ID   IF413_TOBAC             Reviewed;         355 AA.
AC   Q40466;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Eukaryotic initiation factor 4A-13;
DE            Short=eIF-4A-13;
DE            EC=3.6.4.13;
DE   AltName: Full=ATP-dependent RNA helicase eIF4A-13;
DE   Flags: Fragment;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. SR1;
RX   PubMed=7858215; DOI=10.1007/bf00019489;
RA   Owttrim G.W., Mandel T., Trachsel H., Thomas A.A., Kuhlemeier C.;
RT   "Characterization of the tobacco eIF-4A gene family.";
RL   Plant Mol. Biol. 26:1747-1757(1994).
CC   -!- FUNCTION: ATP-dependent RNA helicase which is a subunit of the eIF4F
CC       complex involved in cap recognition and is required for mRNA binding to
CC       ribosome. In the current model of translation initiation, eIF4A unwinds
CC       RNA secondary structures in the 5'-UTR of mRNAs which is necessary to
CC       allow efficient binding of the small ribosomal subunit, and subsequent
CC       scanning for the initiator codon (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: eIF4F is a multi-subunit complex, the composition of which
CC       varies with external and internal environmental conditions. It is
CC       composed of at least EIF4A, EIF4E and EIF4G (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. eIF4A subfamily.
CC       {ECO:0000305}.
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DR   EMBL; X79140; CAA55741.1; -; mRNA.
DR   PIR; S52022; S52022.
DR   AlphaFoldDB; Q40466; -.
DR   SMR; Q40466; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR   GO; GO:0002183; P:cytoplasmic translational initiation; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Helicase; Hydrolase; Initiation factor; Nucleotide-binding;
KW   Protein biosynthesis; Reference proteome; RNA-binding.
FT   CHAIN           1..>355
FT                   /note="Eukaryotic initiation factor 4A-13"
FT                   /id="PRO_0000054959"
FT   DOMAIN          71..241
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          252..>355
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   MOTIF           40..68
FT                   /note="Q motif"
FT   MOTIF           189..192
FT                   /note="DEAD box"
FT   BINDING         84..91
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   NON_TER         355
SQ   SEQUENCE   355 AA;  40215 MW;  8E5429C1C5D01E72 CRC64;
     MAGLAPEGSQ FDARQYDAKM TELLGTEQEE FFTSYDEVYD SFDAMGLQEN LLRGIYAYGF
     EKPSAIQQRG IVPFCKGLDV IQQAQSGTGK TATFCSGVLQ QLDYSLVECQ ALVLAPTREL
     AQQIEKVMRA LGDYLGVKVH ACVGGTSVRE DQRILQSGVH VVVGTPGRVF DMLRRQSLRP
     DHIKMFVLDE ADEMLSRGFK DQIYDIFQLL PPKIQVGVFS ATMPPEALEI TRKFMNQPVR
     ILVKRDDVTL EGIKQFYVNV DKEEWKLETL CDLYETLAIT QSVIFVNTRR KVDWLTDKMR
     SRDHTVSATH GDMDQNTRDI IMREFRSGSS RVLITTDLLA RGIDVQQVSL VINYD