IF4A6_TOBAC
ID IF4A6_TOBAC Reviewed; 253 AA.
AC Q40469;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Eukaryotic initiation factor 4A-6;
DE Short=eIF-4A-6;
DE EC=3.6.4.13;
DE AltName: Full=ATP-dependent RNA helicase eIF4A-6;
DE Flags: Fragment;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. SR1;
RX PubMed=7858215; DOI=10.1007/bf00019489;
RA Owttrim G.W., Mandel T., Trachsel H., Thomas A.A., Kuhlemeier C.;
RT "Characterization of the tobacco eIF-4A gene family.";
RL Plant Mol. Biol. 26:1747-1757(1994).
CC -!- FUNCTION: ATP-dependent RNA helicase which is a subunit of the eIF4F
CC complex involved in cap recognition and is required for mRNA binding to
CC ribosome. In the current model of translation initiation, eIF4A unwinds
CC RNA secondary structures in the 5'-UTR of mRNAs which is necessary to
CC allow efficient binding of the small ribosomal subunit, and subsequent
CC scanning for the initiator codon (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBUNIT: eIF4F is a multi-subunit complex, the composition of which
CC varies with external and internal environmental conditions. It is
CC composed of at least EIF4A, EIF4E and EIF4G (By similarity).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. eIF4A subfamily.
CC {ECO:0000305}.
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DR EMBL; X79139; CAA55740.1; -; mRNA.
DR PIR; S52021; S52021.
DR AlphaFoldDB; Q40469; -.
DR SMR; Q40469; -.
DR PRIDE; Q40469; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR GO; GO:0002183; P:cytoplasmic translational initiation; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; Initiation factor; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome; RNA-binding.
FT CHAIN <1..>253
FT /note="Eukaryotic initiation factor 4A-6"
FT /id="PRO_0000054953"
FT DOMAIN <1..82
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 93..>253
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 30..33
FT /note="DEAD box"
FT NON_TER 1
FT NON_TER 253
SQ SEQUENCE 253 AA; 29368 MW; 7AD0E1482060E8BA CRC64;
HVVVGTPGRV FDMLRRQSLR PDNIKMFVLD EADEMLSRGF KDQIYDIFQL LPPKIQVGIF
SATMPPEALE ITRKFMNKPV RILVKRDELT LEGIKQFYVN VDKEEWKLET LCDLYETLAI
TQSVIFVNTR RKVDWLTDKM RGRDHTVSAT HGDMDQNTRD IIMREFRSGS SRVLITTDLL
ARGIDVQQVS LVINYDLPTQ PENYLHRIGR SGRFGRKGVS INFVTKDDER MLFDIQKFYN
VVIEELPANV ADL
