IF4E1_CAEEL
ID IF4E1_CAEEL Reviewed; 212 AA.
AC O45551;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 17-JAN-2003, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Eukaryotic translation initiation factor 4E-1;
DE Short=eIF-4E-1;
DE Short=eIF4E-1;
DE AltName: Full=eIF-4F 25 kDa subunit;
DE AltName: Full=mRNA cap-binding protein;
GN Name=ife-1; ORFNames=F53A2.6;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=Bristol N2;
RX PubMed=9553113; DOI=10.1074/jbc.273.17.10538;
RA Jankowska-Anyszka M., Lamphear B.J., Aamodt E.J., Harrington T.,
RA Darzynkiewicz E., Stolarski R., Rhoads R.E.;
RT "Multiple isoforms of eukaryotic protein synthesis initiation factor 4E in
RT Caenorhabditis elegans can distinguish between mono- and trimethylated mRNA
RT cap structures.";
RL J. Biol. Chem. 273:10538-10542(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH PGL-1, TISSUE
RP SPECIFICITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=Bristol N2;
RX PubMed=11641215; DOI=10.1242/dev.128.20.3899;
RA Amiri A., Keiper B.D., Kawasaki I., Fan Y., Kohara Y., Rhoads R.E.,
RA Strome S.;
RT "An isoform of eIF4E is a component of germ granules and is required for
RT spermatogenesis in C. elegans.";
RL Development 128:3899-3912(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4]
RP FUNCTION.
RC STRAIN=Bristol N2;
RX PubMed=10744754; DOI=10.1074/jbc.275.14.10590;
RA Keiper B.D., Lamphear B.J., Deshpande A.M., Jankowska-Anyszka M.,
RA Aamodt E.J., Blumenthal T., Rhoads R.E.;
RT "Functional characterization of five eIF4E isoforms in Caenorhabditis
RT elegans.";
RL J. Biol. Chem. 275:10590-10596(2000).
RN [5]
RP INTERACTION WITH IFET-1.
RX PubMed=19786575; DOI=10.1083/jcb.200903003;
RA Li W., DeBella L.R., Guven-Ozkan T., Lin R., Rose L.S.;
RT "An eIF4E-binding protein regulates katanin protein levels in C. elegans
RT embryos.";
RL J. Cell Biol. 187:33-42(2009).
CC -!- FUNCTION: Recognizes and binds the 7-methylguanosine-containing mRNA
CC cap during an early step in the initiation of protein synthesis and
CC facilitates ribosome binding by inducing the unwinding of the mRNAs
CC secondary structures. All 5 eIF4E proteins bind monomethyl cap
CC structures. Only ife-1, ife-2 and ife-5 bind trimethyl cap structures
CC which result from trans-splicing. Translation of trimethyl cap
CC structure mRNAs may be regulated by intracellular redox state;
CC disulfide bonds change the width and depth of the cap-binding cavity
CC determining selectivity to mRNA caps. Required for progression through
CC meiotic divisions during spermatogenesis and for the production of
CC viable sperm. It is not required during oogenesis.
CC {ECO:0000269|PubMed:10744754, ECO:0000269|PubMed:11641215,
CC ECO:0000269|PubMed:9553113}.
CC -!- SUBUNIT: EIF4F is a multi-subunit complex, the composition of which
CC varies with external and internal environmental conditions. It is
CC composed of at least EIF4A, EIF4E and EIF4G. EIF4E is also known to
CC interact with other partners, including pgl-1 (PubMed:11641215).
CC Interacts with ifet-1 (PubMed:19786575). {ECO:0000269|PubMed:11641215,
CC ECO:0000269|PubMed:19786575}.
CC -!- INTERACTION:
CC O45551; Q9TZQ3: pgl-1; NbExp=2; IntAct=EBI-330148, EBI-332200;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11641215}.
CC Note=When associated with pgl-1.
CC -!- TISSUE SPECIFICITY: Enriched in the germline from L3 larvae to adults;
CC regions of the gonad undergoing spermatogenesis. Expressed in germ
CC granules (P granules); when associated with pgl-1.
CC {ECO:0000269|PubMed:11641215}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Ubiquitous embryonic expression until the 100-200 cell stage then
CC disappears by the 300-400 cell stage. Expressed again in L3 larvae
CC through to adulthood. {ECO:0000269|PubMed:11641215}.
CC -!- DISRUPTION PHENOTYPE: Defects can cause a delay in spermatogenesis,
CC temperature-sensitive sterility and defective sperm.
CC {ECO:0000269|PubMed:11641215}.
CC -!- SIMILARITY: Belongs to the eukaryotic initiation factor 4E family.
CC {ECO:0000305}.
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DR EMBL; Z81546; CAB04454.2; -; Genomic_DNA.
DR PIR; T22530; T22530.
DR RefSeq; NP_001255197.1; NM_001268268.1.
DR AlphaFoldDB; O45551; -.
DR SMR; O45551; -.
DR BioGRID; 41924; 7.
DR IntAct; O45551; 4.
DR STRING; 6239.F53A2.6a; -.
DR iPTMnet; O45551; -.
DR EPD; O45551; -.
DR PaxDb; O45551; -.
DR PeptideAtlas; O45551; -.
DR EnsemblMetazoa; F53A2.6b.1; F53A2.6b.1; WBGene00002059.
DR GeneID; 176755; -.
DR UCSC; F53A2.6.2; c. elegans.
DR CTD; 176755; -.
DR WormBase; F53A2.6b; CE31809; WBGene00002059; ife-1.
DR eggNOG; KOG1670; Eukaryota.
DR GeneTree; ENSGT00940000173082; -.
DR HOGENOM; CLU_043552_1_0_1; -.
DR InParanoid; O45551; -.
DR PhylomeDB; O45551; -.
DR PRO; PR:O45551; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00002059; Expressed in reproductive system and 6 other tissues.
DR ExpressionAtlas; O45551; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:WormBase.
DR GO; GO:0016281; C:eukaryotic translation initiation factor 4F complex; IBA:GO_Central.
DR GO; GO:0043186; C:P granule; IDA:WormBase.
DR GO; GO:0000340; F:RNA 7-methylguanosine cap binding; IDA:WormBase.
DR GO; GO:0000341; F:RNA trimethylguanosine cap binding; IDA:WormBase.
DR GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IGI:WormBase.
DR GO; GO:0001556; P:oocyte maturation; IMP:WormBase.
DR GO; GO:0007286; P:spermatid development; IMP:WormBase.
DR Gene3D; 3.30.760.10; -; 1.
DR InterPro; IPR023398; TIF_eIF4e-like.
DR InterPro; IPR001040; TIF_eIF_4E.
DR InterPro; IPR019770; TIF_eIF_4E_CS.
DR PANTHER; PTHR11960; PTHR11960; 1.
DR Pfam; PF01652; IF4E; 1.
DR SUPFAM; SSF55418; SSF55418; 1.
DR PROSITE; PS00813; IF4E; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Differentiation; Disulfide bond;
KW Initiation factor; Protein biosynthesis; Reference proteome; RNA-binding;
KW Spermatogenesis; Translation regulation.
FT CHAIN 1..212
FT /note="Eukaryotic translation initiation factor 4E-1"
FT /id="PRO_0000193643"
FT DISULFID 125..129
FT /evidence="ECO:0000250"
SQ SEQUENCE 212 AA; 24314 MW; D5BC1BBC74F3AC2F CRC64;
MTETEQTTAP IYPLKRNWTW WYLNDERNKS WEDRLKKVYT FNTVSEFWAL YDAIRPPSGL
NALCDYNVFR DDIQPMWEVP ENSNGGRWLI VIDKGKTPEM VDAIWLEILM ALVGEQFGKD
MESICGLVCN VRGKGSKISV WTKDCNDDET NMRIGVVLKE KLMAASKDHS KPLFDVIRYE
DHESCQKKTS SVVKAKLSLH SSDAPVAEKS AV
