ID   IF4A_WHEAT              Reviewed;         414 AA.
AC   P41378;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Eukaryotic initiation factor 4A;
DE            Short=eIF-4A;
DE            EC=3.6.4.13;
DE   AltName: Full=ATP-dependent RNA helicase eIF4A;
OS   Triticum aestivum (Wheat).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX   NCBI_TaxID=4565;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8406026; DOI=10.1016/0378-1119(93)90310-y;
RA   Metz A.M., Browning K.S.;
RT   "Sequence of a cDNA encoding wheat eukaryotic protein synthesis initiation
RT   factor 4A.";
RL   Gene 131:299-300(1993).
CC   -!- FUNCTION: ATP-dependent RNA helicase which is a subunit of the eIF4F
CC       complex involved in cap recognition and is required for mRNA binding to
CC       ribosome. In the current model of translation initiation, eIF4A unwinds
CC       RNA secondary structures in the 5'-UTR of mRNAs which is necessary to
CC       allow efficient binding of the small ribosomal subunit, and subsequent
CC       scanning for the initiator codon.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: eIF4F is a multi-subunit complex, the composition of which
CC       varies with external and internal environmental conditions. It is
CC       composed of at least EIF4A, EIF4E and EIF4G (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. eIF4A subfamily.
CC       {ECO:0000305}.
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DR   EMBL; Z21510; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; JN0839; JN0839.
DR   AlphaFoldDB; P41378; -.
DR   SMR; P41378; -.
DR   STRING; 4565.Traes_7DL_6AC3E4622.2; -.
DR   ChEMBL; CHEMBL3308965; -.
DR   PRIDE; P41378; -.
DR   eggNOG; KOG0327; Eukaryota.
DR   Proteomes; UP000019116; Unplaced.
DR   ExpressionAtlas; P41378; baseline and differential.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR   GO; GO:0002183; P:cytoplasmic translational initiation; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Helicase; Hydrolase; Initiation factor; Nucleotide-binding;
KW   Protein biosynthesis; Reference proteome; RNA-binding.
FT   CHAIN           1..414
FT                   /note="Eukaryotic initiation factor 4A"
FT                   /id="PRO_0000054964"
FT   DOMAIN          72..242
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          253..414
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   MOTIF           41..69
FT                   /note="Q motif"
FT   MOTIF           190..193
FT                   /note="DEAD box"
FT   BINDING         85..92
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   414 AA;  46928 MW;  88CFEC8BF56C867F CRC64;
     MAGMAPEGSQ FDAKNYDSKM QELLSQGETE EFFTSYDEVH ESFDDMGLQE NLLRGIYAYG
     FEKPSAIQQR GIVPFCKGLD VIQQAQSGTG KTATFCSGIL QQLDYGLVEC QALVLAPTRE
     LAQQIEKVMR ALGDYLGVKV HACVGGTSVR EDQRILASGV HVVVGTPGRV FDIVRRQSLR
     PDNIKMFVLD EADEMLSRGF KDQIYDIFQL LPGKIQVGVF SATMPPEALE ITRKFMNKPV
     RILVKRDELT LEGIKQFYVN VEKEEWKLDT LCDLYETLAI TQSVIFVNTR RKVDWLTDKM
     RGRDHTVSAT HGDMDQNTRD IIMREFRSGS SRVLITTDLL ARGIDVQQVS LVINYDLPTQ
     PENYQHRIGR SGRFGRKGVA INFVTREDER MLFDIQKFYN VVIEELPANV ADLL