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IF4A3_XENTR
ID   IF4A3_XENTR             Reviewed;         415 AA.
AC   B7ZTW1; A9ULA1;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Eukaryotic initiation factor 4A-III;
DE            Short=eIF-4A-III;
DE            Short=eIF4A-III;
DE            EC=3.6.4.13 {ECO:0000250|UniProtKB:P38919};
DE   AltName: Full=ATP-dependent RNA helicase DDX48;
DE   AltName: Full=ATP-dependent RNA helicase eIF4A-3;
DE   AltName: Full=DEAD box protein 48;
DE   AltName: Full=Eukaryotic translation initiation factor 4A isoform 3;
GN   Name=eif4a3; Synonyms=ddx48;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: ATP-dependent RNA helicase. Involved in pre-mRNA splicing as
CC       component of the spliceosome. Core component of the splicing-dependent
CC       multiprotein exon junction complex (EJC) deposited at splice junctions
CC       on mRNAs. The EJC is a dynamic structure consisting of core proteins
CC       and several peripheral nuclear and cytoplasmic associated factors that
CC       join the complex only transiently either during EJC assembly or during
CC       subsequent mRNA metabolism. The EJC marks the position of the exon-exon
CC       junction in the mature mRNA for the gene expression machinery and the
CC       core components remain bound to spliced mRNAs throughout all stages of
CC       mRNA metabolism thereby influencing downstream processes including
CC       nuclear mRNA export, subcellular mRNA localization, translation
CC       efficiency and nonsense-mediated mRNA decay (NMD). Binds spliced mRNA
CC       in sequence-independent manner, 20-24 nucleotides upstream of mRNA
CC       exon-exon junctions (By similarity). Involved in craniofacial
CC       development (By similarity). {ECO:0000250|UniProtKB:P38919}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC         Evidence={ECO:0000250|UniProtKB:P38919};
CC   -!- SUBUNIT: Identified in the spliceosome C complex. Part of the mRNA
CC       splicing-dependent exon junction complex (EJC) complex; the core
CC       complex contains casc3, eif4a3, magoh and rbm8a.
CC       {ECO:0000250|UniProtKB:P38919}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q3B8Q2}. Nucleus
CC       speckle {ECO:0000250|UniProtKB:P38919}. Cytoplasm
CC       {ECO:0000250|UniProtKB:Q3B8Q2}. Note=Nucleocytoplasmic shuttling
CC       protein. Travels to the cytoplasm as part of the exon junction complex
CC       (EJC) bound to mRNA. {ECO:0000250|UniProtKB:Q3B8Q2}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. eIF4A subfamily.
CC       {ECO:0000305}.
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DR   EMBL; BC157177; AAI57178.1; -; mRNA.
DR   EMBL; BC171014; AAI71014.1; -; mRNA.
DR   RefSeq; NP_001107349.1; NM_001113877.1.
DR   AlphaFoldDB; B7ZTW1; -.
DR   SMR; B7ZTW1; -.
DR   STRING; 8364.ENSXETP00000030049; -.
DR   PaxDb; B7ZTW1; -.
DR   Ensembl; ENSXETT00000030049; ENSXETP00000030049; ENSXETG00000013720.
DR   GeneID; 100135173; -.
DR   KEGG; xtr:100135173; -.
DR   CTD; 100135173; -.
DR   Xenbase; XB-GENE-5773453; eif4a3.1.
DR   eggNOG; KOG0328; Eukaryota.
DR   HOGENOM; CLU_003041_1_0_1; -.
DR   InParanoid; B7ZTW1; -.
DR   OMA; FTHRNGR; -.
DR   OrthoDB; 726081at2759; -.
DR   TreeFam; TF300466; -.
DR   Reactome; R-XTR-1169408; ISG15 antiviral mechanism.
DR   Reactome; R-XTR-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR   Reactome; R-XTR-429947; Deadenylation of mRNA.
DR   Reactome; R-XTR-72163; mRNA Splicing - Major Pathway.
DR   Reactome; R-XTR-72187; mRNA 3'-end processing.
DR   Reactome; R-XTR-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
DR   Proteomes; UP000008143; Chromosome 5.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000013720; Expressed in egg cell and 16 other tissues.
DR   ExpressionAtlas; B7ZTW1; baseline.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR   GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR   GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR   GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
DR   GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Helicase; Hydrolase; mRNA processing;
KW   mRNA splicing; mRNA transport; Nonsense-mediated mRNA decay;
KW   Nucleotide-binding; Nucleus; Reference proteome; RNA-binding; Spliceosome;
KW   Translation regulation; Transport.
FT   CHAIN           1..415
FT                   /note="Eukaryotic initiation factor 4A-III"
FT                   /id="PRO_0000378562"
FT   DOMAIN          73..243
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          254..415
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   MOTIF           42..70
FT                   /note="Q motif"
FT   MOTIF           191..194
FT                   /note="DEAD box"
FT                   /evidence="ECO:0000305"
FT   BINDING         64
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P38919"
FT   BINDING         69
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P38919"
FT   BINDING         86..93
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   BINDING         89..94
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P38919"
FT   BINDING         346
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P38919"
FT   BINDING         371..375
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P38919"
FT   CONFLICT        4
FT                   /note="A -> V (in Ref. 1; AAI57178)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   415 AA;  47155 MW;  28B166505E87383D CRC64;
     MAAAAVTAVA GVTSGSSRKR LLREEDMTKV EFETSEEVDV TPTFDTMGLR EDLLRGIYAY
     GFEKPSAIQQ RAIKQIIKGR DVIAQSQSGT GKTATFCVSV LQCLDIQVRE TQALILAPTR
     ELAGQIQKVL LALGDYMNVQ CHACIGGTNV GEDIRKLDYG QHVVAGTPGR VFDMIRRRSL
     RTRAIKMLVL DEADEMLNKG FKEQIYDVYR YLPPATQVCL ISATLPHEIL EMTNKFMTDP
     IRILVKRDEL TLEGIKQFFV AVEREEWKFD TLCDLYDTLT ITQAVIFCNT KRKVDWLTEK
     MREANFTVSS MHGDMPQKER ESIMKEFRSG ASRVLISTDV WARGLDVPQV SLIINYDLPN
     NRELYIHRIG RSGRYGRKGV AINFVKNDDI RILRDIEQYY STQIDEMPMN VADLI
 
 
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