APEH_AERPE
ID APEH_AERPE Reviewed; 582 AA.
AC Q9YBQ2;
DT 07-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Acylamino-acid-releasing enzyme;
DE Short=AARE;
DE EC=3.4.19.1;
DE AltName: Full=Acyl-peptide hydrolase;
DE Short=APH;
DE AltName: Full=Acylaminoacyl-peptidase;
GN OrderedLocusNames=APE_1547.1;
OS Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 /
OS K1).
OC Archaea; Crenarchaeota; Thermoprotei; Desulfurococcales;
OC Desulfurococcaceae; Aeropyrum.
OX NCBI_TaxID=272557;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1;
RX PubMed=10382966; DOI=10.1093/dnares/6.2.83;
RA Kawarabayasi Y., Hino Y., Horikawa H., Yamazaki S., Haikawa Y., Jin-no K.,
RA Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A.,
RA Fukui S., Nagai Y., Nishijima K., Nakazawa H., Takamiya M., Masuda S.,
RA Funahashi T., Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A.,
RA Aoki K., Kubota K., Nakamura Y., Nomura N., Sako Y., Kikuchi H.;
RT "Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon,
RT Aeropyrum pernix K1.";
RL DNA Res. 6:83-101(1999).
RN [2]
RP CRYSTALLIZATION.
RC STRAIN=ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1;
RX PubMed=12037315; DOI=10.1107/s0907444902005875;
RA Wang G., Gao R., Ding Y., Yang H., Cao S., Feng Y., Rao Z.;
RT "Crystallization and preliminary crystallographic analysis of acylamino-
RT acid releasing enzyme from the hyperthermophilic archaeon Aeropyrum
RT pernix.";
RL Acta Crystallogr. D 58:1054-1055(2002).
CC -!- FUNCTION: This enzyme catalyzes the hydrolysis of the N-terminal
CC peptide bond of an N-acetylated peptide to generate an N-acetylated
CC amino acid and a peptide with a free N-terminus.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Cleavage of an N-acetyl or N-formyl amino acid from the N-
CC terminus of a polypeptide.; EC=3.4.19.1;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the peptidase S9C family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA80546.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BA000002; BAA80546.2; ALT_INIT; Genomic_DNA.
DR PIR; D72636; D72636.
DR PDB; 1VE6; X-ray; 2.10 A; A/B=1-582.
DR PDB; 1VE7; X-ray; 2.70 A; A/B=1-582.
DR PDB; 2HU5; X-ray; 2.00 A; A/B=1-582.
DR PDB; 2HU7; X-ray; 2.01 A; A/B=1-582.
DR PDB; 2HU8; X-ray; 2.40 A; A/B=1-582.
DR PDB; 2QR5; X-ray; 2.20 A; A/B=1-582.
DR PDB; 2QZP; X-ray; 2.70 A; A/B=21-582.
DR PDB; 3O4G; X-ray; 2.50 A; A/B/C/D=1-582.
DR PDB; 3O4H; X-ray; 1.82 A; A/B/C/D=1-582.
DR PDB; 3O4I; X-ray; 2.70 A; A/B=1-582.
DR PDB; 3O4J; X-ray; 2.50 A; A/B/C/D=1-582.
DR PDB; 4RE5; X-ray; 1.90 A; A/B=1-582.
DR PDB; 4RE6; X-ray; 2.55 A; A/B/C/D=1-582.
DR PDBsum; 1VE6; -.
DR PDBsum; 1VE7; -.
DR PDBsum; 2HU5; -.
DR PDBsum; 2HU7; -.
DR PDBsum; 2HU8; -.
DR PDBsum; 2QR5; -.
DR PDBsum; 2QZP; -.
DR PDBsum; 3O4G; -.
DR PDBsum; 3O4H; -.
DR PDBsum; 3O4I; -.
DR PDBsum; 3O4J; -.
DR PDBsum; 4RE5; -.
DR PDBsum; 4RE6; -.
DR AlphaFoldDB; Q9YBQ2; -.
DR SMR; Q9YBQ2; -.
DR STRING; 272557.APE_1547.1; -.
DR ESTHER; aerpe-APE1547; ACPH_Peptidase_S9.
DR MEROPS; S09.070; -.
DR EnsemblBacteria; BAA80546; BAA80546; APE_1547.1.
DR KEGG; ape:APE_1547.1; -.
DR eggNOG; arCOG01646; Archaea.
DR OMA; DIGDPGG; -.
DR BRENDA; 3.1.1.1; 171.
DR BRENDA; 3.4.19.1; 171.
DR EvolutionaryTrace; Q9YBQ2; -.
DR Proteomes; UP000002518; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR001375; Peptidase_S9.
DR Pfam; PF00326; Peptidase_S9; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Hydrolase; Reference proteome.
FT CHAIN 1..582
FT /note="Acylamino-acid-releasing enzyme"
FT /id="PRO_0000122436"
FT ACT_SITE 445
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 524
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 556
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT HELIX 9..21
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 24..31
FT /evidence="ECO:0007829|PDB:3O4H"
FT TURN 32..34
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 35..42
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 45..52
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 55..58
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 65..67
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 75..82
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 90..96
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 103..105
FT /evidence="ECO:0007829|PDB:4RE5"
FT STRAND 111..119
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 124..129
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 134..140
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 143..152
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 154..160
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 163..171
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 174..181
FT /evidence="ECO:0007829|PDB:3O4H"
FT TURN 182..184
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 188..190
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 196..202
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 208..213
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 218..223
FT /evidence="ECO:0007829|PDB:3O4H"
FT TURN 225..227
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 230..232
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 236..238
FT /evidence="ECO:0007829|PDB:4RE5"
FT HELIX 239..243
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 246..253
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 255..257
FT /evidence="ECO:0007829|PDB:2QZP"
FT STRAND 259..265
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 268..273
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 276..278
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 282..291
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 294..301
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 304..311
FT /evidence="ECO:0007829|PDB:3O4H"
FT TURN 312..314
FT /evidence="ECO:0007829|PDB:2QR5"
FT STRAND 316..318
FT /evidence="ECO:0007829|PDB:3O4H"
FT HELIX 324..328
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 330..339
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 345..353
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 358..366
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 368..371
FT /evidence="ECO:0007829|PDB:3O4H"
FT HELIX 380..387
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 391..395
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 401..403
FT /evidence="ECO:0007829|PDB:3O4H"
FT HELIX 405..409
FT /evidence="ECO:0007829|PDB:3O4H"
FT TURN 410..413
FT /evidence="ECO:0007829|PDB:3O4H"
FT TURN 415..417
FT /evidence="ECO:0007829|PDB:3O4H"
FT HELIX 418..432
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 436..444
FT /evidence="ECO:0007829|PDB:3O4H"
FT HELIX 446..457
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 461..463
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 465..469
FT /evidence="ECO:0007829|PDB:3O4H"
FT HELIX 474..479
FT /evidence="ECO:0007829|PDB:3O4H"
FT HELIX 483..492
FT /evidence="ECO:0007829|PDB:3O4H"
FT TURN 493..495
FT /evidence="ECO:0007829|PDB:3O4H"
FT HELIX 497..502
FT /evidence="ECO:0007829|PDB:3O4H"
FT HELIX 505..511
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 516..521
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 525..527
FT /evidence="ECO:0007829|PDB:3O4H"
FT HELIX 529..541
FT /evidence="ECO:0007829|PDB:3O4H"
FT STRAND 546..551
FT /evidence="ECO:0007829|PDB:3O4H"
FT HELIX 561..579
FT /evidence="ECO:0007829|PDB:3O4H"
SQ SEQUENCE 582 AA; 63035 MW; A9F2DD39ACD1C3DF CRC64;
MRIIMPVEFS RIVRDVERLI AVEKYSLQGV VDGDKLLVVG FSEGSVNAYL YDGGETVKLN
REPINSVLDP HYGVGRVILV RDVSKGAEQH ALFKVNTSRP GEEQRLEAVK PMRILSGVDT
GEAVVFTGAT EDRVALYALD GGGLRELARL PGFGFVSDIR GDLIAGLGFF GGGRVSLFTS
NLSSGGLRVF DSGEGSFSSA SISPGMKVTA GLETAREARL VTVDPRDGSV EDLELPSKDF
SSYRPTAITW LGYLPDGRLA VVARREGRSA VFIDGERVEA PQGNHGRVVL WRGKLVTSHT
SLSTPPRIVS LPSGEPLLEG GLPEDLRRSI AGSRLVWVES FDGSRVPTYV LESGRAPTPG
PTVVLVHGGP FAEDSDSWDT FAASLAAAGF HVVMPNYRGS TGYGEEWRLK IIGDPCGGEL
EDVSAAARWA RESGLASELY IMGYSYGGYM TLCALTMKPG LFKAGVAGAS VVDWEEMYEL
SDAAFRNFIE QLTGGSREIM RSRSPINHVD RIKEPLALIH PQNDSRTPLK PLLRLMGELL
ARGKTFEAHI IPDAGHAINT MEDAVKILLP AVFFLATQRE RR
