IF4A8_TOBAC
ID IF4A8_TOBAC Reviewed; 413 AA.
AC P41381;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Eukaryotic initiation factor 4A-8;
DE Short=eIF-4A-8;
DE EC=3.6.4.13;
DE AltName: Full=ATP-dependent RNA helicase eIF4A-8;
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Samsun; TISSUE=Pollen;
RX PubMed=7727743; DOI=10.1007/bf00020219;
RA Brander K.A., Kuhlemeier C.;
RT "A pollen-specific DEAD-box protein related to translation initiation
RT factor eIF-4A from tobacco.";
RL Plant Mol. Biol. 27:637-649(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Samsun; TISSUE=Leaf;
RX PubMed=7742374; DOI=10.1016/0167-4781(95)00052-i;
RA Brander K.A., Mandel T., Owttrim G.W., Kuhlemeier C.;
RT "Highly conserved genes coding for eukaryotic translation initiation factor
RT eIF-4A of tobacco have specific alterations in functional motifs.";
RL Biochim. Biophys. Acta 1261:442-444(1995).
CC -!- FUNCTION: ATP-dependent RNA helicase which is a subunit of the eIF4F
CC complex involved in cap recognition and is required for mRNA binding to
CC ribosome. In the current model of translation initiation, eIF4A unwinds
CC RNA secondary structures in the 5'-UTR of mRNAs which is necessary to
CC allow efficient binding of the small ribosomal subunit, and subsequent
CC scanning for the initiator codon (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBUNIT: eIF4F is a multi-subunit complex, the composition of which
CC varies with external and internal environmental conditions. It is
CC composed of at least EIF4A, EIF4E and EIF4G (By similarity).
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Pollen specific.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. eIF4A subfamily.
CC {ECO:0000305}.
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DR EMBL; X79004; CAA55639.1; -; mRNA.
DR EMBL; X79005; CAA55640.1; -; Genomic_DNA.
DR PIR; S60244; S60244.
DR RefSeq; NP_001313012.1; NM_001326083.1.
DR AlphaFoldDB; P41381; -.
DR SMR; P41381; -.
DR STRING; 4097.P41381; -.
DR PRIDE; P41381; -.
DR GeneID; 107821770; -.
DR KEGG; nta:107821770; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR GO; GO:0002183; P:cytoplasmic translational initiation; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; Initiation factor; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome; RNA-binding.
FT CHAIN 1..413
FT /note="Eukaryotic initiation factor 4A-8"
FT /id="PRO_0000054955"
FT DOMAIN 71..241
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 252..413
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 40..68
FT /note="Q motif"
FT MOTIF 189..192
FT /note="DEAD box"
FT BINDING 84..91
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 413 AA; 46888 MW; CF79EAD245945044 CRC64;
MARLAPDGAQ FDARQYDSKM NDLLAADGKD FFTSYDEVYD SFDAMGLQEN LLRGIYAYGF
EKPSAIQQRG IVPFCKGLDV IQQAQSGTGK TATFCSGILQ QLDYGLVQCQ ALVLAPTREL
AQQIEKVMRA LGDYLGVKVH ACVGGTSVRE DQRILAAGVH VIVGTPGRVF DMLRRQSLRP
DYLRMFVLDE ADEMLSRGFK DQIYDIFQML PTKVQVGVFS ATMPPEALDI TRKFMNKPVR
ILVKRDELTL EGIKQFYVNV DKEEWKLETL CDLYETLAIT QSVIFVNTRR KVDWLTDKMR
TRDHTVSATH GDMDQNTRDI IMREFRSGSS RVLITTDLLA RGIDVQQVSL VINYDLPTQP
ENYLHRIGRS GRFGRKGVAI NFVTTDDERM LFDIQKFYNV IIEELPSNVA DLL
