APEB_PSEAE
ID APEB_PSEAE Reviewed; 429 AA.
AC Q9HYZ3;
DT 24-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Probable M18 family aminopeptidase 2;
DE EC=3.4.11.-;
GN Name=apeB; OrderedLocusNames=PA3247;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC -!- SIMILARITY: Belongs to the peptidase M18 family. {ECO:0000305}.
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DR EMBL; AE004091; AAG06635.1; -; Genomic_DNA.
DR PIR; A83240; A83240.
DR RefSeq; NP_251937.1; NC_002516.2.
DR RefSeq; WP_003114800.1; NZ_QZGE01000019.1.
DR PDB; 2IJZ; X-ray; 3.00 A; A/B/C/D/E/F/G/H/I/J/K/L=2-429.
DR PDB; 3WT4; X-ray; 2.30 A; A/B/C/D=1-429.
DR PDB; 4NJQ; X-ray; 2.70 A; A/B/C/D=1-429.
DR PDB; 4NJR; X-ray; 2.30 A; A/B/C/D=1-429.
DR PDB; 4OID; X-ray; 2.30 A; A/B/C/D=1-429.
DR PDB; 4OIW; X-ray; 2.44 A; A/B/C/D/E/F=1-429.
DR PDBsum; 2IJZ; -.
DR PDBsum; 3WT4; -.
DR PDBsum; 4NJQ; -.
DR PDBsum; 4NJR; -.
DR PDBsum; 4OID; -.
DR PDBsum; 4OIW; -.
DR AlphaFoldDB; Q9HYZ3; -.
DR SMR; Q9HYZ3; -.
DR STRING; 287.DR97_4688; -.
DR PaxDb; Q9HYZ3; -.
DR PRIDE; Q9HYZ3; -.
DR EnsemblBacteria; AAG06635; AAG06635; PA3247.
DR GeneID; 882410; -.
DR KEGG; pae:PA3247; -.
DR PATRIC; fig|208964.12.peg.3394; -.
DR PseudoCAP; PA3247; -.
DR HOGENOM; CLU_019532_2_0_6; -.
DR InParanoid; Q9HYZ3; -.
DR OMA; GPILKVN; -.
DR PhylomeDB; Q9HYZ3; -.
DR BioCyc; PAER208964:G1FZ6-3306-MON; -.
DR EvolutionaryTrace; Q9HYZ3; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-UniRule.
DR Gene3D; 2.30.250.10; -; 1.
DR HAMAP; MF_00467; Aminopeptidase_M18_2; 1.
DR InterPro; IPR022984; M18_aminopeptidase_2.
DR InterPro; IPR001948; Peptidase_M18.
DR InterPro; IPR023358; Peptidase_M18_dom2.
DR PANTHER; PTHR28570; PTHR28570; 1.
DR Pfam; PF02127; Peptidase_M18; 1.
DR PRINTS; PR00932; AMINO1PTASE.
PE 1: Evidence at protein level;
KW 3D-structure; Aminopeptidase; Hydrolase; Metal-binding; Metalloprotease;
KW Protease; Reference proteome; Zinc.
FT CHAIN 1..429
FT /note="Probable M18 family aminopeptidase 2"
FT /id="PRO_0000173465"
FT BINDING 82
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255"
FT BINDING 156
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255"
FT BINDING 401
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255"
FT HELIX 3..14
FT /evidence="ECO:0007829|PDB:3WT4"
FT HELIX 19..32
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 36..38
FT /evidence="ECO:0007829|PDB:4NJQ"
FT STRAND 51..56
FT /evidence="ECO:0007829|PDB:3WT4"
FT TURN 57..59
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 60..65
FT /evidence="ECO:0007829|PDB:3WT4"
FT HELIX 71..74
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 76..83
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 87..98
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 101..111
FT /evidence="ECO:0007829|PDB:3WT4"
FT HELIX 115..117
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 122..132
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 135..142
FT /evidence="ECO:0007829|PDB:3WT4"
FT HELIX 155..157
FT /evidence="ECO:0007829|PDB:3WT4"
FT HELIX 161..163
FT /evidence="ECO:0007829|PDB:3WT4"
FT HELIX 169..172
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 176..179
FT /evidence="ECO:0007829|PDB:3WT4"
FT HELIX 188..200
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 205..216
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 221..224
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 229..232
FT /evidence="ECO:0007829|PDB:3WT4"
FT HELIX 235..250
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 256..263
FT /evidence="ECO:0007829|PDB:3WT4"
FT HELIX 265..267
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 274..278
FT /evidence="ECO:0007829|PDB:3WT4"
FT HELIX 279..285
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 290..292
FT /evidence="ECO:0007829|PDB:4OID"
FT HELIX 293..298
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 302..306
FT /evidence="ECO:0007829|PDB:3WT4"
FT HELIX 317..319
FT /evidence="ECO:0007829|PDB:3WT4"
FT HELIX 322..324
FT /evidence="ECO:0007829|PDB:4NJQ"
FT STRAND 327..329
FT /evidence="ECO:0007829|PDB:2IJZ"
FT STRAND 333..335
FT /evidence="ECO:0007829|PDB:3WT4"
FT TURN 338..341
FT /evidence="ECO:0007829|PDB:3WT4"
FT HELIX 346..358
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 364..366
FT /evidence="ECO:0007829|PDB:3WT4"
FT HELIX 379..384
FT /evidence="ECO:0007829|PDB:3WT4"
FT TURN 385..387
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 390..394
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 396..399
FT /evidence="ECO:0007829|PDB:3WT4"
FT STRAND 402..408
FT /evidence="ECO:0007829|PDB:3WT4"
FT HELIX 411..423
FT /evidence="ECO:0007829|PDB:3WT4"
SQ SEQUENCE 429 AA; 46659 MW; FCBF80A93F67CBE3 CRC64;
MRAELNQGLI DFLKASPTPF HATASLARRL EAAGYRRLDE RDAWHTETGG RYYVTRNDSS
LIAIRLGRRS PLESGFRLVG AHTDSPCLRV KPNPEIARNG FLQLGVEVYG GALFAPWFDR
DLSLAGRVTF RANGKLESRL VDFRKAIAVI PNLAIHLNRA ANEGWPINAQ NELPPIIAQL
APGEAADFRL LLDEQLLREH GITADVVLDY ELSFYDTQSA AVVGLNDEFI AGARLDNLLS
CHAGLEALLN AEGDENCILV CTDHEEVGSC SHCGADGPFL EQVLRRLLPE GDAFSRAIQR
SLLVSADNAH GVHPNYADRH DANHGPALNG GPVIKINSNQ RYATNSETAG FFRHLCQDSE
VPVQSFVTRS DMGCGSTIGP ITASQVGVRT VDIGLPTFAM HSIRELAGSH DLAHLVKVLG
AFYASSELP
