IF2_SOLUE
ID IF2_SOLUE Reviewed; 1001 AA.
AC Q01W31;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=Acid_5181;
OS Solibacter usitatus (strain Ellin6076).
OC Bacteria; Acidobacteria; Bryobacterales; Solibacteraceae;
OC Candidatus Solibacter.
OX NCBI_TaxID=234267;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ellin6076;
RX PubMed=19201974; DOI=10.1128/aem.02294-08;
RA Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M.,
RA Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B.,
RA Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C.,
RA Davidsen T.M., DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S.,
RA Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P.,
RA Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q.,
RA Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R.,
RA Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R.;
RT "Three genomes from the phylum Acidobacteria provide insight into the
RT lifestyles of these microorganisms in soils.";
RL Appl. Environ. Microbiol. 75:2046-2056(2009).
CC -!- FUNCTION: One of the essential components for the initiation of protein
CC synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis
CC and promotes its binding to the 30S ribosomal subunits. Also involved
CC in the hydrolysis of GTP during the formation of the 70S ribosomal
CC complex. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00100}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00100}.
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DR EMBL; CP000473; ABJ86134.1; -; Genomic_DNA.
DR RefSeq; WP_011686879.1; NC_008536.1.
DR AlphaFoldDB; Q01W31; -.
DR SMR; Q01W31; -.
DR STRING; 234267.Acid_5181; -.
DR PRIDE; Q01W31; -.
DR EnsemblBacteria; ABJ86134; ABJ86134; Acid_5181.
DR KEGG; sus:Acid_5181; -.
DR eggNOG; COG0532; Bacteria.
DR eggNOG; COG3266; Bacteria.
DR HOGENOM; CLU_006301_5_1_0; -.
DR OMA; QVRPEMI; -.
DR OrthoDB; 347113at2; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR CDD; cd03702; IF2_mtIF2_II; 1.
DR Gene3D; 3.40.50.10050; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00100_B; IF_2_B; 1.
DR InterPro; IPR044145; IF2_II.
DR InterPro; IPR006847; IF2_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR000178; TF_IF2_bacterial-like.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR43381; PTHR43381; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF11987; IF-2; 1.
DR Pfam; PF04760; IF2_N; 2.
DR SUPFAM; SSF50447; SSF50447; 2.
DR SUPFAM; SSF52156; SSF52156; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00487; IF-2; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51722; G_TR_2; 1.
DR PROSITE; PS01176; IF2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; GTP-binding; Initiation factor; Nucleotide-binding;
KW Protein biosynthesis.
FT CHAIN 1..1001
FT /note="Translation initiation factor IF-2"
FT /id="PRO_1000008342"
FT DOMAIN 499..668
FT /note="tr-type G"
FT REGION 56..418
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 508..515
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 533..537
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 554..557
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 608..611
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 644..646
FT /note="G5"
FT /evidence="ECO:0000250"
FT COMPBIAS 60..87
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 119..215
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 230..256
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 301..328
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 341..355
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 380..407
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 508..515
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT BINDING 554..558
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT BINDING 608..611
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ SEQUENCE 1001 AA; 107507 MW; AEB82602AA7AD11B CRC64;
MKKIRINELA RELEVKAHEI LERLPELGVT EKKTHSSSID EDVAIKLRQY YGQDVPDYVH
DPNAVDEPAT EAHEERHEHE EAHEPAAAPK AAVEPETPVA PAPEAAPAAK EERPAPEEPA
RPMAPIRPPL ASGRPIHPPV GATPPPRPEG PRAAPAPPLP PPAPQVPHAP SPAASSPAMP
ARPEPPAHHP PSQTPPAAVF APGRPAPPSA KPLPTTTPRP GQVLSGPRQP FPSSPAPGAP
QRPQAIPRPP QQVRPESQRP SGPGAPSAPQ RPLAGQPAAR PVVPPRPDLV AKLSAPRAPV
MPQQPAAPRP GVPKAPSAPV PGQPIYRGPI RPGQPMVAKP GVRPGMPPSR PGGPRPQHPT
SRGRIEPGMG APPPPAEPSR GRPGDRRPVR QQRERTEEEK ILRPQRRHVE AGPPPISREI
TISEGITVKE LSEKLDVKAN LVMKKLMDRG IFVAINQTLD GKLATEVARD FGASTATVSY
EVEAMQSVEE AQDTTDLERR APVVTIMGHV DHGKTSLLDA IREANVAGRE AGGITQHIGA
YQVEMKGRKI VFIDTPGHEA FTRMRSRGAK VTDIVILVVA ADDGVMPQTL EAIDHAKAAA
VPIIVAINKI DKADAQPERI KQQLSDRGLL PEDWGGDVVM VPVSARTQQG LPDLLEMILL
VADMQDLKAN PSRPAMGTVI EAQLDRGRGP VATVLVRNGT LSVGDFFICG AVFGKVRAML
NDRGTQIRKA EPSTPVEVLG LDSLPEAGDD FQVVTDTAKA KQIVNFRDQR QKEAALAKSS
RITLEQLHQQ MREGEVKELP VIIKADVGGS AEVLKETLEK LSNDKVKVRV IHSGVGAINE
SDILLASASN AIVIGFNVRP ERNAAATAEQ EKVDVRLHTI IYNLTDEIKR AMSGLLAPVF
KEVYRGKAEV RETFRISKVG AVAGCQVIDG SIPRDSECRV LRDNIVVHTG KIGSLRRFKD
DVSEVKIGME CGITLANFAD LKQGDIIEAF ATERVATEVF A
