ID   APEX_BACAN              Reviewed;         743 AA.
AC   Q81QL7; E9RB10; E9RB11; Q6HYT4; Q6KST8;
DT   25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 143.
DE   RecName: Full=Apo-petrobactin exporter {ECO:0000303|PubMed:28900020};
GN   Name=apeX {ECO:0000303|PubMed:28900020};
GN   OrderedLocusNames=GBAA_2407 {ECO:0000312|EMBL:AAT31524.1};
OS   Bacillus anthracis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1392;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames ancestor;
RX   PubMed=18952800; DOI=10.1128/jb.01347-08;
RA   Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA   Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT   "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL   J. Bacteriol. 191:445-446(2009).
RN   [2]
RP   FUNCTION AS A TRANSPORTER, AND DISRUPTION PHENOTYPE.
RC   STRAIN=Sterne;
RX   PubMed=28900020; DOI=10.1128/mbio.01238-17;
RA   Hagan A.K., Tripathi A., Berger D., Sherman D.H., Hanna P.C.;
RT   "Petrobactin is exported from Bacillus anthracis by the RND-type exporter
RT   ApeX.";
RL   MBio 8:E01238-E01238(2017).
CC   -!- FUNCTION: Exports the siderophore petrobactin.
CC       {ECO:0000269|PubMed:28900020}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the gene abrogates export of intact
CC       petrobactin, which accumulates inside the cell. However, growth of the
CC       mutants in iron-depleted medium is not affected, and virulence in mice
CC       is not attenuated. {ECO:0000269|PubMed:28900020}.
CC   -!- SIMILARITY: Belongs to the resistance-nodulation-cell division (RND)
CC       (TC 2.A.6) family. MmpL subfamily. {ECO:0000305}.
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DR   EMBL; AE017334; AAT31524.1; -; Genomic_DNA.
DR   RefSeq; WP_000716545.1; NZ_WXXJ01000001.1.
DR   AlphaFoldDB; Q81QL7; -.
DR   SMR; Q81QL7; -.
DR   IntAct; Q81QL7; 1.
DR   STRING; 260799.BAS2243; -.
DR   DNASU; 1087353; -.
DR   EnsemblBacteria; AAT31524; AAT31524; GBAA_2407.
DR   GeneID; 45022283; -.
DR   KEGG; bar:GBAA_2407; -.
DR   PATRIC; fig|1392.230.peg.2369; -.
DR   HOGENOM; CLU_005108_4_0_9; -.
DR   OMA; IGDRIWW; -.
DR   Proteomes; UP000000594; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR004869; MMPL_dom.
DR   InterPro; IPR000731; SSD.
DR   Pfam; PF03176; MMPL; 2.
DR   PROSITE; PS50156; SSD; 2.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..743
FT                   /note="Apo-petrobactin exporter"
FT                   /id="PRO_0000443812"
FT   TRANSMEM        20..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        199..219
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        223..243
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        258..278
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        303..323
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        337..357
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        406..426
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        561..581
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        584..604
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        613..633
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        672..692
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        694..714
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   743 AA;  81904 MW;  6BF944FE9FF88760 CRC64;
     MKKHPLHMLG RLVAGKNTQW ITLSVWILIT LLLSFTLPQV NSTKEPNPKN LPETAMSQQA
     EALMKKEFPN NAGNPLLVVW YRDGGLQSQD YKLIQDVYKE LKASPLKEQS TLPPFDTIPE
     QVLSKSASKD GTSFVTPVFF NKSAGTDILK ENLDDLRNIV NSKVDEDPFK RKINDAGLHV
     RLSGPVGIQT DAVSLFSQAD VKLLVATVLL VLVLLILLYR SPILAILPLL VVGFAYGIIS
     PTLGFLADHG WIKVDAQAIS IMTVLLFGAG TDYCLFLISR YREYLLEEES KYKALQLAIK
     ASGGAIIMSA LTVVLGLGTL LLAHYGAFHR FAVPFSVAVF IMGIAALTIL PAFLLIFGRT
     AFFPFIPRTT SMNEELARRK KKVVKVKKSK GAFSKKLGDV VVRRPWTIIM LTVFVLGGLA
     SFVPRIQYTY DLLESFPKDM PSREGFTLIS DHFSAGELAP VKVIVDTKGK ELPIKEELEK
     FSFVNTVKDP KEGKENKQIQ MYEVSLAENP YSIEALDQIP KLKNSVEKVF KDAGISNAED
     QLWIGGETAS LYDTKQITER DEAVIIPVMI SIIALLLLVY LRSIVAMIYL IVTVVLSFFS
     ALGAGWLLLH YGMGAPAIQG AIPLYAFVFL VALGEDYNIF MVSEIWKNRK TQNHLDAVKN
     GVIQTGSVIT SAGLILAGTF AVLGTLPIQV LVQFGIVTAI GVLLDTFIVR PLLVPAITVV
     LGRFAFWPGK LSRKSEEVQK VDA