APG3_ARATH
ID APG3_ARATH Reviewed; 422 AA.
AC Q8RX79; Q9LYD0;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Peptide chain release factor APG3, chloroplastic {ECO:0000305};
DE AltName: Full=Chloroplast ribosome release factor 1 {ECO:0000303|PubMed:17450416};
DE Short=AtcpRF1 {ECO:0000303|PubMed:17450416};
DE AltName: Full=Protein ALBINO AND PALE GREEN 3 {ECO:0000303|PubMed:17450416};
DE Flags: Precursor;
GN Name=APG3 {ECO:0000303|PubMed:17450416};
GN Synonyms=RF1 {ECO:0000303|PubMed:17450416};
GN OrderedLocusNames=At3g62910 {ECO:0000312|Araport:AT3G62910};
GN ORFNames=T20O10.10 {ECO:0000312|EMBL:BAC82693.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Motohashi R., Ito T., Ito K., Nagata N., Yoshida S., Shinozaki K.;
RT "Functional analysis of the chloroplast Release Factor 1 using a Ds-tagged
RT Arabidopsis albino mutant.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY LIGHT, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=17450416; DOI=10.1007/s11103-007-9166-7;
RA Motohashi R., Yamazaki T., Myouga F., Ito T., Ito K., Satou M.,
RA Kobayashi M., Nagata N., Yoshida S., Nagashima A., Tanaka K., Takahashi S.,
RA Shinozaki K.;
RT "Chloroplast ribosome release factor 1 (AtcpRF1) is essential for
RT chloroplast development.";
RL Plant Mol. Biol. 64:481-497(2007).
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=24793022; DOI=10.1007/s11103-014-0194-9;
RA Satou M., Enoki H., Oikawa A., Ohta D., Saito K., Hachiya T.,
RA Sakakibara H., Kusano M., Fukushima A., Saito K., Kobayashi M., Nagata N.,
RA Myouga F., Shinozaki K., Motohashi R.;
RT "Integrated analysis of transcriptome and metabolome of Arabidopsis albino
RT or pale green mutants with disrupted nuclear-encoded chloroplast
RT proteins.";
RL Plant Mol. Biol. 85:411-428(2014).
CC -!- FUNCTION: May direct the termination of translation in response to the
CC peptide chain termination codon UAG. Required for normal chloroplast
CC development and plays essential roles in the termination of translation
CC in plastids. {ECO:0000269|PubMed:17450416}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:17450416}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves and stems.
CC {ECO:0000269|PubMed:17450416}.
CC -!- INDUCTION: By light. {ECO:0000269|PubMed:17450416}.
CC -!- DISRUPTION PHENOTYPE: Albino and pale green (apg) phenotype and early
CC seedling growth arrest. {ECO:0000269|PubMed:17450416,
CC ECO:0000269|PubMed:24793022}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB87736.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB109893; BAC82693.1; -; mRNA.
DR EMBL; AL163816; CAB87736.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE80410.1; -; Genomic_DNA.
DR EMBL; AY090253; AAL90914.1; -; mRNA.
DR EMBL; AY125535; AAM78045.1; -; mRNA.
DR PIR; T48080; T48080.
DR RefSeq; NP_191850.2; NM_116156.5.
DR AlphaFoldDB; Q8RX79; -.
DR SMR; Q8RX79; -.
DR STRING; 3702.AT3G62910.1; -.
DR iPTMnet; Q8RX79; -.
DR PaxDb; Q8RX79; -.
DR PRIDE; Q8RX79; -.
DR ProteomicsDB; 246948; -.
DR EnsemblPlants; AT3G62910.1; AT3G62910.1; AT3G62910.
DR GeneID; 825466; -.
DR Gramene; AT3G62910.1; AT3G62910.1; AT3G62910.
DR KEGG; ath:AT3G62910; -.
DR Araport; AT3G62910; -.
DR TAIR; locus:2099137; AT3G62910.
DR eggNOG; KOG2726; Eukaryota.
DR HOGENOM; CLU_036856_0_1_1; -.
DR InParanoid; Q8RX79; -.
DR OMA; ISDHRVG; -.
DR OrthoDB; 1313171at2759; -.
DR PhylomeDB; Q8RX79; -.
DR PRO; PR:Q8RX79; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8RX79; baseline and differential.
DR Genevisible; Q8RX79; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0003747; F:translation release factor activity; IGI:TAIR.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:InterPro.
DR GO; GO:0009658; P:chloroplast organization; IMP:TAIR.
DR GO; GO:0032544; P:plastid translation; IGI:TAIR.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0010027; P:thylakoid membrane organization; IMP:TAIR.
DR GO; GO:0006415; P:translational termination; IGI:TAIR.
DR HAMAP; MF_00093; Rel_fac_1; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004373; RF-1.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR TIGRFAMs; TIGR00019; prfA; 1.
DR PROSITE; PS00745; RF_PROK_I; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Growth regulation; Plastid; Protein biosynthesis;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..50
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 51..422
FT /note="Peptide chain release factor APG3, chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000430967"
SQ SEQUENCE 422 AA; 47529 MW; 7896B320403FCCA1 CRC64;
MNSSMTTSPT ARAFLHLHSS PFTLRSRKSP SGVCLVSWPP SRSRRTRRLV CMAEPYLIRK
MESVEKTWKE LSVKLADPDV VSNQSEYQKL AQSMSELDEV VTVFRRFKDC EKQLLESKVL
AKEAGDDEDM AEMIGSEINS LTKEIEELEK QLKVLLLPSD PLDARNILLE VRAGTGGDEA
AIWTGDLVRM YQRYSERSSW KFSMVSCSEA EHGGYKTCVM EIKGNRVYSK LKYESGVHRV
QRVPQTETQG RVHTSTATVA IMPEADEVEV VIDPKDIELT SARSGGAGGQ NVNKVETAID
LFHKPSGIRI FCTEERTQIR NKARAFQLLR AKLYEIKVRE QQEKIRNERK SQVGTGARSE
KIRTYNYKDS RVTDHRLKMN FALTTFLDGA LEDAVQACAA LEQKELMEEL SESVAASSAT
SG
