IF4E1_LACSA
ID IF4E1_LACSA Reviewed; 235 AA.
AC A0A2J6L8Y7; Q7XJB1;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 28-MAR-2018, sequence version 1.
DT 03-AUG-2022, entry version 17.
DE RecName: Full=Eukaryotic translation initiation factor 4E-1 {ECO:0000303|PubMed:12857809};
DE Short=Ls-eIF4E-1 {ECO:0000303|PubMed:12857809};
DE Short=eIF-4E-1 {ECO:0000303|PubMed:12857809};
DE AltName: Full=eIF-4F 25 kDa subunit {ECO:0000305};
DE AltName: Full=eIF-4F p26 subunit {ECO:0000305};
DE AltName: Full=mRNA cap-binding protein {ECO:0000303|PubMed:12857809};
GN Name=eIF4E {ECO:0000303|PubMed:12857809};
GN ORFNames=LSAT_4X27581 {ECO:0000312|EMBL:PLY83747.1};
OS Lactuca sativa (Garden lettuce).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae;
OC Lactucinae; Lactuca.
OX NCBI_TaxID=4236;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, FUNCTION (MICROBIAL INFECTION),
RP VARIANTS PRO-75; 113-GLN--ALA-115 DELINS HIS AND SER-191, SUBUNIT
RP (MICROBIAL INFECTION), AND POLYMORPHISM.
RC STRAIN=cv. 87-20M, cv. Floribibb, cv. Malika, cv. Mantilia, cv. Salinas,
RC cv. Salinas 88, cv. Vanguard, and cv. Vanguard 75;
RX PubMed=12857809; DOI=10.1104/pp.102.017855;
RA Nicaise V., German-Retana S., Sanjuan R., Dubrana M.-P., Mazier M.,
RA Maisonneuve B., Candresse T., Caranta C., LeGall O.;
RT "The eukaryotic translation initiation factor 4E controls lettuce
RT susceptibility to the Potyvirus Lettuce mosaic virus.";
RL Plant Physiol. 132:1272-1282(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Salinas;
RX PubMed=28401891; DOI=10.1038/ncomms14953;
RA Reyes-Chin-Wo S., Wang Z., Yang X., Kozik A., Arikit S., Song C., Xia L.,
RA Froenicke L., Lavelle D.O., Truco M.J., Xia R., Zhu S., Xu C., Xu H.,
RA Xu X., Cox K., Korf I., Meyers B.C., Michelmore R.W.;
RT "Genome assembly with in vitro proximity ligation data and whole-genome
RT triplication in lettuce.";
RL Nat. Commun. 8:14953-14953(2017).
RN [3]
RP GENE FAMILY, AND REVIEW.
RX PubMed=24309680; DOI=10.1016/j.meegid.2013.11.024;
RA Moury B., Charron C., Janzac B., Simon V., Gallois J.L., Palloix A.,
RA Caranta C.;
RT "Evolution of plant eukaryotic initiation factor 4E (eIF4E) and potyvirus
RT genome-linked protein (VPg): a game of mirrors impacting resistance
RT spectrum and durability.";
RL Infect. Genet. Evol. 27:472-480(2014).
CC -!- FUNCTION: Component of the protein complex eIF4F, which is involved in
CC the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal
CC secondary structure and recruitment of mRNA to the ribosome (By
CC similarity). Recognizes and binds the 7-methylguanosine-containing mRNA
CC cap during an early step in the initiation of protein synthesis and
CC facilitates ribosome binding by inducing the unwinding of the mRNAs
CC secondary structures (By similarity). Key component of recessive
CC resistance to potyviruses (PubMed:12857809).
CC {ECO:0000250|UniProtKB:P29557, ECO:0000269|PubMed:12857809}.
CC -!- FUNCTION: (Microbial infection) Susceptibility host factor required for
CC viral infection by recruiting viral RNAs to the host ribosomal complex
CC via an interaction with viral genome-linked protein (VPg).
CC {ECO:0000269|PubMed:12857809}.
CC -!- SUBUNIT: EIF4F is a multi-subunit complex, the composition of which
CC varies with external and internal environmental conditions. It is
CC composed of at least EIF4A, EIF4E and EIF4G. EIF4E is also known to
CC interact with other partners. In higher plants two isoforms of EIF4F
CC have been identified, named isoform EIF4F and isoform EIF(iso)4F.
CC Isoform EIF4F has subunits p220 and p26, whereas isoform EIF(iso)4F has
CC subunits p82 and p28. {ECO:0000250|UniProtKB:P29557}.
CC -!- SUBUNIT: (Microbial infection) Interacts with potyvirus viral genome-
CC linked protein (VPg); this interaction is possible in susceptible hosts
CC but impaired in resistant plants. {ECO:0000305|PubMed:12857809}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:C6ZJZ3}. Cytoplasm
CC {ECO:0000250|UniProtKB:C6ZJZ3}.
CC -!- PTM: According to the redox status, the Cys-133-Cys-171 disulfide
CC bridge may have a role in regulating protein function by affecting its
CC ability to bind capped mRNA. {ECO:0000250|UniProtKB:P29557}.
CC -!- POLYMORPHISM: Variant present in the strains cv. Autumn Gold, cv.
CC Desert Storm, cv. Salinas 88 and cv. Vanguard 75, alleles mo1(2) and
CC Ls-eIF4E(2), confers an increased resistance to lettuce mosaic virus
CC (LMV). {ECO:0000269|PubMed:12857809}.
CC -!- POLYMORPHISM: Variant present in the strains cv. Alize, cv. Classic,
CC cv. Floribibb, cv. Malika, cv. Mantilia, cv. Oriana and cv. Presidio,
CC alleles mo1(1) and Ls-eIF4E(1), confers an increased resistance to
CC lettuce mosaic virus (LMV). {ECO:0000269|PubMed:12857809}.
CC -!- MISCELLANEOUS: Displayed sequence is from cv. Salinas, cv. Fiona, cv.
CC Girelle, cv. Jessy, cv. Vanguard, cv. Mariska, cv. Trocadero and cv.
CC 87-20M, allele Ls-eIF4E(0), and is associated with susceptibility to
CC lettuce mosaic virus (LMV). {ECO:0000269|PubMed:12857809}.
CC -!- SIMILARITY: Belongs to the eukaryotic initiation factor 4E family.
CC {ECO:0000305}.
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DR EMBL; AF530162; AAP86602.1; -; mRNA.
DR EMBL; NBSK01004716; PLY83747.1; -; Genomic_DNA.
DR STRING; 4236.A0A2J6L8Y7; -.
DR EnsemblPlants; rna-gnl|WGS:NBSK|LSAT_4X27581_mrna; cds-PLY83747.1; gene-LSAT_4X27581.
DR Gramene; rna-gnl|WGS:NBSK|LSAT_4X27581_mrna; cds-PLY83747.1; gene-LSAT_4X27581.
DR OrthoDB; 1394271at2759; -.
DR Proteomes; UP000235145; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0016281; C:eukaryotic translation initiation factor 4F complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000340; F:RNA 7-methylguanosine cap binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR GO; GO:0003743; F:translation initiation factor activity; ISS:UniProtKB.
DR GO; GO:0051607; P:defense response to virus; ISS:UniProtKB.
DR GO; GO:0006413; P:translational initiation; ISS:UniProtKB.
DR Gene3D; 3.30.760.10; -; 1.
DR InterPro; IPR023398; TIF_eIF4e-like.
DR InterPro; IPR001040; TIF_eIF_4E.
DR InterPro; IPR019770; TIF_eIF_4E_CS.
DR PANTHER; PTHR11960; PTHR11960; 1.
DR Pfam; PF01652; IF4E; 1.
DR SUPFAM; SSF55418; SSF55418; 1.
DR PROSITE; PS00813; IF4E; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Disulfide bond; Host-virus interaction; Initiation factor;
KW Nucleus; Plant defense; Protein biosynthesis; Reference proteome;
KW RNA-binding; Translation regulation.
FT CHAIN 1..235
FT /note="Eukaryotic translation initiation factor 4E-1"
FT /id="PRO_0000454070"
FT REGION 16..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 60..63
FT /note="EIF4G-binding"
FT /evidence="ECO:0000250|UniProtKB:Q00LS8"
FT REGION 70..106
FT /note="EIF4G-binding"
FT /evidence="ECO:0000250|UniProtKB:Q00LS8"
FT REGION 154..163
FT /note="EIF4G-binding"
FT /evidence="ECO:0000250|UniProtKB:Q00LS8"
FT COMPBIAS 42..56
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 78..83
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT /ligand_part_id="ChEBI:CHEBI:74429"
FT /ligand_part_note="m7GTP residue in mRNA cap"
FT /evidence="ECO:0000250|UniProtKB:P29557"
FT BINDING 110
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT /ligand_part_id="ChEBI:CHEBI:74429"
FT /ligand_part_note="m7GTP residue in mRNA cap"
FT /evidence="ECO:0000250|UniProtKB:P29557"
FT BINDING 128..129
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT /ligand_part_id="ChEBI:CHEBI:74429"
FT /ligand_part_note="m7GTP residue in mRNA cap"
FT /evidence="ECO:0000250|UniProtKB:P29557"
FT BINDING 178..183
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT /ligand_part_id="ChEBI:CHEBI:74429"
FT /ligand_part_note="m7GTP residue in mRNA cap"
FT /evidence="ECO:0000250|UniProtKB:P29557"
FT BINDING 223..227
FT /ligand="mRNA"
FT /ligand_id="ChEBI:CHEBI:33699"
FT /ligand_part="N(7)-methylguanosine 5'-triphosphate group"
FT /ligand_part_id="ChEBI:CHEBI:74429"
FT /ligand_part_note="m7GTP residue in mRNA cap"
FT /evidence="ECO:0000250|UniProtKB:Q00LS8"
FT DISULFID 133..171
FT /evidence="ECO:0000250|UniProtKB:Q00LS8"
FT VARIANT 75
FT /note="A -> P (in strain: Autumn Gold, Desert Storm,
FT Salinas 88 and Vanguard 75, alleles mo1(2) and Ls-
FT eIF4E(2))"
FT /evidence="ECO:0000269|PubMed:12857809"
FT VARIANT 113..115
FT /note="QGA -> H (in strain: Alize, Classic, Floribibb,
FT Malika, Mantilia, Oriana and Presidio, alleles mo1(1) and
FT Ls-eIF4E(1))"
FT /evidence="ECO:0000269|PubMed:12857809"
FT VARIANT 191
FT /note="A -> S (in strain: Alize, Classic, Floribibb,
FT Malika, Mantilia, Oriana and Presidio, alleles mo1(1) and
FT Ls-eIF4E(1))"
FT /evidence="ECO:0000269|PubMed:12857809"
FT CONFLICT 1
FT /note="M -> G (in Ref. 1; AAP86602)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 235 AA; 26678 MW; D444A2B03451ABE0 CRC64;
MVEEIMKSEE QKLIDVNKHR GVRSDGEEDE QLEEGEIVGG DADTLSSSSS SRPGTAIAQH
PLEHSWTFWF DTPSAKSKQV AWGSSMRPIY TFSSVEEFWS LYNNIHRPSK LAQGADFYCF
KNKIEPKWED PVCANGGKWT MTFTKAKSDT CWLYTLLAMI GEQFDHGDDI CGAVVNVRAR
QEKIALWTKN AANESAQLSI GKQWKEFIDY NDTIGFIFHE DAKTLDRSAK NKYTV
