ID   IF4A_MAIZE              Reviewed;         410 AA.
AC   Q41741;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Eukaryotic initiation factor 4A;
DE            Short=eIF-4A;
DE            EC=3.6.4.13;
DE   AltName: Full=ATP-dependent RNA helicase eIF4A;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Ear of corn;
RX   PubMed=7630958; DOI=10.1104/pp.108.3.1317;
RA   Jayachandran S., Bailey-Serres J.;
RT   "Nucleotide sequence of a cDNA for the maize protein synthesis initiation
RT   factor 4A.";
RL   Plant Physiol. 108:1317-1318(1995).
CC   -!- FUNCTION: ATP-dependent RNA helicase which is a subunit of the eIF4F
CC       complex involved in cap recognition and is required for mRNA binding to
CC       ribosome. In the current model of translation initiation, eIF4A unwinds
CC       RNA secondary structures in the 5'-UTR of mRNAs which is necessary to
CC       allow efficient binding of the small ribosomal subunit, and subsequent
CC       scanning for the initiator codon (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: eIF4F is a multi-subunit complex, the composition of which
CC       varies with external and internal environmental conditions. It is
CC       composed of at least EIF4A, EIF4E and EIF4G (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. eIF4A subfamily.
CC       {ECO:0000305}.
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DR   EMBL; U17979; AAA82736.1; -; mRNA.
DR   AlphaFoldDB; Q41741; -.
DR   SMR; Q41741; -.
DR   STRING; 4577.GRMZM2G027995_P02; -.
DR   PaxDb; Q41741; -.
DR   PRIDE; Q41741; -.
DR   MaizeGDB; 112980; -.
DR   eggNOG; KOG0327; Eukaryota.
DR   Proteomes; UP000007305; Unplaced.
DR   ExpressionAtlas; Q41741; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IEA:EnsemblPlants.
DR   GO; GO:0005634; C:nucleus; IEA:EnsemblPlants.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR   GO; GO:0002183; P:cytoplasmic translational initiation; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Helicase; Hydrolase; Initiation factor; Nucleotide-binding;
KW   Protein biosynthesis; Reference proteome; RNA-binding.
FT   CHAIN           1..410
FT                   /note="Eukaryotic initiation factor 4A"
FT                   /id="PRO_0000054962"
FT   DOMAIN          68..238
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          249..410
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   MOTIF           37..65
FT                   /note="Q motif"
FT   MOTIF           186..189
FT                   /note="DEAD box"
FT   BINDING         81..88
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   410 AA;  46536 MW;  B9B8AB78ED3CE184 CRC64;
     MAGLAPEGSQ FDDKQYDKKM QEILTEDFFT SYDDVCESFD SMGLQENLLR GIYAYGFEKP
     SAIQQRGIVP FCKGLDVIQQ AQSGTGKTAT FCSGILQQLD YGLVECQALV LAPTRELAQQ
     IEKVMRALGD YLGVKVHACV GGTSVREDQR ILASGVHVVV GTPGRVFDML RRQSLRPDNI
     KMFVLDEADE MLSRGFKDQI YDIFQLLPSK IQVGVFSATM PPEALEITRK FMNKPVRILV
     KRDELTLEGI KQFYVNIDKE DWKLDTLCDL YETLAITQSV IFVNTRRKVD WLTDKMRSRD
     HTVSATHGDM DQNTRDIIMR EFRSGSSRVL ITTDLLARGI DVQQVSLVIN YDLPTQPENY
     LHRIGRSGRF GRKGVAINFV TRDDERIVFD VQRFYNVTVE ELPANVADLL