ID   IF2_RHOE4               Reviewed;         981 AA.
AC   C0ZYA5;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   26-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN   Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=RER_26320;
OS   Rhodococcus erythropolis (strain PR4 / NBRC 100887).
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Rhodococcus;
OC   Rhodococcus erythropolis group.
OX   NCBI_TaxID=234621;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PR4 / NBRC 100887;
RA   Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S.,
RA   Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.;
RT   "Comparison of the complete genome sequences of Rhodococcus erythropolis
RT   PR4 and Rhodococcus opacus B4.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: One of the essential components for the initiation of protein
CC       synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis
CC       and promotes its binding to the 30S ribosomal subunits. Also involved
CC       in the hydrolysis of GTP during the formation of the 70S ribosomal
CC       complex. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00100}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP008957; BAH33340.1; -; Genomic_DNA.
DR   RefSeq; WP_020907439.1; NC_012490.1.
DR   AlphaFoldDB; C0ZYA5; -.
DR   SMR; C0ZYA5; -.
DR   STRING; 234621.RER_26320; -.
DR   PRIDE; C0ZYA5; -.
DR   EnsemblBacteria; BAH33340; BAH33340; RER_26320.
DR   KEGG; rer:RER_26320; -.
DR   eggNOG; COG0481; Bacteria.
DR   eggNOG; COG0532; Bacteria.
DR   HOGENOM; CLU_006301_9_3_11; -.
DR   OMA; QVRPEMI; -.
DR   Proteomes; UP000002204; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   CDD; cd03702; IF2_mtIF2_II; 1.
DR   Gene3D; 3.40.50.10050; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00100_B; IF_2_B; 1.
DR   InterPro; IPR044145; IF2_II.
DR   InterPro; IPR006847; IF2_N.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR000178; TF_IF2_bacterial-like.
DR   InterPro; IPR015760; TIF_IF2.
DR   InterPro; IPR023115; TIF_IF2_dom3.
DR   InterPro; IPR036925; TIF_IF2_dom3_sf.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   PANTHER; PTHR43381; PTHR43381; 1.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF11987; IF-2; 1.
DR   Pfam; PF04760; IF2_N; 2.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 2.
DR   SUPFAM; SSF52156; SSF52156; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00487; IF-2; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; GTP-binding; Initiation factor; Nucleotide-binding;
KW   Protein biosynthesis.
FT   CHAIN           1..981
FT                   /note="Translation initiation factor IF-2"
FT                   /id="PRO_1000202783"
FT   DOMAIN          477..649
FT                   /note="tr-type G"
FT   REGION          31..370
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          486..493
FT                   /note="G1"
FT                   /evidence="ECO:0000250"
FT   REGION          511..515
FT                   /note="G2"
FT                   /evidence="ECO:0000250"
FT   REGION          536..539
FT                   /note="G3"
FT                   /evidence="ECO:0000250"
FT   REGION          590..593
FT                   /note="G4"
FT                   /evidence="ECO:0000250"
FT   REGION          626..628
FT                   /note="G5"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        82..151
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        216..273
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         486..493
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         536..540
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT   BINDING         590..593
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ   SEQUENCE   981 AA;  100446 MW;  BA5ACAECE311901B CRC64;
     MAGKARVHEL AKELGVTSKE LLATLKEQGE FVKSASSTVE APVARRLRES FPSAGGAETK
     SETGAAAPAA RPAAKPGAPS PSAAKPGGPR PGPKPAAPAP AAPAAPAPAA PAAPAAAAPA
     APSAPVPTPT FNAPKPAQPA RPAPAAPAAS APAAPAAPAA PSTGAKPGGP RPGPKAPRVG
     NNPYSSAPAE RPAPRPAPGA PRPGAGQGGS RPAPGQGGPR PAPGQGGPRP APGQGGPRPA
     PGQGGPRPPA GQGGPRPSPG SMPPRPNPGA MPARSARPAP GGGGRPGRPG GAPGGRPGGG
     GGGYRGGGAP GAGAGAGAPG GAAPAGGFRG RPGGGGRPGQ RGAAAGAFGR PGGAVRRGRK
     SKRAKRAEYE SMQAPAVGGV RLPRGNGETI RLARGASLSD FADKIDANPA ALVQALFNLG
     EMVTATQSVN DETLELLGGE MNYVVQVVSP EDEDRELLDS FDLTYGEDAG GEEDLESRPP
     VVTVMGHVDH GKTRLLDVIR KANVREGEAG GITQHIGAYQ VLTELEGNER LVTFIDTPGH
     EAFTAMRARG AKATDLAILV VAADDGVMPQ TVEAINHAQA ADVPIVVAVN KIDKEGANPD
     KIRQQLTEYG LVAEEYGGDT MFVDISAKQG LNIDALLEAV LLTADASLDL RANPDMDAQG
     VAIEAHLDRG RGPVATVLIQ RGTLRVGDSI VAGDAYGRVR RMVDEHGQDV HEALPSRPVQ
     VIGFTSVPGA GDNLLVVDED RIARQIADRR NARKRNALAA KSRKRISLDD LDAALKEHSQ
     LNLILKGDNS GTVEALEEAL LGIPIDDEVQ LRVIDRGVGG ITETNVNLAA ASNAIIIGFN
     VRAEGKATEL ANREGVDIRY YSVIYQAIDE VEKALKGLLK PVYEEVELGK AEIRAMFRSS
     KIGNIAGCLV TSGSIRRNAK ARLIRDSKVI AETVTISSLK REKEDATEVR EGYECGLTVT
     YSDIKIGDVL ECYELREKPR D