ID   IF4A1_ORYSJ             Reviewed;         414 AA.
AC   P35683; B7EPR0; Q0D9S8; Q54AC9; Q5Z847;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 2.
DT   03-AUG-2022, entry version 140.
DE   RecName: Full=Eukaryotic initiation factor 4A-1;
DE            Short=eIF-4A-1;
DE            EC=3.6.4.13;
DE   AltName: Full=ATP-dependent RNA helicase eIF4A-1;
DE   AltName: Full=DEAD-box ATP-dependent RNA helicase 4;
GN   OrderedLocusNames=Os06g0701100, LOC_Os06g48750;
GN   ORFNames=OsJ_22532, P0468G03.33, P0596H10.2;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Yamahoushi;
RX   PubMed=8373808; DOI=10.1016/0167-4781(93)90201-n;
RA   Nishi R., Kidou S., Uchimiya H., Kato A.;
RT   "Isolation and characterization of a rice cDNA which encodes the eukaryotic
RT   initiation factor 4A.";
RL   Biochim. Biophys. Acta 1174:293-294(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11913775; DOI=10.3109/10425170109084453;
RA   Kato A., Fujita S., Komeda Y.;
RT   "Identification and characterization of two genes encoding the eukaryotic
RT   initiation factor 4A in rice.";
RL   DNA Seq. 12:295-303(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: ATP-dependent RNA helicase which is a subunit of the eIF4F
CC       complex involved in cap recognition and is required for mRNA binding to
CC       ribosome. In the current model of translation initiation, eIF4A unwinds
CC       RNA secondary structures in the 5'-UTR of mRNAs which is necessary to
CC       allow efficient binding of the small ribosomal subunit, and subsequent
CC       scanning for the initiator codon (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: eIF4F is a multi-subunit complex, the composition of which
CC       varies with external and internal environmental conditions. It is
CC       composed of at least EIF4A, EIF4E and EIF4G (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. eIF4A subfamily.
CC       {ECO:0000305}.
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DR   EMBL; D12627; BAA02152.1; -; mRNA.
DR   EMBL; AB046416; BAB21260.1; -; Genomic_DNA.
DR   EMBL; AP003726; BAD53769.1; -; Genomic_DNA.
DR   EMBL; AP004278; BAD54014.1; -; Genomic_DNA.
DR   EMBL; AP008212; BAF20395.1; -; Genomic_DNA.
DR   EMBL; AP014962; BAS99344.1; -; Genomic_DNA.
DR   EMBL; CM000143; EAZ38180.1; -; Genomic_DNA.
DR   EMBL; AK099927; BAG94357.1; -; mRNA.
DR   EMBL; AK105454; BAG97257.1; -; mRNA.
DR   EMBL; AK119234; BAG99594.1; -; mRNA.
DR   PIR; S38358; S38358.
DR   RefSeq; XP_015643172.1; XM_015787686.1.
DR   AlphaFoldDB; P35683; -.
DR   SMR; P35683; -.
DR   STRING; 4530.OS06T0701100-02; -.
DR   PaxDb; P35683; -.
DR   PRIDE; P35683; -.
DR   EnsemblPlants; Os06t0701100-02; Os06t0701100-02; Os06g0701100.
DR   GeneID; 4341966; -.
DR   Gramene; Os06t0701100-02; Os06t0701100-02; Os06g0701100.
DR   KEGG; osa:4341966; -.
DR   eggNOG; KOG0327; Eukaryota.
DR   InParanoid; P35683; -.
DR   OMA; EHKDGQR; -.
DR   OrthoDB; 726081at2759; -.
DR   Proteomes; UP000000763; Chromosome 6.
DR   Proteomes; UP000007752; Chromosome 6.
DR   Proteomes; UP000059680; Chromosome 6.
DR   ExpressionAtlas; P35683; baseline and differential.
DR   Genevisible; P35683; OS.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR   GO; GO:0002183; P:cytoplasmic translational initiation; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Helicase; Hydrolase; Initiation factor; Nucleotide-binding;
KW   Protein biosynthesis; Reference proteome; RNA-binding.
FT   CHAIN           1..414
FT                   /note="Eukaryotic initiation factor 4A-1"
FT                   /id="PRO_0000054963"
FT   DOMAIN          72..242
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          253..414
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   MOTIF           41..69
FT                   /note="Q motif"
FT   MOTIF           190..193
FT                   /note="DEAD box"
FT   BINDING         85..92
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   CONFLICT        291
FT                   /note="Missing (in Ref. 1; BAA02152 and 2; BAB21260)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   414 AA;  47088 MW;  3606C5259BBF0302 CRC64;
     MAGMAPEGSQ FDAKHYDSKM QELLNQGETE EFFTSYDEVH ESFDDMGLQE NLLRGIYAYG
     FEKPSAIQQR GIVPFCKGLD VIQQAQSGTG KTATFCSGIL QQLDYAVVEC QALVLAPTRE
     LAQQIEKVMR ALGDYLGVKV HACVGGTSVR EDQRILASGV HVVVGTPGRV FDMLRRQSLR
     PDYIKMFVLD EADEMLSRGF KDQIYDIFQL LPSKIQVGVF SATMPPEALE ITRKFMNKPV
     RILVKRDELT LEGIKQFYVN VEKEEWKLDT LCDLYETLAI TQSVIFVNTR RKVDWLTDKM
     RGRDHTVSAT HGDMDQNTRD IIMREFRSGS SRVLITTDLL ARGIDVQQVS LVINYDLPTQ
     PENYLHRIGR SGRFGRKGVA INFVTRDDER MLFDIQRFYN VVIEELPANV ADLL