IF4E1_CAPAN
ID IF4E1_CAPAN Reviewed; 228 AA.
AC A0A1U8GR65; A7XZS4; A7XZS6; A7XZS7; I0J1J4; I2FKR0; I2FKR3; I2FKR5; Q2TPB0;
AC Q2TPB1; Q2TPB2; Q2TPB3; Q6RZ28; Q6RZ30; Q8GSF9; Q8H6L5; Q8H6L6;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 23-FEB-2022, sequence version 2.
DT 03-AUG-2022, entry version 16.
DE RecName: Full=Eukaryotic translation initiation factor 4E-1 {ECO:0000303|PubMed:18182024};
DE Short=eIF4E-1 {ECO:0000303|PubMed:18182024};
DE AltName: Full=eIF-4F 25 kDa subunit {ECO:0000305};
DE AltName: Full=eIF-4F p26 subunit {ECO:0000305};
DE AltName: Full=mRNA cap-binding protein {ECO:0000305};
GN Name=eIF4E {ECO:0000303|PubMed:18182024};
GN Synonyms=PVR1 {ECO:0000303|PubMed:15842624},
GN PVR2 {ECO:0000303|PubMed:18182024};
GN ORFNames=LOC107868427 {ECO:0000312|RefSeq:XP_016570599.1},
GN T459_11130 {ECO:0000312|EMBL:PHT82687.1};
OS Capsicum annuum (Capsicum pepper).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Capsiceae; Capsicum.
OX NCBI_TaxID=4072;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], VARIANTS THR-66; 68-ALA--ALA-74 DELINS
RP GLU-LYS-SER-LYS-GLN-ASP-ASP; ASP-73; GLY-205 AND ASN-219, AND POLYMORPHISM.
RC STRAIN=cv. Maroc1, cv. Perennial, cv. PI 322719, and cv. SC81;
RA Ruffel S., Moretti A., Palloix A., Lesage M.-L., Caranta C.;
RT "Amino acid polymorphism in the eukaryotic translation initiation factor 4E
RT determines resistance against several potyviruses in tomato and pepper.";
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, FUNCTION (MICROBIAL INFECTION),
RP VARIANTS GLU-67; ARG-79; ASN-109 AND GLY-205, AND POLYMORPHISM.
RC STRAIN=cv. Doux Long des Landes, cv. Florida VR2, cv. Yolo Wonder, and
RC cv. Yolo Y;
RX PubMed=12492847; DOI=10.1046/j.1365-313x.2002.01499.x;
RA Ruffel S., Dussault M.-H., Palloix A., Moury B., Bendahmane A.,
RA Robaglia C., Caranta C.;
RT "A natural recessive resistance gene against potato virus Y in pepper
RT corresponds to the eukaryotic initiation factor 4E (eIF4E).";
RL Plant J. 32:1067-1075(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLU-67 AND GLY-205, AND
RP POLYMORPHISM.
RC STRAIN=cv. Yolo Wonder/HD208;
RX PubMed=15315824; DOI=10.1016/j.gene.2004.05.010;
RA Ruffel S., Caranta C., Palloix A., Lefebvre V., Caboche M., Bendahmane A.;
RT "Structural analysis of the eukaryotic initiation factor 4E gene
RT controlling potyvirus resistance in pepper: exploitation of a BAC
RT library.";
RL Gene 338:209-216(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, FUNCTION (MICROBIAL INFECTION),
RP VARIANTS ALA-51; THR-66; GLU-67; ARG-71; ARG-79; ARG-107; ASN-109 AND
RP GLY-205, INTERACTION WITH VIRAL VPG (MICROBIAL INFECTION), AND
RP POLYMORPHISM.
RC STRAIN=cv. Dempsey, cv. ECW, cv. PI159234, and cv. Yolo Y;
RX PubMed=15842624; DOI=10.1111/j.1365-313x.2005.02381.x;
RA Kang B.-C., Yeam I., Frantz J.D., Murphy J.F., Jahn M.M.;
RT "The pvr1 locus in Capsicum encodes a translation initiation factor eIF4E
RT that interacts with Tobacco etch virus VPg.";
RL Plant J. 42:392-405(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA], VARIANTS GLU-68; 68-ALA--ALA-73 DELINS
RP GLU-LYS-SER-LYS-GLN-ASP; ARG-79; ARG-107 AND GLY-205, FUNCTION, FUNCTION
RP (MICROBIAL INFECTION), INTERACTION WITH VIRAL VPG (MICROBIAL INFECTION),
RP AND POLYMORPHISM.
RC STRAIN=cv. Antibois, cv. Bastidon, cv. Bousso 1, cv. Chay Angolano,
RC cv. Chile de Arbol, cv. Cuba 3, cv. Flambeau, cv. Florida VR2, cv. H3,
RC cv. HD-C69, cv. Lamu, cv. LP1, cv. Maroc 1, cv. Perennial, cv. PI187331,
RC cv. PI195301, cv. PI201234, cv. PI322719, cv. Pimiento Morron, cv. SC81,
RC cv. Serrano Vera Cruz, and cv. Yolo Y;
RX PubMed=18182024; DOI=10.1111/j.1365-313x.2008.03407.x;
RA Charron C., Nicolai M., Gallois J.-L., Robaglia C., Moury B., Palloix A.,
RA Caranta C.;
RT "Natural variation and functional analyses provide evidence for co-
RT evolution between plant eIF4E and potyviral VPg.";
RL Plant J. 54:56-68(2008).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. CM334;
RX PubMed=24441736; DOI=10.1038/ng.2877;
RA Kim S., Park M., Yeom S.I., Kim Y.M., Lee J.M., Lee H.A., Seo E., Choi J.,
RA Cheong K., Kim K.T., Jung K., Lee G.W., Oh S.K., Bae C., Kim S.B.,
RA Lee H.Y., Kim S.Y., Kim M.S., Kang B.C., Jo Y.D., Yang H.B., Jeong H.J.,
RA Kang W.H., Kwon J.K., Shin C., Lim J.Y., Park J.H., Huh J.H., Kim J.S.,
RA Kim B.D., Cohen O., Paran I., Suh M.C., Lee S.B., Kim Y.K., Shin Y.,
RA Noh S.J., Park J., Seo Y.S., Kwon S.Y., Kim H.A., Park J.M., Kim H.J.,
RA Choi S.B., Bosland P.W., Reeves G., Jo S.H., Lee B.W., Cho H.T., Choi H.S.,
RA Lee M.S., Yu Y., Do Choi Y., Park B.S., van Deynze A., Ashrafi H., Hill T.,
RA Kim W.T., Pai H.S., Ahn H.K., Yeam I., Giovannoni J.J., Rose J.K.,
RA Soerensen I., Lee S.J., Kim R.W., Choi I.Y., Choi B.S., Lim J.S., Lee Y.H.,
RA Choi D.;
RT "Genome sequence of the hot pepper provides insights into the evolution of
RT pungency in Capsicum species.";
RL Nat. Genet. 46:270-278(2014).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Zunla-1;
RX PubMed=24591624; DOI=10.1073/pnas.1400975111;
RA Qin C., Yu C., Shen Y., Fang X., Chen L., Min J., Cheng J., Zhao S., Xu M.,
RA Luo Y., Yang Y., Wu Z., Mao L., Wu H., Ling-Hu C., Zhou H., Lin H.,
RA Gonzalez-Morales S., Trejo-Saavedra D.L., Tian H., Tang X., Zhao M.,
RA Huang Z., Zhou A., Yao X., Cui J., Li W., Chen Z., Feng Y., Niu Y., Bi S.,
RA Yang X., Li W., Cai H., Luo X., Montes-Hernandez S., Leyva-Gonzalez M.A.,
RA Xiong Z., He X., Bai L., Tan S., Tang X., Liu D., Liu J., Zhang S.,
RA Chen M., Zhang L., Zhang L., Zhang Y., Liao W., Zhang Y., Wang M., Lv X.,
RA Wen B., Liu H., Luan H., Zhang Y., Yang S., Wang X., Xu J., Li X., Li S.,
RA Wang J., Palloix A., Bosland P.W., Li Y., Krogh A., Rivera-Bustamante R.F.,
RA Herrera-Estrella L., Yin Y., Yu J., Hu K., Zhang Z.;
RT "Whole-genome sequencing of cultivated and wild peppers provides insights
RT into Capsicum domestication and specialization.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:5135-5140(2014).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. CM334;
RX PubMed=29089032; DOI=10.1186/s13059-017-1341-9;
RA Kim S., Park J., Yeom S.I., Kim Y.M., Seo E., Kim K.T., Kim M.S., Lee J.M.,
RA Cheong K., Shin H.S., Kim S.B., Han K., Lee J., Park M., Lee H.A.,
RA Lee H.Y., Lee Y., Oh S., Lee J.H., Choi E., Choi E., Lee S.E., Jeon J.,
RA Kim H., Choi G., Song H., Lee J., Lee S.C., Kwon J.K., Lee H.Y., Koo N.,
RA Hong Y., Kim R.W., Kang W.H., Huh J.H., Kang B.C., Yang T.J., Lee Y.H.,
RA Bennetzen J.L., Choi D.;
RT "New reference genome sequences of hot pepper reveal the massive evolution
RT of plant disease-resistance genes by retroduplication.";
RL Genome Biol. 18:R210.1-R210.11(2017).
RN [9]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 7-228, FUNCTION, FUNCTION (MICROBIAL
RP INFECTION), VARIANTS THR-15; VAL-15; ASP-65; THR-66; GLU-67; ARG-71;
RP ASP-73; THR-73; ASP-74; ILE-77; ARG-79; ARG-107 ASN-109; ILE-131; GLY-160;
RP GLY-205; HIS-213; GLU-214; ALA-216; ARG-218; PHE-218; ASN-219 AND SER-221,
RP AND POLYMORPHISM.
RC STRAIN=cv. CDP00620, cv. CDP04928, cv. CDP06188, and cv. CDP06433;
RC TISSUE=Leaf;
RX PubMed=21073716; DOI=10.1186/1471-2164-11-631;
RA Ibiza V.P., Canizares J., Nuez F.;
RT "EcoTILLING in Capsicum species: searching for new virus resistances.";
RL BMC Genomics 11:631-631(2010).
RN [10]
RP FUNCTION, AND FUNCTION (MICROBIAL INFECTION).
RX PubMed=15971038; DOI=10.1007/s00438-005-0003-x;
RA Ruffel S., Gallois J.L., Lesage M.L., Caranta C.;
RT "The recessive potyvirus resistance gene pot-1 is the tomato orthologue of
RT the pepper pvr2-eIF4E gene.";
RL Mol. Genet. Genomics 274:346-353(2005).
RN [11]
RP FUNCTION, AND FUNCTION (MICROBIAL INFECTION).
RC STRAIN=cv. Florida VR2, cv. Yolo Wonder, and cv. Yolo Y;
RX PubMed=16760413; DOI=10.1099/vir.0.81817-0;
RA Ruffel S., Gallois J.-L., Moury B., Robaglia C., Palloix A., Caranta C.;
RT "Simultaneous mutations in translation initiation factors eIF4E and
RT eIF(iso)4E are required to prevent pepper veinal mottle virus infection of
RT pepper.";
RL J. Gen. Virol. 87:2089-2098(2006).
RN [12]
RP GENE FAMILY, AND REVIEW.
RX PubMed=24309680; DOI=10.1016/j.meegid.2013.11.024;
RA Moury B., Charron C., Janzac B., Simon V., Gallois J.L., Palloix A.,
RA Caranta C.;
RT "Evolution of plant eukaryotic initiation factor 4E (eIF4E) and potyvirus
RT genome-linked protein (VPg): a game of mirrors impacting resistance
RT spectrum and durability.";
RL Infect. Genet. Evol. 27:472-480(2014).
CC -!- FUNCTION: Component of the protein complex eIF4F, which is involved in
CC the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal
CC secondary structure and recruitment of mRNA to the ribosome
CC (PubMed:18182024). Recognizes and binds the 7-methylguanosine-
CC containing mRNA cap during an early step in the initiation of protein
CC synthesis and facilitates ribosome binding by inducing the unwinding of
CC the mRNAs secondary structures (PubMed:18182024). Key component of
CC recessive resistance to potyviruses (PubMed:15971038, PubMed:12492847,
CC PubMed:15842624, PubMed:18182024, PubMed:16760413, PubMed:21073716).
CC {ECO:0000269|PubMed:12492847, ECO:0000269|PubMed:15842624,
CC ECO:0000269|PubMed:15971038, ECO:0000269|PubMed:16760413,
CC ECO:0000269|PubMed:18182024, ECO:0000269|PubMed:21073716}.
CC -!- FUNCTION: (Microbial infection) Susceptibility host factor required for
CC viral infection (e.g. potato virus Y (PVY) and tobacco etch virus
CC (TEV)) by recruiting viral RNAs to the host ribosomal complex via an
CC interaction with viral genome-linked protein (VPg).
CC {ECO:0000269|PubMed:12492847, ECO:0000269|PubMed:15842624,
CC ECO:0000269|PubMed:15971038, ECO:0000269|PubMed:16760413,
CC ECO:0000269|PubMed:18182024, ECO:0000269|PubMed:21073716}.
CC -!- SUBUNIT: EIF4F is a multi-subunit complex, the composition of which
CC varies with external and internal environmental conditions. It is
CC composed of at least EIF4A, EIF4E and EIF4G. EIF4E is also known to
CC interact with other partners. In higher plants two isoforms of EIF4F
CC have been identified, named isoform EIF4F and isoform EIF(iso)4F.
CC Isoform EIF4F has subunits p220 and p26, whereas isoform EIF(iso)4F has
CC subunits p82 and p28. {ECO:0000250|UniProtKB:P29557}.
CC -!- SUBUNIT: (Microbial infection) Interacts with potyvirus viral genome-
CC linked protein (VPg); this interaction is possible in susceptible hosts
CC but is impaired in resistant plants (PubMed:15842624, PubMed:18182024).
CC Thus the VPg of tobacco etch virus (TEV) strain HAT interacts with
CC susceptible alleles pvr2(+), pvr2(3) and pvr2(9) but not with the
CC resistant allele pvr2(2), the VPg of TEV strain CAA10 interacts with
CC susceptible alleles pvr2(+), pvr2(2), pvr2(3) and pvr2(9), the VPg of
CC potato virus Y (PVY) strain LYE84 interacts with tomato eIF4E1 and
CC eIF4E2 as well as with the Capsicum annuum eIF4E1 susceptible allele
CC pvr2(+) but not with resistant alleles pvr2(1), pvr2(2), pvr2(3),
CC pvr2(4), pvr2(5), pvr2(6), pvr2(7), pvr2(8) and pvr2(9) and the VPg of
CC PVY strain SON41 interacts with C.annuum eIF4E1 susceptible alleles
CC pvr2(+), pvr2(1), pvr2(2), pvr2(3) and pvr2(4) but not with resistant
CC alleles pvr2(5), pvr2(6), pvr2(7), pvr2(8) and pvr2(9)
CC (PubMed:18182024). In addition, the susceptible allele pvr1(+)
CC interacts strongly with TEV strains HAT and NW VPg while resistance
CC alleles (pvr1, pvr1(1), and pvr1(2)) fail to bind TEV VPg
CC (PubMed:15842624). {ECO:0000269|PubMed:15842624,
CC ECO:0000269|PubMed:18182024}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:C6ZJZ3}. Cytoplasm
CC {ECO:0000250|UniProtKB:C6ZJZ3}.
CC -!- PTM: According to the redox status, the Cys-126-Cys-164 disulfide
CC bridge may have a role in regulating protein function by affecting its
CC ability to bind capped mRNA. {ECO:0000250|UniProtKB:P29557}.
CC -!- POLYMORPHISM: Variant present in the strains cv. Serrano Vera Cruz,
CC allele pvr2(7), confers an increased resistance to potato virus Y (PVY)
CC strains LYE84 and SON41 but sensitivity to tobacco etch virus (TEV)
CC strains HAT and CAA10. {ECO:0000269|PubMed:18182024}.
CC -!- POLYMORPHISM: Variant present in the strains cv. PI195301, allele
CC pvr2(8), confers an increased resistance to potato virus Y (PVY)
CC strains LYE84 and SON41 but sensitivity to tobacco etch virus (TEV)
CC strains HAT and CAA10. {ECO:0000269|PubMed:18182024}.
CC -!- POLYMORPHISM: Variant present in the strains cv. Chile de Arbol, allele
CC pvr2(9), confers an increased resistance to potato virus Y (PVY)
CC strains LYE84 and SON41 but sensitivity to tobacco etch virus (TEV)
CC strains HAT and CAA10. {ECO:0000269|PubMed:18182024}.
CC -!- POLYMORPHISM: Variant present in the strains cv. Maroc 1 and LP1,
CC allele pvr2(6), confers an increased resistance to potato virus Y (PVY)
CC strains LYE84 and SON41 but sensitivity to tobacco etch virus (TEV)
CC strains HAT and CAA10. {ECO:0000269|Ref.1}.
CC -!- POLYMORPHISM: Variant present in the strains cv. Florida VR2, cv.
CC Dempsey, cv. CDP06188 and cv. Chay Angolano, alleles pvr1-2 and
CC pvr2(2), confers an increased resistance to potato virus Y (PVY) strain
CC LYE84 and to tobacco etch virus (TEV) strains HAT and NW associated
CC with impaired TEV VPg binding, but sensitivity to PVY strain SON41 and
CC to TEV strains CAA10. {ECO:0000269|PubMed:12492847,
CC ECO:0000269|PubMed:15842624, ECO:0000269|PubMed:21073716}.
CC -!- POLYMORPHISM: Variant present in the strains cv. HD-C69, cv. PI201234
CC and cv. Perennial, allele pvr2(3), is associated with an increased
CC resistance to potato virus Y (PVY) strain LYE84 but susceptibility to
CC PVY strain SON41 and to tobacco etch virus (TEV) strains HAT and CAA10.
CC {ECO:0000269|PubMed:12492847, ECO:0000269|PubMed:15315824,
CC ECO:0000269|PubMed:15842624, ECO:0000269|PubMed:18182024,
CC ECO:0000269|PubMed:21073716, ECO:0000269|Ref.1}.
CC -!- POLYMORPHISM: Variant present in the strains cv. Yolo Y and cv.
CC CDP06433, alleles pvr1-1 and pvr2(1), is associated with an increased
CC resistance to potato virus Y (PVY) strain LYE84 but sensitivity to PVY
CC strain SON41 and to tobacco etch virus (TEV) strains HAT and CAA10.
CC {ECO:0000269|PubMed:12492847, ECO:0000269|PubMed:15842624,
CC ECO:0000269|PubMed:21073716}.
CC -!- POLYMORPHISM: Variant present in the allele pvr1, haplotype I1, confers
CC an increased resistance to tobacco etch virus (TEV) strains HAT and NW
CC associated with impaired TEV VPg binding and inability to bind 7-
CC methylguanosine-containing mRNA cap. {ECO:0000269|PubMed:15842624}.
CC -!- POLYMORPHISM: Variant present in the strain cv. SC81, allele pvr2(5),
CC is associated with an increased resistance to potato virus Y (PVY)
CC strains LYE84 and SON41 but sensitivity to tobacco etch virus (TEV)
CC strains HAT and CAA10. {ECO:0000269|Ref.1}.
CC -!- MISCELLANEOUS: Displayed sequence is allele pvr2(+), haplotype B1 found
CC in strains cv. Doux Long des Landes, cv. Yolo Wonder, cv. Flambeau, cv.
CC PI187331, cv. Cuba 3, cv. Bousso 1, cv. Pimiento Morron, cv. H3, cv.
CC Lamu, cv. Bastidon, cv. CDP04928 and cv. Antibois which confers
CC sensitivity to potato virus Y (PVY) strains LYE84 and SON41 and to
CC tobacco etch virus (TEV) strains HAT and CAA10. {ECO:0000305|Ref.1}.
CC -!- MISCELLANEOUS: The allele pvr2(2) correlates with resistance to pepper
CC veinal mottle virus (PVMV), especially when associated with the pvr6
CC allele of eIF(iso)4E (PubMed:16760413). Plants harboring specific
CC haplotypes couples for eIF4E/eIF(iso)4E are resistant or tolerant to
CC potyvirus such as potato virus Y (PVY) (PubMed:21073716).
CC {ECO:0000269|PubMed:16760413, ECO:0000269|PubMed:21073716}.
CC -!- SIMILARITY: Belongs to the eukaryotic initiation factor 4E family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY723737; AAV88614.1; -; mRNA.
DR EMBL; AY723738; AAV88615.1; -; mRNA.
DR EMBL; AY723739; AAV88616.1; -; mRNA.
DR EMBL; AY723740; AAV88617.1; -; mRNA.
DR EMBL; AY122052; AAM82190.1; -; mRNA.
DR EMBL; AF521963; AAN74644.1; -; mRNA.
DR EMBL; AF521964; AAN74645.1; -; mRNA.
DR EMBL; AF521965; AAN74646.1; -; mRNA.
DR EMBL; AH013730; AAS68034.1; -; Genomic_DNA.
DR EMBL; AY485127; AAR23916.1; -; mRNA.
DR EMBL; AY485129; AAR23918.1; -; mRNA.
DR EMBL; AY485130; AAR23919.1; -; mRNA.
DR EMBL; AY485131; AAR23920.1; -; mRNA.
DR EMBL; EU106863; ABU97503.1; -; mRNA.
DR EMBL; EU106864; ABU97504.1; -; mRNA.
DR EMBL; EU106865; ABU97505.1; -; mRNA.
DR EMBL; AYRZ02000004; PHT82687.1; -; Genomic_DNA.
DR EMBL; FN813352; CBL81073.1; -; mRNA.
DR EMBL; FN824321; CBL94657.1; -; mRNA.
DR EMBL; FN824324; CBL94660.1; -; mRNA.
DR EMBL; FN824326; CBL94662.1; -; mRNA.
DR RefSeq; XP_016570599.1; XM_016715113.1.
DR SMR; A0A1U8GR65; -.
DR STRING; 4072.Q2TPB3; -.
DR EnsemblPlants; PHT82687; PHT82687; T459_11130.
DR GeneID; 107868427; -.
DR Gramene; PHT82687; PHT82687; T459_11130.
DR OMA; WHDLLLC; -.
DR OrthoDB; 1394271at2759; -.
DR Proteomes; UP000189700; Chromosome 4.
DR Proteomes; UP000222542; Chromosome 4.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IDA:UniProtKB.
DR GO; GO:0003743; F:translation initiation factor activity; IDA:UniProtKB.
DR GO; GO:0051607; P:defense response to virus; IMP:UniProtKB.
DR GO; GO:0006413; P:translational initiation; IDA:UniProtKB.
DR Gene3D; 3.30.760.10; -; 1.
DR InterPro; IPR023398; TIF_eIF4e-like.
DR InterPro; IPR001040; TIF_eIF_4E.
DR PANTHER; PTHR11960; PTHR11960; 1.
DR Pfam; PF01652; IF4E; 1.
DR SUPFAM; SSF55418; SSF55418; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Disulfide bond; Host-virus interaction; Initiation factor;
KW Nucleus; Plant defense; Protein biosynthesis; Reference proteome;
KW RNA-binding; Translation regulation.
FT CHAIN 1..228
FT /note="Eukaryotic translation initiation factor 4E-1"
FT /id="PRO_0000454063"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 53..56
FT /note="EIF4G-binding"
FT /evidence="ECO:0000250|UniProtKB:Q00LS8"
FT REGION 63..99
FT /note="EIF4G-binding"
FT /evidence="ECO:0000250|UniProtKB:Q00LS8"
FT REGION 147..156
FT /note="EIF4G-binding"
FT /evidence="ECO:0000250|UniProtKB:Q00LS8"
FT COMPBIAS 9..25
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 71..76
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT /ligand_part_id="ChEBI:CHEBI:74429"
FT /ligand_part_note="m7GTP residue in mRNA cap"
FT /evidence="ECO:0000250|UniProtKB:P29557"
FT BINDING 103
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT /ligand_part_id="ChEBI:CHEBI:74429"
FT /ligand_part_note="m7GTP residue in mRNA cap"
FT /evidence="ECO:0000250|UniProtKB:P29557"
FT BINDING 121..122
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT /ligand_part_id="ChEBI:CHEBI:74429"
FT /ligand_part_note="m7GTP residue in mRNA cap"
FT /evidence="ECO:0000250|UniProtKB:P29557"
FT BINDING 171..176
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT /ligand_part_id="ChEBI:CHEBI:74429"
FT /ligand_part_note="m7GTP residue in mRNA cap"
FT /evidence="ECO:0000250|UniProtKB:P29557"
FT BINDING 216..220
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT /ligand_part_id="ChEBI:CHEBI:74429"
FT /ligand_part_note="m7GTP residue in mRNA cap"
FT /evidence="ECO:0000250|UniProtKB:Q00LS8"
FT DISULFID 126..164
FT /evidence="ECO:0000250|UniProtKB:P29557"
FT VARIANT 15
FT /note="A -> T (in allele pvr2(15), haplotype C1)"
FT /evidence="ECO:0000269|PubMed:21073716"
FT VARIANT 15
FT /note="A -> V (in allele pvr2(17), haplotype R1)"
FT /evidence="ECO:0000269|PubMed:21073716"
FT VARIANT 51
FT /note="T -> A (in allele pvr1, haplotype I1. In allele
FT pvr2(21), haplotype P1)"
FT /evidence="ECO:0000269|PubMed:15842624,
FT ECO:0000269|PubMed:21073716"
FT VARIANT 65
FT /note="N -> D (in allele pvr2(13), haplotype N1. In allele
FT pvr2(14), haplotype O1. In allele pvr2(11), haplotype H1.
FT In allele pvr2(12), haplotype K1. In haplotype Sh)"
FT /evidence="ECO:0000269|PubMed:21073716"
FT VARIANT 66
FT /note="P -> T (in strain: SC81, allele pvr2(5). In allele
FT pvr1, haplotype I1. In allele pvr2(10), haplotype D1)"
FT /evidence="ECO:0000269|PubMed:15842624,
FT ECO:0000269|PubMed:21073716, ECO:0000269|Ref.1"
FT VARIANT 67
FT /note="V -> E (in strain: Yolo Y and CDP06433; alleles
FT pvr1-1 and pvr2(1). In strain: HD-C69; PI201234 and
FT Perennial; allele pvr2(3). In strain: Florida VR2; Dempsey;
FT CDP06188 and Chay Angolano; alleles pvr1-2 and pvr2(2))"
FT /evidence="ECO:0000269|PubMed:12492847,
FT ECO:0000269|PubMed:15315824, ECO:0000269|PubMed:15842624,
FT ECO:0000269|PubMed:21073716"
FT VARIANT 68..74
FT /note="AKSKQAA -> VEKSKQDD (in strain: Maroc 1 and LP1,
FT allele pvr2(6))"
FT /evidence="ECO:0000269|Ref.1"
FT VARIANT 68..73
FT /note="AKSKQA -> EKSKQD (in strain: Chile de Arbol, allele
FT pvr2(9))"
FT /evidence="ECO:0000269|PubMed:18182024"
FT VARIANT 68
FT /note="A -> E (in strain: PI195301, allele pvr2(8))"
FT /evidence="ECO:0000269|PubMed:18182024"
FT VARIANT 71
FT /note="K -> R (in strain: CDP00620, allele pvr1(+),
FT haplotypes F1 and F2. In allele pvr2(17), haplotype R1. In
FT allele pvr2(15), haplotype C1. In allele pvr2(22),
FT haplotype Q1. In allele pvr2(16), haplotype G1. In allele
FT pvr2(19), haplotype L1. In allele pvr2(18), haplotype J1.
FT In allele pvr2(13), haplotype N1. In allele pvr2(14),
FT haplotype O1. In allele pvr2(11), haplotype H1. In allele
FT pvr2(12), haplotype K1. In haplotype Sh. In allele
FT pvr2(20), haplotype M1. In allele pvr1, haplotype I1. In
FT allele pvr2(10), haplotype D1. In allele pvr2(21),
FT haplotype P1)"
FT /evidence="ECO:0000269|PubMed:15842624,
FT ECO:0000269|PubMed:21073716"
FT VARIANT 73
FT /note="A -> D (in strain: SC81, allele pvr2(5). In allele
FT pvr2(12), haplotype K1. In allele pvr2(10), haplotype D1)"
FT /evidence="ECO:0000269|PubMed:21073716, ECO:0000269|Ref.1"
FT VARIANT 73
FT /note="A -> T (in allele pvr2(20))"
FT /evidence="ECO:0000269|PubMed:21073716"
FT VARIANT 74
FT /note="A -> D (in allele pvr2(13), haplotype N1. In
FT haplotype Sh)"
FT /evidence="ECO:0000269|PubMed:21073716"
FT VARIANT 77
FT /note="S -> I (in allele pvr2(22), haplotype Q1)"
FT /evidence="ECO:0000269|PubMed:21073716"
FT VARIANT 79
FT /note="L -> R (in strain: Yolo Y and CDP06433; alleles
FT pvr1-1 and pvr2(1). In strain: Florida VR2; Dempsey;
FT CDP06188 and Chay Angolano; alleles pvr1-2 and pvr2(2). In
FT strain: Serrano Vera Cruz; allele pvr2(7). In allele
FT pvr2(19))"
FT /evidence="ECO:0000269|PubMed:12492847,
FT ECO:0000269|PubMed:15842624, ECO:0000269|PubMed:18182024,
FT ECO:0000269|PubMed:21073716"
FT VARIANT 107
FT /note="G -> R (in allele pvr1, haplotype I1. In allele
FT pvr2(8). In allele pvr2(10), haplotype D1)"
FT /evidence="ECO:0000269|PubMed:15842624,
FT ECO:0000269|PubMed:18182024, ECO:0000269|PubMed:21073716"
FT VARIANT 109
FT /note="D -> N (in strain: Florida VR2; CDP06188; Dempsey
FT and Chay Angolano; alleles pvr1-2 and pvr2(2))"
FT /evidence="ECO:0000269|PubMed:12492847,
FT ECO:0000269|PubMed:15842624, ECO:0000269|PubMed:21073716"
FT VARIANT 131
FT /note="T -> I (in allele pvr2(16), haplotype G1. In
FT haplotype Sh)"
FT /evidence="ECO:0000269|PubMed:21073716"
FT VARIANT 160
FT /note="E -> G (in allele pvr2(18), haplotype J1)"
FT /evidence="ECO:0000269|PubMed:21073716"
FT VARIANT 205
FT /note="D -> G (in strain: HD-C69, PI201234 and Perennial,
FT allele pvr2(3))"
FT /evidence="ECO:0000269|PubMed:12492847,
FT ECO:0000269|PubMed:15315824, ECO:0000269|PubMed:15842624,
FT ECO:0000269|PubMed:18182024, ECO:0000269|PubMed:21073716,
FT ECO:0000269|Ref.1"
FT VARIANT 213
FT /note="D -> H (in allele pvr2(11), haplotype H1)"
FT /evidence="ECO:0000269|PubMed:21073716"
FT VARIANT 214
FT /note="D -> E (in allele pvr2(18), haplotype J1)"
FT /evidence="ECO:0000269|PubMed:21073716"
FT VARIANT 216
FT /note="K -> A (in allele pvr2(18), haplotype J1)"
FT /evidence="ECO:0000269|PubMed:21073716"
FT VARIANT 218
FT /note="L -> F (in allele pvr2(10), haplotype D1)"
FT /evidence="ECO:0000269|PubMed:21073716"
FT VARIANT 218
FT /note="L -> R (in allele pvr2(11), haplotype H1)"
FT /evidence="ECO:0000269|PubMed:21073716"
FT VARIANT 219
FT /note="D -> N (in strain: SC81, allele pvr2(5). In allele
FT pvr2(18), haplotype J1)"
FT /evidence="ECO:0000269|PubMed:21073716, ECO:0000269|Ref.1"
FT VARIANT 221
FT /note="N -> S (in haplotype Sh)"
FT /evidence="ECO:0000269|PubMed:21073716"
SQ SEQUENCE 228 AA; 25908 MW; 258E1F2AA30F795A CRC64;
MATAEMEKTT TFDEAEKVKL NANEADDEVE EGEIVEETDD TTSYLSKEIA TKHPLEHSWT
FWFDNPVAKS KQAAWGSSLR NVYTFSTVED FWGAYNNIHH PSKLVVGADL HCFKHKIEPK
WEDPVCANGG TWKMSFSKGK SDTSWLYTLL AMIGHQFDHE DEICGAVVSV RGKGEKISLW
TKNAANETAQ VSIGKQWKQF LDYSDSVGFI FHDDAKRLDR NAKNRYTV
