IF2_STRAW
ID IF2_STRAW Reviewed; 1046 AA.
AC Q82K53;
DT 15-DEC-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Translation initiation factor IF-2 {ECO:0000255|HAMAP-Rule:MF_00100};
GN Name=infB {ECO:0000255|HAMAP-Rule:MF_00100}; OrderedLocusNames=SAV_2551;
OS Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC
OS 14893 / NCIMB 12804 / NRRL 8165 / MA-4680).
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces.
OX NCBI_TaxID=227882;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL
RC 8165 / MA-4680;
RX PubMed=11572948; DOI=10.1073/pnas.211433198;
RA Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M.,
RA Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T.,
RA Sakaki Y., Hattori M.;
RT "Genome sequence of an industrial microorganism Streptomyces avermitilis:
RT deducing the ability of producing secondary metabolites.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL
RC 8165 / MA-4680;
RX PubMed=12692562; DOI=10.1038/nbt820;
RA Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T.,
RA Sakaki Y., Hattori M., Omura S.;
RT "Complete genome sequence and comparative analysis of the industrial
RT microorganism Streptomyces avermitilis.";
RL Nat. Biotechnol. 21:526-531(2003).
CC -!- FUNCTION: One of the essential components for the initiation of protein
CC synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis
CC and promotes its binding to the 30S ribosomal subunits. Also involved
CC in the hydrolysis of GTP during the formation of the 70S ribosomal
CC complex. {ECO:0000255|HAMAP-Rule:MF_00100}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00100}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00100}.
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DR EMBL; BA000030; BAC70262.1; -; Genomic_DNA.
DR AlphaFoldDB; Q82K53; -.
DR SMR; Q82K53; -.
DR STRING; 227882.SAV_2551; -.
DR EnsemblBacteria; BAC70262; BAC70262; SAVERM_2551.
DR KEGG; sma:SAVERM_2551; -.
DR eggNOG; COG0532; Bacteria.
DR HOGENOM; CLU_006301_9_4_11; -.
DR OMA; QVRPEMI; -.
DR Proteomes; UP000000428; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR CDD; cd03702; IF2_mtIF2_II; 1.
DR Gene3D; 3.40.50.10050; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00100_B; IF_2_B; 1.
DR InterPro; IPR044145; IF2_II.
DR InterPro; IPR006847; IF2_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR000178; TF_IF2_bacterial-like.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR PANTHER; PTHR43381; PTHR43381; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF11987; IF-2; 1.
DR Pfam; PF04760; IF2_N; 2.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50447; SSF50447; 2.
DR SUPFAM; SSF52156; SSF52156; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00487; IF-2; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51722; G_TR_2; 1.
DR PROSITE; PS01176; IF2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; GTP-binding; Initiation factor; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome.
FT CHAIN 1..1046
FT /note="Translation initiation factor IF-2"
FT /id="PRO_0000137260"
FT DOMAIN 539..711
FT /note="tr-type G"
FT REGION 49..450
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 548..555
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 573..577
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 598..601
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 652..655
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 688..690
FT /note="G5"
FT /evidence="ECO:0000250"
FT COMPBIAS 75..91
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 105..137
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 178..193
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 284..301
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 548..555
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT BINDING 598..602
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
FT BINDING 652..655
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00100"
SQ SEQUENCE 1046 AA; 106883 MW; 62B12E2DFA3AA595 CRC64;
MAKVRVYELA KEFGVESKVV MAKLQELGEF VRSASSTIEA PVVRKLTDAL QQGNGGKAAP
RKAAPAKPGA PSPAQAARPA APRPPAPKPA AAERPAAAER PAAAPAAPGP RPGPKPAPRP
APAAPAPAAP EFTAPPSAPA APAAAASGPR PGARPGAPKP GGARPATPGQ GQGGQVRGER
TDRGDRGDRG DRQGAARPGG QAPRPGARPA GPRPGNNPFT SGGSTGMARP QAPRPGGAPR
PGGAGAPGGP RPQGAGGQDR APRPQGGPGG APRPQGGPGG ARPTPGGMPR PQAPRPGGAP
GGNRPNPGMM PQRPAAGPRP GGGGPGGRGP GGGGRPGGPG GGGGRPGGGG FAGRPGGGGG
GFAGRPGGPG GGGGGFAGRP GGPGGGGGGR PGFGGRPGGP GGRGGTQGAF GRPGGPARRG
RKSKRQRRQE YEAMQAPSVG GVMLPRGNGQ SVRLSRGASL TDFAEKIGAN PASLVGVMMN
LGEMVTATQS VSDETLKLLA DEMNFVLEIV SPEEEDRELL ESFDIEFGED EGGEEFLVAR
PPVVTVMGHV DHGKTRLLDT IRKTNVVAGE AGGITQHIGA YQVTTEVNDE ERKITFIDTP
GHEAFTAMRA RGAKSTDIAI LVVAANDGVM PQTIEALNHA KAADVPIVVA VNKIDVEGAD
PTKVRGQLTE FGLVAEEYGG DTMFVDISAK QGLNIESLLE AVVLTADASL DLRANPEQDA
QGIAIESHLD RGRGAVATVL VQRGTLRVGD TMVVGDAYGR VRAMLDDKGE NVEEAGPSTP
VLVLGLTNVP GAGDNFLVVD EDRTARQIAE KRAARERNAN FARRGVRFSL ENLDEALKAG
LVQELNLIIK GDASGSVEAL ESSLLQLDVG EEVDIRILHR GVGAVTESDI NLATGSDAIV
IGFNVRAAGR AAQMAEREGV DVRYYSVIYQ AIEEIEAALK GMLKPEYEEV ELGTAEIREV
FKSSKLGNIA GVLVRSGEVK RNTKARLIRD GKVIAESLNI SGLRRFKDDV TEIREGFEGG
INLGNFNDIK VDDVIATYEM REKPRS
