IF2_NOSP7
ID IF2_NOSP7 Reviewed; 1056 AA.
AC B2J955;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=Npun_R5773;
OS Nostoc punctiforme (strain ATCC 29133 / PCC 73102).
OC Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=63737;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29133 / PCC 73102;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C., Elhai J.,
RA Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R.;
RT "Complete sequence of chromosome of Nostoc punctiforme ATCC 29133.";
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: One of the essential components for the initiation of protein
CC synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis
CC and promotes its binding to the 30S ribosomal subunits. Also involved
CC in the hydrolysis of GTP during the formation of the 70S ribosomal
CC complex. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00100}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00100}.
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DR EMBL; CP001037; ACC84071.1; -; Genomic_DNA.
DR RefSeq; WP_012412014.1; NC_010628.1.
DR AlphaFoldDB; B2J955; -.
DR SMR; B2J955; -.
DR STRING; 63737.Npun_R5773; -.
DR EnsemblBacteria; ACC84071; ACC84071; Npun_R5773.
DR KEGG; npu:Npun_R5773; -.
DR eggNOG; COG0532; Bacteria.
DR eggNOG; COG3266; Bacteria.
DR HOGENOM; CLU_006301_7_0_3; -.
DR OMA; NIAVKSH; -.
DR OrthoDB; 347113at2; -.
DR PhylomeDB; B2J955; -.
DR Proteomes; UP000001191; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR CDD; cd03702; IF2_mtIF2_II; 1.
DR Gene3D; 3.40.50.10050; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00100_B; IF_2_B; 1.
DR InterPro; IPR044145; IF2_II.
DR InterPro; IPR006847; IF2_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR000178; TF_IF2_bacterial-like.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR43381; PTHR43381; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF11987; IF-2; 1.
DR Pfam; PF04760; IF2_N; 2.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50447; SSF50447; 2.
DR SUPFAM; SSF52156; SSF52156; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00487; IF-2; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51722; G_TR_2; 1.
DR PROSITE; PS01176; IF2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; GTP-binding; Initiation factor; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome.
FT CHAIN 1..1056
FT /note="Translation initiation factor IF-2"
FT /id="PRO_1000093808"
FT DOMAIN 550..723
FT /note="tr-type G"
FT REGION 60..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 109..359
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 406..472
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 559..566
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 584..588
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 609..612
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 663..666
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 699..701
FT /note="G5"
FT /evidence="ECO:0000250"
FT COMPBIAS 62..77
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 109..124
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 125..146
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 149..177
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 228..253
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 257..271
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 442..469
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 559..566
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT BINDING 609..613
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT BINDING 663..666
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ SEQUENCE 1056 AA; 114029 MW; D461C43D67DF72E1 CRC64;
MTNGKVRIYE LSKELNLDNK ELLAICDQLS IAVKSHSSTI SESEAENIRT AAEKLAATNV
SAKKELGTTS HKPNSPPNGG RNRPAAPHKQ QILEIRKPKI LRNTTSNAPE ASVANNTQAA
LSEANPPSPP RPFATPASPM KPAAPTRPVP RTQSETQEQP PVTDLEQTPN PSQAPEKVAS
QKPEKVVPPR PKSEKPQKPQ LVAPPARPAA EVAQAEVAQV SDEPVSQADK PILKRDQRLR
PVEGDREQIK PRVAKLPTDQ SPQSAPQRQA RPTPAPTRPE QRGNRPSAPS QLGEGQRPRP
ARPGESVAAA MPIATPPRQM SGIAGKSQGL GDEPVTPDLL DLKRPSPPRP TKGGKKWVEE
EIIDEVKEKA KAGVKGKRIK PILDDEFEED LLDDDDIDSP ATVQVSLSIA RPPKPKATRP
VQMPGASLAS APTARGSRKP GSKSGSNRDH HNNRRQQEAD TKRDRPEKVT ITGPLTVQEL
SDVLAVPDTE IVKILFLKGM AVSITQNLDI PTITLVGKEL EIEVETVERE AEARKITEMV
GAEDQEYLHR RPPVVTIMGH VDHGKTTLLD SIRKTKVAAG EAGGITQHIG AYHVDVVHEG
KPQQIVFLDT PGHEAFTAMR ARGARVTDIA VLVVAADDGV RPQTIEAISH AQAAGVPIVV
AINKIDKEGA QPERVKQELT QYGLTAEDWG GETIMVPVSA IRGENLDTLL EMILLVAEVG
ELSANPDRTA KGTVIEAHLD KAKGAVATLL IQNGTLHVGD ILVAGSAFGK VRAMVDDRGK
RVDIASPSFA VEVLGLSDVP AAGDEFEVFQ NEKEARSLAS DRADRQRLSR LLQGRVTLTT
LSAQAQEGEL KELNLILKGD VQGSVEAIVG ALKQIPQNEV QIRMLLATAG EITETDIDLA
AASNAVIIGF NTTFASGARQ AADEAGVDVR EYNVIYKLLE DIQDALEGLL EPELVEEPLG
QTEVRAVFPV GRGAVAGCYV QSGKLVRNCK VRVRRGGKVI YEGVLDSLKR MKEDAREVNA
GYECGVGMDK FNDWVEGDII ESYQMVTKRR TLTLTR
