IF2_MYCGE
ID IF2_MYCGE Reviewed; 619 AA.
AC P47388;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Translation initiation factor IF-2;
GN Name=infB; OrderedLocusNames=MG142;
OS Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS (Mycoplasmoides genitalium).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=243273;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT "The minimal gene complement of Mycoplasma genitalium.";
RL Science 270:397-403(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 89-131.
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=8253680; DOI=10.1128/jb.175.24.7918-7930.1993;
RA Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT "A survey of the Mycoplasma genitalium genome by using random sequencing.";
RL J. Bacteriol. 175:7918-7930(1993).
CC -!- FUNCTION: One of the essential components for the initiation of protein
CC synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis
CC and promotes its binding to the 30S ribosomal subunits. Also involved
CC in the hydrolysis of GTP during the formation of the 70S ribosomal
CC complex (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000305}.
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DR EMBL; L43967; AAC71360.1; -; Genomic_DNA.
DR EMBL; U01765; AAD10581.1; -; Genomic_DNA.
DR PIR; G64215; G64215.
DR RefSeq; WP_010869352.1; NC_000908.2.
DR AlphaFoldDB; P47388; -.
DR SMR; P47388; -.
DR STRING; 243273.MG_142; -.
DR EnsemblBacteria; AAC71360; AAC71360; MG_142.
DR KEGG; mge:MG_142; -.
DR eggNOG; COG0532; Bacteria.
DR HOGENOM; CLU_006301_5_1_14; -.
DR OMA; NRDNRTG; -.
DR OrthoDB; 347113at2; -.
DR BioCyc; MGEN243273:G1GJ2-165-MON; -.
DR Proteomes; UP000000807; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR GO; GO:0006413; P:translational initiation; IBA:GO_Central.
DR CDD; cd03702; IF2_mtIF2_II; 1.
DR Gene3D; 3.40.50.10050; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00100_B; IF_2_B; 1.
DR InterPro; IPR044145; IF2_II.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR000178; TF_IF2_bacterial-like.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR43381; PTHR43381; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF11987; IF-2; 1.
DR SUPFAM; SSF50447; SSF50447; 2.
DR SUPFAM; SSF52156; SSF52156; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00487; IF-2; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51722; G_TR_2; 1.
DR PROSITE; PS01176; IF2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; GTP-binding; Initiation factor; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome.
FT CHAIN 1..619
FT /note="Translation initiation factor IF-2"
FT /id="PRO_0000137221"
FT DOMAIN 120..289
FT /note="tr-type G"
FT REGION 129..136
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 154..158
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 176..179
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 230..233
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 266..268
FT /note="G5"
FT /evidence="ECO:0000250"
FT BINDING 129..136
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 176..180
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 230..233
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
SQ SEQUENCE 619 AA; 67888 MW; 3CD52DC8247808EE CRC64;
MKKNRAFNQV KKTKFDGRIK TSAKHQLRNV KTGVKDGVFI YKGPLTVSEF ASKTNIAVAN
IIKHFFLNGL ALTVNSVLTN EQLADACVNF GFDFKMETEV THENIVANIQ FEDSDDLLQP
RPPIVTIMGH VDHGKTSLLD TIRKTNVTAK EFGGITQKIG AYQVKNHQNK TITFIDTPGH
EAFTLMRARG AKVTDIVVLV VAADDGIKKQ TEEAISHAKS ANTPIIVFIN KMDKPTANPD
LVIQQLNKFD LVPEAWGGKT IFVMGSALTG QGINELLDNI LLLGEVEGYQ ANYNAHSSGY
AIEVQTSKGL GPIANVIVKR GTLKLGDIVV LGPAYGRVRT MHDENGNSLK QATPSKPVQI
SGFDIMPVAG EKFIVFDDEK DAKLIANKFK EQQKQKANNL TVNQTLKEQI KNKEIKILNL
IFKADSDGSL QAIKQAVENI NVAKISLSII HAAVGQISES DIMLAKASGA LLFSLNLGLS
QTVKNIASLQ GVKLEVHYHI PKLAEEIENI LKGQLDPVYE EIEIGKAEVL QLWFHSKIGN
IAGTIVKSGK IKRGNLCKLF RDKEIIFEGR IDSLKNEKTP VNLIETGKNC GIVINGCNDI
KIGDIIVAYE KQIVKDGKL
