IF2_MICAN
ID IF2_MICAN Reviewed; 1010 AA.
AC B0JU67;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=MAE_14330;
OS Microcystis aeruginosa (strain NIES-843 / IAM M-2473).
OC Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC Microcystaceae; Microcystis.
OX NCBI_TaxID=449447;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-843 / IAM M-247;
RX PubMed=18192279; DOI=10.1093/dnares/dsm026;
RA Kaneko T., Nakajima N., Okamoto S., Suzuki I., Tanabe Y., Tamaoki M.,
RA Nakamura Y., Kasai F., Watanabe A., Kawashima K., Kishida Y., Ono A.,
RA Shimizu Y., Takahashi C., Minami C., Fujishiro T., Kohara M., Katoh M.,
RA Nakazaki N., Nakayama S., Yamada M., Tabata S., Watanabe M.M.;
RT "Complete genomic structure of the bloom-forming toxic cyanobacterium
RT Microcystis aeruginosa NIES-843.";
RL DNA Res. 14:247-256(2007).
CC -!- FUNCTION: One of the essential components for the initiation of protein
CC synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis
CC and promotes its binding to the 30S ribosomal subunits. Also involved
CC in the hydrolysis of GTP during the formation of the 70S ribosomal
CC complex. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00100}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00100}.
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DR EMBL; AP009552; BAG01255.1; -; Genomic_DNA.
DR RefSeq; WP_012264842.1; NC_010296.1.
DR AlphaFoldDB; B0JU67; -.
DR SMR; B0JU67; -.
DR STRING; 449447.MAE_14330; -.
DR PaxDb; B0JU67; -.
DR EnsemblBacteria; BAG01255; BAG01255; MAE_14330.
DR KEGG; mar:MAE_14330; -.
DR PATRIC; fig|449447.4.peg.1314; -.
DR eggNOG; COG0532; Bacteria.
DR eggNOG; COG3170; Bacteria.
DR HOGENOM; CLU_006301_5_1_3; -.
DR OMA; NIAVKSH; -.
DR OrthoDB; 347113at2; -.
DR BioCyc; MAER449447:MAE_RS06305-MON; -.
DR Proteomes; UP000001510; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR CDD; cd03702; IF2_mtIF2_II; 1.
DR Gene3D; 3.40.50.10050; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00100_B; IF_2_B; 1.
DR InterPro; IPR044145; IF2_II.
DR InterPro; IPR006847; IF2_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR000178; TF_IF2_bacterial-like.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR43381; PTHR43381; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF11987; IF-2; 1.
DR Pfam; PF04760; IF2_N; 2.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50447; SSF50447; 2.
DR SUPFAM; SSF52156; SSF52156; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00487; IF-2; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51722; G_TR_2; 1.
DR PROSITE; PS01176; IF2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; GTP-binding; Initiation factor; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome.
FT CHAIN 1..1010
FT /note="Translation initiation factor IF-2"
FT /id="PRO_1000075608"
FT DOMAIN 502..675
FT /note="tr-type G"
FT REGION 54..350
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 364..420
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 511..518
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 536..540
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 561..564
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 615..618
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 651..653
FT /note="G5"
FT /evidence="ECO:0000250"
FT COMPBIAS 56..70
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 71..94
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 163..177
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 194..258
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 273..287
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 315..333
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 511..518
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT BINDING 561..565
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT BINDING 615..618
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ SEQUENCE 1010 AA; 109510 MW; A886B7BEDB268859 CRC64;
MSNTKVRIYD LSKELNLDNK DILDICDQLN IEYKSHSSTI SEEDAQRIKA IAAKGLASST
SKNSTGQRES ASPAEEKKQK ILALHKHNRP ETGEEGESYP GQAGSSAKPT LISPPRPPVK
PLVAPPGRDE AAEKTPPTAA EMLSHSPSVK ETPTETPLVP EAAPTLIAPP NRPSLTPKPR
PETASGRPEP RSKNAPGSND RPRGEKRERG ESENAPSPER RVGLAKPEKP TLNRKPDGKS
PKLAEPAREV RETVELKRPV RPGLPAKISA TSEEETDTTK KSGVDGTEID TDTGLLGADG
PKKLKRPTMP PRMAKKSTWE EEEEEEKKAA KTAKTAGKNK RRTQALFEDD DDLESELSGL
INTPSFTLST ARPPKPPTAK AAPPGTPTAV KVKRPSKPTA HTGSPKSERQ EPQEEKRPES
IVVTGSLTVR DLSELMKVPE TEIIRTLFFK GMAVNITQTL DVDTIEMIAR DFEIAVETPS
TQSAAIKTTE MIDVSDWESL QRRPPVVTIM GHVDHGKTTL LDSIRKTKVA QGEAGGITQH
IGAYHVDVEH NGKPEQIVFL DTPGHEAFTA MRARGARVTD IAVLVVAADD GVQPQTKEAI
SHARAAEVPI VVAINKVDKP SANPDRIKQE LTEQGLVAED WGGETIMVPV SALRGENLDN
LLEMILLVAE VEELVANPDR LAKGTVIEAN LDRTKGPVAT LLVQNGTLRV GDSIVAGSVF
GKIRAMIDDR GQKVEAATPS FAVEILGLSD VPAAGDEFDV YESEKEARSI ADQRAIERRN
TRLQQALSSR RVSLSTLSIQ AQEGQLKELN LILKADVQGS VEAILGALKQ LPQNEVQIRV
LLASPGEITE TDVDLAAASG AVVVGFNTTL ASGARASADR EGVDIRDYNI IYKLLDDIQG
AMEGLLDPEE VEEHLGFAEV RAVFTVGRGA VAGCYVQSGK LVRNRFLRVR RGKEIVYQGV
LDSLKRMKED AREVATGFEC GVGVSKFNDW KEGDIIEAYE MVMKRRTLSS
