IF4A3_ORYSJ
ID IF4A3_ORYSJ Reviewed; 414 AA.
AC Q6Z2Z4; B7EMF9; Q9AR32;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Eukaryotic initiation factor 4A-3;
DE Short=eIF-4A-3;
DE EC=3.6.4.13;
DE AltName: Full=ATP-dependent RNA helicase eIF4A-3;
DE AltName: Full=DEAD-box ATP-dependent RNA helicase 23;
DE AltName: Full=eIF4A-2;
GN OrderedLocusNames=Os02g0146600, LOC_Os02g05330;
GN ORFNames=OJ1008_C03.10, OsJ_05367 {ECO:0000312|EMBL:EEE56296.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX PubMed=11913775; DOI=10.3109/10425170109084453;
RA Kato A., Fujita S., Komeda Y.;
RT "Identification and characterization of two genes encoding the eukaryotic
RT initiation factor 4A in rice.";
RL DNA Seq. 12:295-303(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Ilpoombyeo; TISSUE=Immature seed;
RA Yoon U.H.;
RT "Structural and expression analysis of immature seed genes in Oryza sativa
RT L.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [8]
RP INTERACTION WITH DRM2, AND SUBCELLULAR LOCATION.
RX PubMed=23732981; DOI=10.1016/j.jmb.2013.05.021;
RA Dangwal M., Malik G., Kapoor S., Kapoor M.;
RT "De novo methyltransferase, OsDRM2, interacts with the ATP-dependent RNA
RT helicase, OseIF4A, in rice.";
RL J. Mol. Biol. 425:2853-2866(2013).
CC -!- FUNCTION: ATP-dependent RNA helicase which is a subunit of the eIF4F
CC complex involved in cap recognition and is required for mRNA binding to
CC ribosome. In the current model of translation initiation, eIF4A unwinds
CC RNA secondary structures in the 5'-UTR of mRNAs which is necessary to
CC allow efficient binding of the small ribosomal subunit, and subsequent
CC scanning for the initiator codon (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBUNIT: eIF4F is a multi-subunit complex, the composition of which
CC varies with external and internal environmental conditions. It is
CC composed of at least EIF4A, EIF4E and EIF4G (By similarity). Interacts
CC with DRM2 (via UBA domains) (PubMed:23732981). {ECO:0000250,
CC ECO:0000269|PubMed:23732981}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus
CC {ECO:0000269|PubMed:23732981}. Note=Localizes to the nucleus when
CC interacting with DRM2. {ECO:0000269|PubMed:23732981}.
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. eIF4A subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB21258.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAB21259.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB046414; BAB21258.1; ALT_FRAME; mRNA.
DR EMBL; AB046415; BAB21259.1; ALT_FRAME; Genomic_DNA.
DR EMBL; KC140119; AGT42311.1; -; mRNA.
DR EMBL; AP005288; BAD13081.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF07792.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS76965.1; -; Genomic_DNA.
DR EMBL; CM000139; EEE56296.1; -; Genomic_DNA.
DR EMBL; AK073620; BAG93556.1; -; mRNA.
DR RefSeq; XP_015626460.1; XM_015770974.1.
DR AlphaFoldDB; Q6Z2Z4; -.
DR SMR; Q6Z2Z4; -.
DR STRING; 4530.OS02T0146600-01; -.
DR PaxDb; Q6Z2Z4; -.
DR PRIDE; Q6Z2Z4; -.
DR EnsemblPlants; Os02t0146600-01; Os02t0146600-01; Os02g0146600.
DR GeneID; 4328286; -.
DR Gramene; Os02t0146600-01; Os02t0146600-01; Os02g0146600.
DR KEGG; osa:4328286; -.
DR eggNOG; KOG0327; Eukaryota.
DR HOGENOM; CLU_003041_1_0_1; -.
DR InParanoid; Q6Z2Z4; -.
DR OMA; HLGDYMN; -.
DR OrthoDB; 726081at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6Z2Z4; OS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR GO; GO:0002183; P:cytoplasmic translational initiation; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cytoplasm; Helicase; Hydrolase; Initiation factor;
KW Nucleotide-binding; Nucleus; Protein biosynthesis; Reference proteome;
KW RNA-binding.
FT CHAIN 1..414
FT /note="Eukaryotic initiation factor 4A-3"
FT /id="PRO_0000282441"
FT DOMAIN 72..242
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 253..414
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 41..69
FT /note="Q motif"
FT MOTIF 190..193
FT /note="DEAD box"
FT BINDING 85..92
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT CONFLICT 38..39
FT /note="EV -> DL (in Ref. 1; BAB21258/BAB21259)"
FT /evidence="ECO:0000305"
FT CONFLICT 69
FT /note="Q -> K (in Ref. 1; BAB21258/BAB21259)"
FT /evidence="ECO:0000305"
FT CONFLICT 152
FT /note="D -> Q (in Ref. 1; BAB21258/BAB21259)"
FT /evidence="ECO:0000305"
FT CONFLICT 228
FT /note="A -> G (in Ref. 1; BAB21258/BAB21259)"
FT /evidence="ECO:0000305"
FT CONFLICT 233..234
FT /note="RK -> LR (in Ref. 1; BAB21258/BAB21259)"
FT /evidence="ECO:0000305"
FT CONFLICT 237
FT /note="N -> D (in Ref. 1; BAB21258/BAB21259)"
FT /evidence="ECO:0000305"
FT CONFLICT 251
FT /note="L -> R (in Ref. 1; BAB21258/BAB21259)"
FT /evidence="ECO:0000305"
FT CONFLICT 276..277
FT /note="ET -> DS (in Ref. 1; BAB21258/BAB21259)"
FT /evidence="ECO:0000305"
FT CONFLICT 290..292
FT /note="RRK -> LRM (in Ref. 1; BAB21258/BAB21259)"
FT /evidence="ECO:0000305"
FT CONFLICT 317
FT /note="N -> I (in Ref. 1; BAB21258/BAB21259)"
FT /evidence="ECO:0000305"
FT CONFLICT 341
FT /note="A -> D (in Ref. 1; BAB21258/BAB21259)"
FT /evidence="ECO:0000305"
FT CONFLICT 358
FT /note="P -> S (in Ref. 1; BAB21258/BAB21259)"
FT /evidence="ECO:0000305"
FT CONFLICT 361
FT /note="P -> T (in Ref. 1; BAB21258/BAB21259)"
FT /evidence="ECO:0000305"
FT CONFLICT 382
FT /note="N -> I (in Ref. 1; BAB21258/BAB21259)"
FT /evidence="ECO:0000305"
FT CONFLICT 401
FT /note="V -> G (in Ref. 1; BAB21258/BAB21259)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 414 AA; 47138 MW; B440EF6727D791DD CRC64;
MAGMAPEGSQ FDAKHYDSKM QELLHQGDNE EFFTSYDEVF ESFDDMGLQE NLLRGIYAYG
FEKPSAIQQR GIVPFCKGLD VIQQAQSGTG KTATFCSGIL QQLDYGLVEC QSLVLAPTRE
LAQQIEKVMR ALGDYLGVKV HACVGGTSVR EDQRILASGV HVVVGTPGRV FDMLRRQSLR
PDHIKMFVLD EADEMLSRGF KDQIYDIFQL LPPKIQVGVF SATMPPEALE ITRKFMNKPV
RILVKRDELT LEGIKQFYVN VEKEDWKLDT LCDLYETLAI TQSVIFVNTR RKVDWLTDKM
RSRDHTVSAT HGDMDQNTRD IIMREFRSGS SRVLITTDLL ARGIDVQQVS LVINYDLPTQ
PENYLHRIGR SGRFGRKGVA INFVTRDDER MLFDIQRFYN VTIEELPANV ADLL
