPST PTPRPSAPRS GTPRPGTPRP GGPRPGGTGA GGPRPTPGGM
     PPRPGGPRSG AGGGMPPRPG GTGGPRPNAN MFQPRPAGGP PGRPGGGGAP GRPGGGGAPG
     RPGGGGGPRP GGFAGRGGAP GRPGGGGGGG GAPGRPGGGG GGGGAPGRPA AGGRGRGGTT
     AGAFGPGGRG RPGRQRKSKR AKRQEWESGL EAPRMGAMVP RGNGQAIRLP RGASLADFAD
     KIDANPGALV QVVFTQLGEM VTATQSCTDE TLQLLGVTLG YEVQIVSPED EDKELLESFD
     LSFGGEYGDD VELSSRPPVV TVMGHVDHGK TKLLDAIRST DVVGGEAGGI TQHIGAYQVR
     AKVDGDERPI TFIDTPGHET FTAMRARGAQ VTDIVVLVVA ADDGVKPQTI EALNHAQAAG
     VPVVVAVNKV DKEGADPAKV RGQLTEYGLV AEEYGGDTMF VDVSARNRTN IDGLLEAIVL
     TADASLDLRA PTEVEAQGVA IEGRLDRGRG PVATVLVQRG TLRVGDSVVA GEAFGRVRAM
     LDEHGGQVVE AGPARPVQVL GFTSVPDAGD NFLVVPEDRV ARQIAERRQA RERNAELALS
     RGRPTLETIL ERMKEGEKTQ LNLILKGDVS GSVEALEDAL LKIDVGDEVG LRIIDRGVGA
     ITETNVMLAS ASDAIIIGFN VRPQGKATEL ADREGVEVRY YSVIYQAIED IENALKGMLK
     PVYEEAQLGT AEVREVFRVP RIGNVAGSLV RSGVIRRNTK ARLIRDGVVV ADNLTVESLK
     RFKDDATEVR EGYECGIGLG SFNDIKIDDV IETFEQREVP RA