ID   IF3_MYXXA               Reviewed;         247 AA.
AC   P48516;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Translation initiation factor IF-3 {ECO:0000255|HAMAP-Rule:MF_00080};
GN   Name=infC {ECO:0000255|HAMAP-Rule:MF_00080}; Synonyms=dsg;
OS   Myxococcus xanthus.
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Myxococcaceae; Myxococcus.
OX   NCBI_TaxID=34;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-7.
RC   STRAIN=DK101;
RX   PubMed=8113184; DOI=10.1128/jb.176.5.1427-1433.1994;
RA   Cheng Y.L., Kalman L.V., Kaiser D.;
RT   "The dsg gene of Myxococcus xanthus encodes a protein similar to
RT   translation initiation factor IF3.";
RL   J. Bacteriol. 176:1427-1433(1994).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=8113185; DOI=10.1128/jb.176.5.1434-1442.1994;
RA   Kalman L.V., Cheng Y.L., Kaiser D.;
RT   "The Myxococcus xanthus dsg gene product performs functions of translation
RT   initiation factor IF3 in vivo.";
RL   J. Bacteriol. 176:1434-1442(1994).
RN   [3]
RP   CHARACTERIZATION.
RC   STRAIN=DK101;
RX   PubMed=2544552; DOI=10.1128/jb.171.7.3719-3726.1989;
RA   Cheng Y.L., Kaiser D.;
RT   "dsg, a gene required for cell-cell interaction early in Myxococcus
RT   development.";
RL   J. Bacteriol. 171:3719-3726(1989).
CC   -!- FUNCTION: IF-3 binds to the 30S ribosomal subunit and shifts the
CC       equilibrum between 70S ribosomes and their 50S and 30S subunits in
CC       favor of the free subunits, thus enhancing the availability of 30S
CC       subunits on which protein synthesis initiation begins.
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00080}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00080}.
CC   -!- DOMAIN: The 66 residues C-terminal tail is not present in other
CC       species.
CC   -!- SIMILARITY: Belongs to the IF-3 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00080}.
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DR   EMBL; U04438; AAC13748.1; -; Unassigned_DNA.
DR   PIR; A53379; A53379.
DR   RefSeq; WP_011550712.1; NZ_FNOH01000002.1.
DR   AlphaFoldDB; P48516; -.
DR   SMR; P48516; -.
DR   GeneID; 41358060; -.
DR   OMA; RFLAITC; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.10.20.80; -; 1.
DR   Gene3D; 3.30.110.10; -; 1.
DR   HAMAP; MF_00080; IF_3; 1.
DR   InterPro; IPR036788; T_IF-3_C_sf.
DR   InterPro; IPR036787; T_IF-3_N_sf.
DR   InterPro; IPR019813; Translation_initiation_fac3_CS.
DR   InterPro; IPR001288; Translation_initiation_fac_3.
DR   InterPro; IPR019815; Translation_initiation_fac_3_C.
DR   InterPro; IPR019814; Translation_initiation_fac_3_N.
DR   PANTHER; PTHR10938; PTHR10938; 1.
DR   Pfam; PF00707; IF3_C; 1.
DR   Pfam; PF05198; IF3_N; 1.
DR   SUPFAM; SSF54364; SSF54364; 1.
DR   SUPFAM; SSF55200; SSF55200; 1.
DR   TIGRFAMs; TIGR00168; infC; 1.
DR   PROSITE; PS00938; IF3; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Initiation factor;
KW   Protein biosynthesis.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8113184"
FT   CHAIN           2..247
FT                   /note="Translation initiation factor IF-3"
FT                   /id="PRO_0000177547"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          182..247
FT                   /note="Needed for vegetative and developmental functions,
FT                   but not for viability"
FT   REGION          188..247
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        220..234
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         134
FT                   /note="G -> E (in strain: DK429 and DK439; causes defects
FT                   in fruiting body development and in sporulation)"
SQ   SEQUENCE   247 AA;  27145 MW;  6052DAC6407FAEA7 CRC64;
     MIREQRSSRG GSRDQRTNRR IRAREVRVVG SDGSQLGVMP LEAALDRART EGLDLVEISP
     MASPPVCKIM DYGKFKYEEK KKASEAKRAQ VTVLLKEVKL RPKTEEHDYE FKVRNTRRFI
     EDGNKAKVVI QFRGREITHR EQGTAILDDV AKDLKDVAVV EQMPRMEGRL MFMILAPTPK
     VAQKARELVR QAATAAKRPP PPGAPGAGKS AAGASSGAEE KAEETAEEKK EAQAAPAAAE
     AQSPTAS