APG12_DROME
ID APG12_DROME Reviewed; 111 AA.
AC Q9VTU1; Q4V6A9;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 3.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Autophagy protein 12-like;
DE Short=APG12-like;
GN Name=Atg12; ORFNames=CG10861;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION.
RX PubMed=15296714; DOI=10.1016/j.devcel.2004.07.009;
RA Scott R.C., Schuldiner O., Neufeld T.P.;
RT "Role and regulation of starvation-induced autophagy in the Drosophila fat
RT body.";
RL Dev. Cell 7:167-178(2004).
CC -!- FUNCTION: Required for autophagy. {ECO:0000269|PubMed:15296714}.
CC -!- SUBUNIT: Conjugated to autophagy protein 5-like. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- PTM: Conjugation of the G-112 to the K-132 of Autophagy protein 5-like
CC is a covalent modification that is essential for autophagy.
CC {ECO:0000250}.
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DR EMBL; AE014296; AAF49955.4; -; Genomic_DNA.
DR EMBL; BT022374; AAY54790.1; -; mRNA.
DR EMBL; BT022397; AAY54813.1; -; mRNA.
DR RefSeq; NP_648551.3; NM_140294.4.
DR AlphaFoldDB; Q9VTU1; -.
DR SMR; Q9VTU1; -.
DR BioGRID; 64738; 9.
DR IntAct; Q9VTU1; 1.
DR STRING; 7227.FBpp0289288; -.
DR PaxDb; Q9VTU1; -.
DR PRIDE; Q9VTU1; -.
DR DNASU; 39383; -.
DR EnsemblMetazoa; FBtr0300011; FBpp0289288; FBgn0036255.
DR GeneID; 39383; -.
DR KEGG; dme:Dmel_CG10861; -.
DR CTD; 9140; -.
DR FlyBase; FBgn0036255; Atg12.
DR VEuPathDB; VectorBase:FBgn0036255; -.
DR eggNOG; KOG3439; Eukaryota.
DR GeneTree; ENSGT00390000016654; -.
DR HOGENOM; CLU_106795_3_1_1; -.
DR InParanoid; Q9VTU1; -.
DR OMA; ADLPMNM; -.
DR OrthoDB; 1525971at2759; -.
DR PhylomeDB; Q9VTU1; -.
DR Reactome; R-DME-1632852; Macroautophagy.
DR Reactome; R-DME-5205685; PINK1-PRKN Mediated Mitophagy.
DR BioGRID-ORCS; 39383; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 39383; -.
DR PRO; PR:Q9VTU1; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036255; Expressed in saliva-secreting gland and 29 other tissues.
DR Genevisible; Q9VTU1; DM.
DR GO; GO:0034274; C:Atg12-Atg5-Atg16 complex; ISS:FlyBase.
DR GO; GO:0034045; C:phagophore assembly site membrane; IBA:GO_Central.
DR GO; GO:0031386; F:protein tag; ISS:FlyBase.
DR GO; GO:0000045; P:autophagosome assembly; ISS:FlyBase.
DR GO; GO:0006914; P:autophagy; IMP:FlyBase.
DR GO; GO:0000422; P:autophagy of mitochondrion; IBA:GO_Central.
DR GO; GO:0044804; P:autophagy of nucleus; IBA:GO_Central.
DR GO; GO:0006501; P:C-terminal protein lipidation; IBA:GO_Central.
DR GO; GO:0009267; P:cellular response to starvation; IMP:FlyBase.
DR GO; GO:0061723; P:glycophagy; IMP:FlyBase.
DR GO; GO:0035096; P:larval midgut cell programmed cell death; IMP:FlyBase.
DR GO; GO:0016236; P:macroautophagy; IMP:FlyBase.
DR InterPro; IPR007242; Atg12.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR13385; PTHR13385; 1.
DR Pfam; PF04110; APG12; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
PE 3: Inferred from homology;
KW Autophagy; Cytoplasm; Isopeptide bond; Reference proteome;
KW Ubl conjugation pathway.
FT CHAIN 1..111
FT /note="Autophagy protein 12-like"
FT /id="PRO_0000064632"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..21
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 111
FT /note="Glycyl lysine isopeptide (Gly-Lys) (interchain with
FT K-132 in APG5)"
FT /evidence="ECO:0000250"
SQ SEQUENCE 111 AA; 12348 MW; 6FA2EBDA01BC1377 CRC64;
MAETPESQAA LSTSSSTPAD KDGSKICILL NATGNVPIIK KRTWTVDPNK TVGWIQTFIH
KFLKLDASEQ IFLYVNQTFA PAPDQIIKNL YECHGTNGKL VLYYCKNQAW G
